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Author: Paul N. MacDonald
Publisher: Springer Science & Business Media
ISBN: 1592592104
Category : Science
Languages : en
Pages : 337
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Book Description
The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Author: Paul N. MacDonald
Publisher: Springer Science & Business Media
ISBN: 1592592104
Category : Science
Languages : en
Pages : 337
Get Book Here
Book Description
The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Author: Jan Lunze
Publisher: Cambridge University Press
ISBN: 0521765056
Category : Computers
Languages : en
Pages : 583
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Book Description
Sets out core theory and reviews new methods and applications to show how hybrid systems can be modelled and understood.
Author: Emilio Corchado
Publisher: Springer
ISBN: 3642023193
Category : Computers
Languages : en
Pages : 736
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Book Description
The 4th International Conference on Hybrid Artificial Intelligence Systems (HAIS 2009), as the name suggests, attracted researchers who are involved in developing and applying symbolic and sub-symbolic techniques aimed at the construction of highly robust and reliable problem-solving techniques, and bringing the most relevant achievements in this field. Hybrid intelligent systems have become increasingly po- lar given their capabilities to handle a broad spectrum of real-world complex problems which come with inherent imprecision, uncertainty and vagueness, hi- dimensionality, and nonstationarity. These systems provide us with the opportunity to exploit existing domain knowledge as well as raw data to come up with promising solutions in an effective manner. Being truly multidisciplinary, the series of HAIS conferences offers an interesting research forum to present and discuss the latest th- retical advances and real-world applications in this exciting research field. This volume of Lecture Notes in Artificial Intelligence (LNAI) includes accepted papers presented at HAIS 2009 held at the University of Salamanca, Salamanca, Spain, June 2009. Since its inception, the main aim of the HAIS conferences has been to establish a broad and interdisciplinary forum for hybrid artificial intelligence systems and asso- ated learning paradigms, which are playing increasingly important roles in a large number of application areas.
Author: Paulo Tabuada
Publisher: Springer Science & Business Media
ISBN: 1441902244
Category : Science
Languages : en
Pages : 202
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Book Description
Hybrid systems describe the interaction of software, described by finite models such as finite-state machines, with the physical world, described by infinite models such as differential equations. This book addresses problems of verification and controller synthesis for hybrid systems. Although these problems are very difficult to solve for general hybrid systems, several authors have identified classes of hybrid systems that admit symbolic or finite models. The novelty of the book lies on the systematic presentation of these classes of hybrid systems along with the relationships between the hybrid systems and the corresponding symbolic models. To show how the existence of symbolic models can be used for verification and controller synthesis, the book also outlines several key results for the verification and controller design of finite systems. Several examples illustrate the different methods and techniques discussed in the book.
Author: Jan Awrejcewicz
Publisher: Springer Nature
ISBN: 3030773108
Category : Mathematics
Languages : en
Pages : 297
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Book Description
This volume is part of collection of contributions devoted to analytical and experimental techniques of dynamical systems, presented at the 15th International Conference “Dynamical Systems: Theory and Applications”, held in Łódź, Poland on December 2-5, 2019. The wide selection of material has been divided into three volumes, each focusing on a different field of applications of dynamical systems. The broadly outlined focus of both the conference and these books includes bifurcations and chaos in dynamical systems, asymptotic methods in nonlinear dynamics, dynamics in life sciences and bioengineering, original numerical methods of vibration analysis, control in dynamical systems, optimization problems in applied sciences, stability of dynamical systems, experimental and industrial studies, vibrations of lumped and continuous systems, non-smooth systems, engineering systems and differential equations, mathematical approaches to dynamical systems, and mechatronics.
Author: Robert Grossman
Publisher: Springer
ISBN:
Category : Computers
Languages : en
Pages : 488
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Book Description
"Hybrid systems are networks of interacting digital and analog devices. Control systems for inherently unstable aircraft and computer aided manufacturing are typical applications for hybrid systems, but due to the rapid development of processor and circuit technology modern cars and consumer electronics use software to control physical processes. The identifying characteristic of hybrid systems is that they incorporate both continuous components governed by differential equations and also digital components - digital computers, sensors, and actuators controlled by programs. This volume of invited refereed papers is inspired by a workshop on the Theory of Hybrid Systems, held at the Technical University, Lyngby, Denmark, in October 1992, and by a prior Hybrid Systems Workshop, held at Cornell University, USA, in June 1991, organized by R.L. Grossman and A. Nerode. Some papers are the final versions of papers presented at these workshops and some are invited papers from other researchers who were not able to attend these workshops."--PUBLISHER'S WEBSITE.
Author:
Publisher: Elsevier
ISBN: 0080453821
Category : Science
Languages : en
Pages : 7944
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Book Description
This work presents a definitive interpretation of the current status of and future trends in natural products—a dynamic field at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids, and enzymes. With more than 1,800 color figures, Comprehensive Natural Products II features 100% new material and complements rather than replaces the original work (©1999). Reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine Stimulates new ideas among the established natural products research community—which includes chemists, biochemists, biologists, botanists, and pharmacologists Informs and inspires students and newcomers to the field with accessible content in a range of delivery formats Includes 100% new content, with more than 6,000 figures (1/3 of these in color) and 40,000 references to the primary literature, for a thorough examination of the field Highlights new research and innovations concerning living organisms and their distinctive role in our understanding and improvement of human health, genomics, ecology/environment, and more Adds to the rich body of work that is the first edition, which will be available for the first time in a convenient online format giving researchers complete access to authoritative Natural Products content
Author: Danuta Rutkowska
Publisher: Physica
ISBN: 379081802X
Category : Computers
Languages : en
Pages : 292
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Book Description
The advent of the computer age has set in motion a profound shift in our perception of science -its structure, its aims and its evolution. Traditionally, the principal domains of science were, and are, considered to be mathe matics, physics, chemistry, biology, astronomy and related disciplines. But today, and to an increasing extent, scientific progress is being driven by a quest for machine intelligence - for systems which possess a high MIQ (Machine IQ) and can perform a wide variety of physical and mental tasks with minimal human intervention. The role model for intelligent systems is the human mind. The influ ence of the human mind as a role model is clearly visible in the methodolo gies which have emerged, mainly during the past two decades, for the con ception, design and utilization of intelligent systems. At the center of these methodologies are fuzzy logic (FL); neurocomputing (NC); evolutionary computing (EC); probabilistic computing (PC); chaotic computing (CC); and machine learning (ML). Collectively, these methodologies constitute what is called soft computing (SC). In this perspective, soft computing is basically a coalition of methodologies which collectively provide a body of concepts and techniques for automation of reasoning and decision-making in an environment of imprecision, uncertainty and partial truth.
Author: Christos G. Cassandras
Publisher: CRC Press
ISBN: 1420008544
Category : Technology & Engineering
Languages : en
Pages : 301
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Book Description
Because they incorporate both time- and event-driven dynamics, stochastic hybrid systems (SHS) have become ubiquitous in a variety of fields, from mathematical finance to biological processes to communication networks to engineering. Comprehensively integrating numerous cutting-edge studies, Stochastic Hybrid Systems presents a captivating treatment of some of the most ambitious types of dynamic systems. Cohesively edited by leading experts in the field, the book introduces the theoretical basics, computational methods, and applications of SHS. It first discusses the underlying principles behind SHS and the main design limitations of SHS. Building on these fundamentals, the authoritative contributors present methods for computer calculations that apply SHS analysis and synthesis techniques in practice. The book concludes with examples of systems encountered in a wide range of application areas, including molecular biology, communication networks, and air traffic management. It also explains how to resolve practical problems associated with these systems. Stochastic Hybrid Systems achieves an ideal balance between a theoretical treatment of SHS and practical considerations. The book skillfully explores the interaction of physical processes with computerized equipment in an uncertain environment, enabling a better understanding of sophisticated as well as everyday devices and processes.
Author: André Platzer
Publisher: Springer Science & Business Media
ISBN: 3642145094
Category : Mathematics
Languages : en
Pages : 446
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Book Description
Hybrid systems are models for complex physical systems and have become a widely used concept for understanding their behavior. Many applications are safety-critical, including car, railway, and air traffic control, robotics, physical–chemical process control, and biomedical devices. Hybrid systems analysis studies how we can build computerized controllers for physical systems which are guaranteed to meet their design goals. The author gives a unique, logic-based perspective on hybrid systems analysis. It is the first book that leverages the power of logic for hybrid systems. The author develops a coherent logical approach for systematic hybrid systems analysis, covering its theory, practice, and applications. It is further shown how the developed verification techniques can be used to study air traffic and railway control systems. This book is intended for researchers, postgraduates, and professionals who are interested in hybrid systems analysis, cyberphysical or embedded systems design, logic and theorem proving, or transportation and automation.
