High Resolution Separation of Small DNA Restriction Fragments by Capillary Electrophoresis Using Hydroxyethyl Cellulose as a Buffer Additive PDF Download
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Author: Deanne Lyn Hertzog
Publisher:
ISBN:
Category :
Languages : en
Pages : 228
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Author: Deanne Lyn Hertzog
Publisher:
ISBN:
Category :
Languages : en
Pages : 228
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Author: Christoph Heller
Publisher: Springer Science & Business Media
ISBN: 3322910156
Category : Technology & Engineering
Languages : en
Pages : 318
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Book Description
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
Author: Annelise Emily Barron
Publisher:
ISBN:
Category :
Languages : en
Pages : 704
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 20
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Because the mechanism of DNA separation in capillary electrophoresis is not well understood, selection of polymers is a {open_quotes}trial-and-error{close_quotes} procedure. We investigated dilute-solution DNA separations by capillary electrophoresis using solutions of four polymers that differ in size, shape and stiffness. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp and star-(polyethylene oxide), like linear poly (ethylene oxide), provides separation of fragments up to 1353 bp.
Author: Wade Michael Sunada
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 390
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Author: Keith R. Mitchelson
Publisher: Humana
ISBN: 9781617371776
Category : Science
Languages : en
Pages : 0
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The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Author: Cheuk Wai Kan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Capillary electrophoresis (CE) in the presence of entangled, uncrosslinked polymer solutions is the dominant technology for several important genetic analyses that rely upon the size-based separation of DNA fragments. There is a growing interest in transforming the current CE technology into a microfabricated format for DNA sequencing and genotyping, for higher throughput and lower cost. This research aims at designing high-performance thermo-responsive polymers and copolymers of acrylamide derivatives for DNA sequencing and genotyping and at applying our prior knowledge in CE-based DNA analysis to a microchip-based method. Recent advances in DNA sequencing and genotyping, in miniaturized electrophoresis systems are critically reviewed. Polymers and copolymers of N-ethoxyethylacrylamide and N-methoxyethylacrylamide were synthesized, the polymer physical properties were carefully investigated, and the DNA sequencing performances of these polymer matrices were evaluated. Rheometry results suggest that these polymers undergo a sol-gel transition from viscous solutions to elastic, solid-like materials upon heating. DNA sequencing studies indicate that the physical stability brought about by thermo-gelation enhances single-stranded DNA separation resolution and hence extends the DNA sequencing read length relative to a non-thermogelling control matrix. A different approach to enhancing double-stranded DNA separation resolution was taken in an investigation using blends of two well-studied cellulose derivatives, hydroxyethylcellulose and hydroxypropylcellulose, to create a DNA sieving matrix with a thermally tunable mesh size for dsDNA separation. The thermo-responsive constituent of this polymer blend (hydroxypropylcellulose) allows the mesh size of the sieving matrix to be thermally controlled, to facilitate the optimal separation of DNA fragments over different size ranges, which is not easily attainable with conventional polymer sieving matrices. The approach was extended to blends of thermo-responsive N,N-diethylacrylamide and N,N-dimethylacrylamide copolymers. (Abstract shortened by UMI.)
Author: James P. Landers
Publisher: CRC Press
ISBN:
Category : Medical
Languages : en
Pages : 690
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Book Description
The Handbook of Capillary Electrophoresis is a valuable collection of in-depth discussions of specific areas of capillary electrophoresis (CE) that are key to the practical use of CE for sample analysis. The handbook is divided into five parts. Part I focuses on various modes of CE that have been developed and applied to analytical/separation problems. Part II covers detection in CE, with one section reviewing all detector systems that have been successfully interfaced with CE and another that focuses on CE-mass spectrometry. Part III explores how CE can be applied to the analysis of a specific class of molecules/analytes or to solving separation problems. CE analysis of organic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments; the components of single cells; and the utility of capillary coatings in CE are all covered in this section. A key element of this handbook is its discussion of the potential role of CE for solving clinical problems and the potential for CE and CE-MS to advance analysis of drug metabolism. The handbook also presents practical and theoretical discussions on various aspects of CE, including sample matrix and its effects on separation, the need for controlling capillary temperature, and the present and future implications of EOF control. In-depth discussions of this nature comprise Parts IV and V. The Handbook of Capillary Electrophoresis will be an outstanding reference for analytical chemists, biochemists, clinical chemists, cell biologists, and pharmaceutical researchers.
Author: Dietmar Tietz
Publisher: Springer Science & Business Media
ISBN: 3642589243
Category : Science
Languages : en
Pages : 341
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Book Description
Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described here in detail. Leading experts present the required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers' addresses, helpful Internet resources, as well as specific applications, e.g. in legal medicine, microbiology and agriculture.
Author: Yongseong Kim
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
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Book Description
