High Performance Liquid Chromatography in Phytochemical Analysis

High Performance Liquid Chromatography in Phytochemical Analysis PDF Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 9781420092615
Category : Health & Fitness
Languages : en
Pages : 996

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Book Description
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

High Performance Liquid Chromatography in Phytochemical Analysis

High Performance Liquid Chromatography in Phytochemical Analysis PDF Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 9781420092615
Category : Health & Fitness
Languages : en
Pages : 996

Get Book

Book Description
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp

High Performance Liquid Chromatography in Plant Sciences

High Performance Liquid Chromatography in Plant Sciences PDF Author: Hans-Ferdinand Linskens
Publisher: Springer Science & Business Media
ISBN: 3642829511
Category : Science
Languages : en
Pages : 267

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Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.

High-Resolution Mass Spectroscopy for Phytochemical Analysis

High-Resolution Mass Spectroscopy for Phytochemical Analysis PDF Author: Sreeraj Gopi
Publisher: CRC Press
ISBN: 1000353613
Category : Science
Languages : en
Pages : 240

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Book Description
This new volume provides a bird’s-eye view of the properties, utilization, and importance of high resolution mass spectrometry (HRMS) for phytochemical analysis. The book discusses the new and state-of-the-art technologies related to HRMS in phytochemical analysis for the food industry in a comprehensive manner. Phytochemical characterization of plants is important in the food and nutraceutical industries and is also necessary in the procedures followed for drug development, toxicology determination, forensic studies, origin verification, quality assurance, etc. Easy determination of active compounds and isolation as well as purification of the same from natural matrices are required, and the possibilities and advantages of HRMS pave the way for improved analysis patterns in phytochemistry. This book is unique in that its sole consideration is on the importance of HRMS in the field of phytochemical analysis. Along with an overview of basic instrumental information, the volume provides a detailed account of data processing and dereplication strategies. Technologies such as bioanalytical techniques and bioassays are considered also to provide support for the functions of the instruments used. In addition, a case study is presented to depict the complete phytochemical characterization of a matrix by HRMS. The book covers processing and computational techniques, dereplication, hyphenation, high-resolution bioassays, bioanalytical screening/purification techniques, applications of gas chromatography–high-resolution mass spectrometry, and more. Key features: Covers the fundamental instrumentation and techniques Discusses HRMS-based phytochemical research details Focuses strictly on the phytochemical considerations High-Resolution Mass Spectroscopy for Phytochemical Analysis: State-of-the-Art Applications and Techniques will be a valuable reference guide and resource for researchers, faculty and students in related fields, as well as those in the phytochemical industries.

Analysis of Antioxidant-Rich Phytochemicals

Analysis of Antioxidant-Rich Phytochemicals PDF Author: Zhimin Xu
Publisher: John Wiley & Sons
ISBN: 0813823919
Category : Technology & Engineering
Languages : en
Pages : 405

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Book Description
To quantify antioxidants in natural sources, the application of chromatography techniques with different detectors followed by skillful sample preparation is necessary. Analysis of Antioxidant-Rich Phytochemicals is the first book that specifically covers and summarizes the details of sample preparation procedures and methods developed to identify and quantify various types of natural antioxidants in foods. Focusing on the principle of quantification methods for natural antioxidants, the book reviews and summarizes current methods used in the determination of antioxidant-rich phytochemicals in different sources. Chapter by chapter, the distinguished team of authors describes the various methods used for analysis of the different antioxidant-rich phytochemicals – phenolic acids; carotenoids; anthocyanins; ellagitannins, flavonols and flavones; catechins and procyanidins; flavanones; stilbenes; phytosterols; and tocopherols and tocotrienols. Going beyond extensive reviews of the scientific literature, the expert contributors call on their accumulated experience in sample extraction and analysis to outline procedures, identify potential problems in dealing with different samples, and offer trouble-shooting tips for the analysis. Analysis of Antioxidant-Rich Phytochemicals covers the important food applications and health-promoting functions of the major antioxidant phytochemicals, presents general analysis principles and procedures, and systematically reviews and summarizes the various analytical methods necessary for each type of natural antioxidant in different food sources.

Thin Layer Chromatography in Phytochemistry

Thin Layer Chromatography in Phytochemistry PDF Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 9781420046786
Category : Science
Languages : en
Pages : 896

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Book Description
Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Phytochemical Analysis

Phytochemical Analysis PDF Author: Dr. Vinesh Kumar
Publisher: Booktango
ISBN: 1468972111
Category : Education
Languages : en
Pages : 27

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Book Description
The aim of this book is to provide the brief introduction of the techniques used for phytochemical studies. This book includes the methods used for plant material collection, their storage, extraction, isolation, and identification of organic constituents present in plant materials under study.

Phytochemical Techniques (2nd Revised And Enlarged Edition)

Phytochemical Techniques (2nd Revised And Enlarged Edition) PDF Author: Prof. Dr. N. Raaman
Publisher: New India Publishing Agency
ISBN: 939017595X
Category : Technology & Engineering
Languages : en
Pages : 12

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Book Description
Phytochemicals are the individual chemicals from which the plants are made and plants are the key sources of raw materials for both pharmaceutical and aromatic industries. The improved methods for higher yield of active compounds will be the major incentive in these industries. To help those who involved in the isolation of compounds from plants, some of the essential phytochemical techniques are included in this book. It contains 10 chapters. A brief introduction is given in Chapter 1. Chapter 2 deals with the production processes for herbals and botanicals. Selection of plant and plant parts for phytochemical analysis are included in Chapter 3. Different methods of extraction are given in Chapter 4. Qualitative phytochemical screening is presented in Chapter 5. Various methods for separation of phytochemicals, which include paper and thin layer chromatography and column chromatography are given in Chapter 6. Qualitative and quantitative estimation of phytochemicals using gas chromatography, high performance liquid chromatography and high performance thin layer chromatography are described in Chapter 7. The various methods of identification including the physical characteristics and spectroscopy are included in Chapter 8. The ultraviolet spectroscopy, infrared spectroscopy, near infrared spectroscopy, mass spectroscopy, nuclear magnetic resonance spectroscopy and crystallography are included in this chapter. The categories of phytochemicals are given in Chapter 9. A case study of isolation and identification of compounds in the laboratory of the author of this book is included in Chapter 10. Isolation of alkaloids is given in Chapter 11. Extraction and isolation of phenolic compounds is described in Chapter 12. Isolation of anthocyanin compounds is included in Chapter 13. Extraction and analysis of essential oils are described in Chapter 14. The theoretical principles involved in the instruments, handling of samples and interpretation of spectra are given in detail. More than 160 figures (27 in colour) are included to illustrate the various techniques and the structures of compounds. Apart from the references, indexes of common and scientific names of plants and chemical names and subject index are included.

Modern Phytochemical Methods

Modern Phytochemical Methods PDF Author: Nikolaus H. Fischer
Publisher: Springer Science & Business Media
ISBN: 1468490605
Category : Science
Languages : en
Pages : 412

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Book Description
This volume contains reviews which are based on a symposium, given th at the 30 meeting of The Phytochemical Society of North America, held at Laval University in Quebec City, Canada on August 11-15, 1990. During the past two decades, there have been major new developments in methods which can be applied toward the isolation, separation and structure determination of complex natural products. Therefore, the topic of this symposium, "Modem Phytochemical Methods", is a very timely one. The organizers of the symposium recognized that it would not be possible to cover in detail all new advances in phytochemical methodology. It was therefore decided to emphasize general reviews on recent developments of major separation techniques such as high performance liquid chromatography as well as supercritical fluid chromato graphy. In addition, advances in commonly used structure determination methods, mainly NMR and MS, are reviewed. Other topics include methodo logies of micro-sampling for isolation and analysis of trichome constituents as well as recent breakthroughs on biosynthetic studies of monoterpenes using "enriched" basal cells of trichomes. The volume concludes with a review of quantitative structure-activity relationship (QSAR) studies of biologically active natural products. In Chapter I, K. Hostettmann and his colleagues give a general review of recent developments in the separation of natural products with major emphasis on preparative separations of biologically active plant constituents. The authors present a comparison of droplet countercurrent chromatography (OCCC) with the highly rapid and more versatile centrifugal partition chromatography (CPC).

Phytochemistry of Medicinal Plants

Phytochemistry of Medicinal Plants PDF Author: John T. Arnason
Publisher: Springer Science & Business Media
ISBN: 1489917780
Category : Science
Languages : en
Pages : 369

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Book Description
Phytochemicals from medicinal plants are receiving ever greater attention in the scientific literature, in medicine, and in the world economy in general. For example, the global value of plant-derived pharmaceuticals will reach $500 billion in the year 2000 in the OECD countries. In the developing countries, over-the-counter remedies and "ethical phytomedicines," which are standardized toxicologically and clinically defined crude drugs, are seen as a promising low cost alternatives in primary health care. The field also has benefited greatly in recent years from the interaction of the study of traditional ethnobotanical knowledge and the application of modem phytochemical analysis and biological activity studies to medicinal plants. The papers on this topic assembled in the present volume were presented at the annual meeting of the Phytochemical Society of North America, held in Mexico City, August 15-19, 1994. This meeting location was chosen at the time of entry of Mexico into the North American Free Trade Agreement as another way to celebrate the closer ties between Mexico, the United States, and Canada. The meeting site was the historic Calinda Geneve Hotel in Mexico City, a most appropriate site to host a group of phytochemists, since it was the address of Russel Marker. Marker lived at the hotel, and his famous papers on steroidal saponins from Dioscorea composita, which launched the birth control pill, bear the address of the hotel.

High Performance Liquid Chromatography in Plant Sciences

High Performance Liquid Chromatography in Plant Sciences PDF Author: Hans-Ferdinand Linskens
Publisher: Springer
ISBN: 9783540172437
Category : Science
Languages : en
Pages : 248

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Book Description
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.