Glycovirology Protocols PDF Download
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Author: Richard J. Sugrue
Publisher: Springer Science & Business Media
ISBN: 1597453935
Category : Medical
Languages : en
Pages : 426
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Book Description
This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins. The information presented here provides insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.
Author: Richard J. Sugrue
Publisher: Springer Science & Business Media
ISBN: 1597453935
Category : Medical
Languages : en
Pages : 426
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Book Description
This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins. The information presented here provides insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.
Author: Inka Brockhausen
Publisher: Springer Science & Business Media
ISBN: 1597451673
Category : Science
Languages : en
Pages : 388
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Book Description
Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell.
Author: Yasuo Suzuki
Publisher: Springer Nature
ISBN: 1071626353
Category : Medical
Languages : en
Pages : 363
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Book Description
This volume details applications in molecular biological techniques and focuses on applications to determine the involvement of glycans in virus interactions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycovirology: Methods and Protocols aims to be an essential resource for researchers who wish to learn more about glycovirology.
Author: Thomas Lütteke
Publisher: Humana
ISBN: 9781493941957
Category : Science
Languages : en
Pages : 0
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Book Description
This book provides current glycoinformatics methods and protocols used to support the determination of carbohydrate structures in biological samples as well as carbohydrate structure databases, the interaction of carbohydrates with proteins, and theoretical and experimental methods to study their three-dimensional structure and dynamics. Glycoinformatics explores this recently emerged field, which has come into being in order to address the needs of encoding, storing, and analyzing carbohydrate ‘sequences’ and their taxonomy using computers. Written in the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and key implementation advice to ensure successful results. Authoritative and timely, Glycoinformatics demonstrates the progress that has been achieved in glycoinformatics, which indicates that it is no longer a niche subject covered by only a few scientists but is truly coming of age.
Author: Adeel Malik
Publisher: CRC Press
ISBN: 1000344320
Category : Science
Languages : en
Pages : 321
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Book Description
This volume provides a comprehensive understanding of the enigmatic identity of the glycome, a complex but important area of research that has been largely ignored due to its complexity. The authors thoroughly deal with almost all aspects of the glycome, i.e., elucidation of the glycan identity enigma and its role in regulation of the cellular process, and in disease etiology. The book bridges the knowledge gap in understanding the glycome, from being a cell signature to its applications in disease etiology. In addition, it details many of the major insights regarding the possible role of the glycome in various diseases as a therapeutic marker. The book systematically covers the major aspects of the glycome, including the significance of substituting the diverse monosaccharide units to glycoproteins, the role of glycans in disease pathologies, and the challenges and advances in glycobiology. The authors stress the significance and huge encoding power of carbohydrates as well as provide helpful insights in framing the bigger picture. The Glycome: Understanding the Diversity and Complexity of Glycobiology details state-of-the-art developments and emerging challenges of glycome biology, which are going to be key areas of future research, not only in the glycobiology field but also in pharmaceutics.
Author: Ajit Varki
Publisher: CSHL Press
ISBN: 9780879696818
Category : Medical
Languages : en
Pages : 694
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Book Description
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
Author: Maureen E. Taylor
Publisher: Oxford University Press, USA
ISBN: 0199569118
Category : Medical
Languages : en
Pages : 305
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Book Description
Introduction to Glycobiology reveals the true impact of the sugars on biological systems, explaining their function at the molecular, cellular, and organismal level and their clinical relevance.
Author: Renato V. Iozzo
Publisher: Springer Science & Business Media
ISBN: 1592592090
Category : Science
Languages : en
Pages : 547
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Book Description
Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.
Author: Els J. M. Van Damme
Publisher: Frontiers Media SA
ISBN: 2889194213
Category : Botany
Languages : en
Pages : 137
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Book Description
Plants synthesize a wide variety of unique glycan structures which play essential roles during the life cycle of the plant. Being omnipresent throughout the plant kingdom, ranging from simple green algae to modern flowering plants, glycans contribute to many diverse processes. Glycans can function as structural components in the plant cell wall, assist in the folding of nascent proteins, act as signaling molecules in plant defense responses or (ER) stress pathways, or serve within the energy metabolism of a plant. In most cases, glycans are attached to other macromolecules to form so-called glycoconjugates (e.g. glycoproteins, proteoglycans and glycolipids), but they can also be present as free entities residing in the plant cell. Next to the broad, complex set of glycans, plants also evolved an elaborate collection of lectins or proteins with a lectin-like domain, which can recognize and bind to endogenous (plants-own) or exogenous (foreign) glycans. Though still poorly understood in plants, the dynamic interactions between lectins and carbohydrate structures are suggested to be involved in gene transcription, protein folding, protein transport, cell adhesion, signaling as well as defense responses. As such, a complex and largely undetermined glycan-interactome is established inside plant cells, between cells and their surrounding matrix, inside the extracellular matrix, and even between organisms. Studying the biological roles of plant glycans will enable to better understand plant development and physiology in order to fully exploit plants for food, feed and production of pharmaceutical proteins. In this Research Topic, we want to provide a platform for articles describing the latest research, perspectives and methodologies related to the fascinating world of plant glycobiology, with a focus on following subjects: 1. Identification and characterization of plant glycans, their biosynthetic and degradation enzymes 2. Characterization of plant lectins and glycoproteins 3. Plant glycans in the plant’s energy metabolism 4. Role of plant glycans in plant defense signaling 5. Use of plant lectins in pest control 6. Plant lectins as new tools in human medicine 7. Glyco-engineering in plants
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309260868
Category : Science
Languages : en
Pages : 171
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Book Description
A new focus on glycoscience, a field that explores the structures and functions of sugars, promises great advances in areas as diverse as medicine, energy generation, and materials science, this report finds. Glycans-also known as carbohydrates, saccharides, or simply as sugars-play central roles in many biological processes and have properties useful in an array of applications. However, glycans have received little attention from the research community due to a lack of tools to probe their often complex structures and properties. Transforming Glycoscience: A Roadmap for the Future presents a roadmap for transforming glycoscience from a field dominated by specialists to a widely studied and integrated discipline, which could lead to a more complete understanding of glycans and help solve key challenges in diverse fields.
