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Author: Ronald P. van Rij
Publisher: Humana Press
ISBN: 9781617790362
Category : Medical
Languages : en
Pages : 0
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Book Description
Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Author: Ronald P. van Rij
Publisher: Humana Press
ISBN: 9781617790362
Category : Medical
Languages : en
Pages : 0
Get Book Here
Book Description
Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.
Author: Ute Schepers
Publisher: John Wiley & Sons
ISBN: 3527604375
Category : Science
Languages : en
Pages : 336
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Book Description
This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Author: Robert F. Schleif
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 724
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Book Description
In the first edition of Genetics and Molecular Biology, renowned researcher and award-winning teacher Robert Schleif produced a unique and stimulating text that was a notable departure from the standard compendia of facts and observations. Schleif's strategy was to present the underlying fundamental concepts of molecular biology with clear explanations and critical analysis of well-chosen experiments. The result was a concise and practical approach that offered students a real understanding of the subject. This second edition retains that valuable approach--with material thoroughly updated to include an integrated treatment of prokaryotic and eukaryotic molecular biology. Genetics and Molecular Biology is copiously illustrated with two-color line art. Each chapter includes an extensive list of important references to the primary literature, as well as many innovative and thought-provoking problems on material covered in the text or on related topics. These help focus the student's attention of a variety of critical issues. Solutions are provided for half of the problems. Praise for the first edition: "Schleif's Genetics and Molecular Biology... is a remarkable achievement. It is an advanced text, derived from material taught largely to postgraduates, and will probably be thought best suited to budding professionals in molecular genetics. In some ways this would be a pity, because there is also gold here for the rest of us... The lessons here in dealing with the information explosion in biology are that an ounce of rationale is worth a pound of facts and that, for educational value, there is nothing to beat an author writing about stuff he knows from theinside."--Nature. "Schleif presents a quantitative, chemically rigorous approach to analyzing problems in molecular biology. The text is unique and clearly superior to any currently available."--R.L. Bernstein, San Francisco State University. "The greatest strength is the author's ability to challenge the student to become involved and get below the surface."--Clifford Brunk, UCLA
Author: Noureddine Benkeblia
Publisher: John Wiley & Sons
ISBN: 1119079942
Category : Technology & Engineering
Languages : en
Pages : 549
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Book Description
An in-depth treatment of cutting-edge work being done internationally to develop new techniques in crop nutritional quality improvement Phytonutritional Improvement of Crops explores recent advances in biotechnological methods for the nutritional enrichment of food crops. Featuring contributions from an international group of experts in the field, it provides cutting-edge information on techniques of immense importance to academic, professional and commercial operations. World population is now estimated to be 7.5 billion people, with an annual growth rate of nearly 1.5%. Clearly, the need to enhance not only the quantity of food produced but its quality has never been greater, especially among less developed nations. Genetic manipulation offers the best prospect for achieving that goal. As many fruit crops provide proven health benefits, research efforts need to be focused on improving the nutritional qualities of fruits and vegetables through increased synthesis of lycopene and beta carotene, anthocyanins and some phenolics known to be strong antioxidants. Despite tremendous growth in the area occurring over the past several decades, the work has only just begun. This book represents an effort to address the urgent need to promote those efforts and to mobilise the tools of biotechnical and genetic engineering of the major food crops. Topics covered include: New applications of RNA-interference and virus induced gene silencing (VIGS) for nutritional genomics in crop plants Biotechnological techniques for enhancing carotenoid in crops and their implications for both human health and sustainable development Progress being made in the enrichment and metabolic profiling of diverse carotenoids in a range of fruit crops, including tomatoes, sweet potatoes and tropical fruits Biotechnologies for boosting the phytonutritional values of key crops, including grapes and sweet potatoes Recent progress in the development of transgenic rice engineered to massively accumulate flavonoids in-seed Phytonutritional Improvement of Crops is an important text/reference that belongs in all universities and research establishments where agriculture, horticulture, biological sciences, and food science and technology are studied, taught and applied.
Author: R. K. Gaur
Publisher: CRC Press
ISBN: 1439873259
Category : Science
Languages : en
Pages : 388
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Book Description
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA
Author: Lesley J. Collins
Publisher: Springer Science & Business Media
ISBN: 1461403324
Category : Medical
Languages : en
Pages : 297
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Book Description
RNAs form complexes with proteins and other RNAs. The RNA‐infrastructure represents the spatiotemporal interaction of these proteins and RNAs in a cell‐wide network. RNA Infrastructure and Networks brings together these ideas to illustrate the scope of RNA‐based biology, and how connecting RNA mechanisms is a powerful tool to investigate regulatory pathways. This book is but a taste of the wide range of RNA‐based mechanisms that connect in the RNA infrastructure.
Author: M.A. Matzke
Publisher: Springer Science & Business Media
ISBN: 9401141835
Category : Science
Languages : en
Pages : 297
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Book Description
This book is an up-to-date and comprehensive collection of reviews on various aspects of epigenetic gene silencing in plants. Research on this topic has undergone explosive growth during the past decade and has revealed novel features of gene regulation and plant defense responses that also apply to animals and fungi. Gene silencing is relevant for agricultural biotechnology because stable expression of transgenes is required for the successful commercialization of genetically engineered crops. The reviews have been written by distinguished authors who have made significant contributions to plant gene silencing research. This volume supersedes other books on gene silencing by focussing on plant systems, where many pioneering experiments have been performed, and by including the latest developments from top laboratories. The book is geared toward advanced students of genetics and plant sciences as well as applied and basic research scientists who work with transgenic organisms and epigenetic regulation of gene expression.
Author: Henry F. Epstein
Publisher: Academic Press
ISBN: 0125641494
Category : Caenorhabditis elegans
Languages : en
Pages : 687
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Book Description
The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.
Author: Alfonso J. Rodriguez-Morales
Publisher: BoD – Books on Demand
ISBN: 1789848245
Category : Medical
Languages : en
Pages : 179
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Book Description
Tropical emerging diseases pose a significant risk for the circulation of old and new pathogens in areas previously unknown, also implying the possibility of new morbidities and mortalities and new consequences for naïve populations. Globalization, migration and travel are key factors for tropical diseases, and represent the need for integration of tropical medicine, travel medicine and epidemiology in the understanding of such complex situations. Neglected tropical diseases such as leprosy or Chagas disease, arboviral diseases, HIV, Ebola, and arenaviral infections are just a few examples. This book tries to update significant epidemiological and clinical research in many aspects with a multinational perspective.
Author: Jonatha M. Gott
Publisher: Springer Science & Business Media
ISBN: 1592597750
Category : Science
Languages : en
Pages : 437
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Book Description
This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.
