Genetic and Biochemical Characterization of Drosophila U2 SnRNP Auxiliary Factor PDF Download
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Author: David Zelcer Rudner
Publisher:
ISBN:
Category :
Languages : en
Pages : 480
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Author: David Zelcer Rudner
Publisher:
ISBN:
Category :
Languages : en
Pages : 480
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Book Description
Author: Kavita V. S. Vadali
Publisher:
ISBN: 9781109884050
Category : Drosophila
Languages : en
Pages : 250
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Book Description
Drosophila protein tyrosine phosphatase Pez belongs to the FERM superfamily of proteins, many of which interact with the actin cytoskeleton. Due to the presence of the FERM and phosphatase domains, PEZ-class proteins are suspected to play a role in tumor metastasis. The aim of this study is to identify the cellular processes in which Pez participates, using molecular genetic and biochemical approaches. The subcellular distribution of Pez was examined by immunohistochemistry; endogenous Pez cannot be detected, so a transgene that can overexpress Pez in a tissue specific manner was employed to produce detectable amounts of the protein. Pez forms aggregates at the apical surface of epithelial cells. Pez does not colocalize with adherens junction proteins, as seen for human Pez, nor with several cytoskeletal proteins. Ectopic Pez in developing wing epithelia causes a reduction in cell proliferation. Pez does not genetically antagonize tyrosine kinase pathways, as predicted for a phosphatase. Loss-of-function studies show that Pez is required for proper limb development, but not for viability or developmental patterning. A genetic screen was used to identify several regions of the genome that, when present in just one copy, modify the phenotypes due to ectopic Pez in the wings and eyes. Finally, the bimolecular fluorescence complementation assay was used to show that the Drosophila protein Aru can bind Pez in vivo. Together, the findings have ruled out several major hypotheses for Pez function: it does not significantly regulate cell shape or motility, it does not generally counteract tyrosine kinases and is not essential for their patterning functions, and it does not have an abundant binding site in the adherens junction.
Author: Gustavo Maroni
Publisher: Oxford University Press
ISBN: 0198023529
Category : Science
Languages : en
Pages : 428
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Book Description
Drosophila, the common fruit fly, is the most extensively studied of all organisms from the standpoint of genetics and cytology. This atlas summarizes what is known about the approximately 100 Drosophila genes for which the complete nucleotide sequence is known. Each entry includes a description of the gene's molecular organization and expression, the complete nucleotide and amino acid sequences, maps of interesting structures, highlights of functional features and promoter regulatory regions, and selected references to the primary literature. A separate section of the atlas considers different aspects of gene organization as they occur in the Drosophila genome. Topics covered include size correlations among various genetic elements, splicing signals, translation initiation signals, and codon bias. The work represents a new milestone in summarizing current information and making it easily accessible to geneticists and biologists.
Author: Jeffrey C. Hall
Publisher: Elsevier
ISBN: 9780120176311
Category : Science
Languages : en
Pages : 308
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Book Description
Under the leadership of Dr. Jeffrey C. Hall and Dr. Jay C. Dunlap, Advances in Genetics covers all genetic systems-from prokaryotic to human-with an eye toward identifying emerging problems as they coalesce. Volumes present both synoptic and topical reviews in a comprehensible, informative, and insightful manner. Articles range from detailed and discursive to brief and sharply focused, as benefits the problem under study. Founded in 1946 by Dr. Miloslav Demerc, this serial publication continues to be critical reading for researchers who need current overviews of areas outside their specialty and for students who need comprehensive introduction to topics genetic research. Under new editorial direction beginning with this volume Publishes comprehensive and timely chapters of genetic Delves into genetic phenomena at a molecular level Ties together related issues in cell and developmental biology and neurobiology
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 816
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Author: Huijun Zhou Ring
Publisher:
ISBN:
Category :
Languages : en
Pages : 294
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Book Description
Author: Joe B. Harford
Publisher: John Wiley & Sons
ISBN: 9780471142065
Category : Science
Languages : en
Pages : 372
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Book Description
mRNA METABOLISM & POST-TRANSCRIPTIONAL GENE REGULATION Edited by Joe B. Harford and David R. Morris Gene expression is a process that begins with the transcription ofDNA to an RNA messenger (mRNA), which is then translated into aprotein. Historically, attention has been focused on the regulationof RNA synthesis (transcription); however, there is a growingrecognition of and appreciation for the importance of the manyregulatory mechanisms that take place after RNA synthesis has beencompleted. mRNA Metabolism and Post-Transcriptional Gene Regulation is thefirst comprehensive overview of the various modes of generegulation that exist post-transcriptionally. Collecting studies bysome of the top researchers in the field, this volume provides bothan up-to-date review of the complex "life" of an mRNA molecule andan introduction to current work on the diversity of mechanisms ofpost-transcriptional reactions. Topics covered include: * RNA structure * Mammalian RNA editing * RNA export from the nucleus * The fundamentals of translation initiation * Control of mRNA decay in plants * mRNA metabolism and cancer * Control of mRNA stability during herpes simplex virus infection * Regulation of mRNA expression in HIV-1 and other complexretroviruses * Nucleases * RNA localization A timely contribution to the understanding of genetic regulatorymechanisms, mRNA Metabolism and Post-Transcriptional GeneRegulation provides a basis from which potential therapeuticstrategies may be developed. This book will be of vital interest tocell and molecular biologists at all levels, from graduate studentsto senior investigators, clinical researchers, and professionals inthe pharmaceutical and biotechnology industries.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1400
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Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 784
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Author: Dorothy Dianne Hodges
Publisher:
ISBN:
Category : Drosophila melanogaster
Languages : en
Pages : 558
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Book Description
Up to 480 isoforms of Drosophila muscle myosin heavy chain (MHC) can be generated by the process of alternative splicing. In order to better understand the regulation of MHC expression, we have analyzed the alternative splicing of MHC 3' end transcripts in vitro and in vivo. In Chapter 1 we describe the development and use of a Drosophila in-vitro splicing system to study the alternative splicing of penultimate exon 18. We demonstrate that pre-mRNA is spliced to exclude exon 18, as occurs in embryonic and larval muscle in vivo. However, when the 5' and 3' splice sites of exon 18 are modified to improve their binding to constitutive splicing factors, exon 18 is efficiently spliced to both flanking exons, as occurs in adult muscles in vivo. In Chapter 2 we express similarly modified transcripts in vivo using P element mediated germ line transformation. Mini-gene transcripts in which both splice sites of exon 18 are improved are now spliced to include exon 18 in larvae, as well as in adults. This is a complete splicing switch; all mRNAs typical of the normal larval splicing pattern have been eliminated. We also demonstrate that the correct 3' splice site of exon 18 is not utilized by the larval splicing machinery, even when the competing downstream 3' splice site is eliminated. Analysis of MHC [Delta] Int 17 mini-gene transcript splicing determined that intron 17 sequences are needed for intron 18 removal in larvae and adults. We also present results of cloning and sequencing the distantly related D. virilis MHC gene. Large stretches of non-coding sequences within exon 18 and a pyrimidine rich element in intron 17 are conserved between the D. virilis and D. melanogaster MHC genes. Mini-gene transcripts lacking most of the conserved exon 18 sequences were spliced in the correct stage-specific manner in vivo. However, analysis of splicing of mini-gene transcripts lacking the polypyrimidine sequence confirmed that it is essential for correct inclusion of exon 18 in adult mRNA, and suggests that binding of adult-specific, transacting factors to this element may mediate recognition and utilization of the weak 3' splice site of exon 18.
