Functional Intracellular Compartmentation of Gluconeogenesis, Glycolysis, Ethanol Oxidation and Urea Synthesis as Studied in the Perfused Rat Liver PDF Download
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Author: John William Kramer
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: John William Kramer
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: John William Kramer
Publisher:
ISBN:
Category :
Languages : en
Pages : 220
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Author:
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ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 978
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Author: Xerox University Microfilms
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 968
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Author:
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ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1276
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Author: University of California, Davis. Graduate Division
Publisher:
ISBN:
Category : Commencement ceremonies
Languages : en
Pages : 54
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Author:
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ISBN:
Category : Medicine
Languages : en
Pages : 928
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Author: Xerox University Microfilms
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1090
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Author: M. N. Berry
Publisher: Springer Science & Business Media
ISBN: 9780792361770
Category : Science
Languages : en
Pages : 630
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Book Description
It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309051266
Category : Science
Languages : en
Pages : 188
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Book Description
In the years since the third edition of this indispensable reference was published, a great deal has been learned about the nutritional requirements of common laboratory species: rat, mouse, guinea pig, hamster, gerbil, and vole. The Fourth Revised Edition presents the current expert understanding of the lipid, carbohydrate, protein, mineral, vitamin, and other nutritional needs of these animals. The extensive use of tables provides easy access to a wealth of comprehensive data and resource information. The volume also provides an expanded background discussion of general dietary considerations. In addition to a more user-friendly organization, new features in this edition include: A significantly expanded section on dietary requirements for rats, reporting substantial new findings. A new section on nutrients that are not required but that may produce beneficial results. New information on growth and reproductive performance among the most commonly used strains of rats and mice and on several hamster species. An expanded discussion of diet formulation and preparationâ€"including sample diets of both purified and natural ingredients. New information on mineral deficiency and toxicity, including warning signs. This authoritative resource will be important to researchers, laboratory technicians, and manufacturers of laboratory animal feed.
