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Author: Frances H. Arnold
Publisher: Humana Press
ISBN: 9781617374715
Category : Science
Languages : en
Pages : 0
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Book Description
Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks.
Author: Frances H. Arnold
Publisher: Humana Press
ISBN: 9781617374715
Category : Science
Languages : en
Pages : 0
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Book Description
Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks.
Author: Frances H. Arnold
Publisher: Springer Science & Business Media
ISBN: 1592593968
Category : Science
Languages : en
Pages : 381
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Book Description
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
Author: Stephanie Brody
Publisher: Routledge
ISBN: 1317551540
Category : Psychology
Languages : en
Pages : 319
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Book Description
2020 Gradiva Award Nominee, Best Edited Book Psychoanalytic Perspectives on Women and Their Experience of Desire, Ambition and Leadership considers how these factors can be understood, nurtured, or thwarted and the subsequent impact on women’s identity, authority and satisfaction. Psychoanalysis has long struggled with its ideas about women, about who they are, how to work with them, and how to respect and encourage what women want. This book argues that psychoanalytic theory and practice must evolve to maintain its relevance in a volatile landscape. Each section of the book begins with a chapter that reviews contemporary ideas regarding women, as well as psychoanalytic history, gender bias, and societal norms and deficits. Three composite clinical stories allow our distinguished contributors to discuss the contexts within which individual experience can be affected, and the role that clinical work may have to mobilize and advance passion and vitality. In their discussions, the interplay of clinical psychoanalysis, sociopolitical context, and understanding of gender, combine to offer a unique perspective, built on decades of scholarship, personal experience, and clinical expertise. Psychoanalytic Perspectives on Women and Their Experience of Desire, Ambition and Leadership will serve as a reference for all psychoanalysts and psychoanalytic psychotherapists as well as gender studies scholars interested in the progress of psychoanalytic theory regarding women in the 21st century. Contributors to this book include: Rosemary Balsam, Brenda Bauer, Andrea Celenza, Diane Elise, Adrienne Harris, Dorothy Holmes, Nancy Kulish, Vivian Pendar, Dionne Powell, and Arlene Richards.
Author: Susanne Brakmann
Publisher: Wiley-VCH
ISBN: 9783527304233
Category : Science
Languages : en
Pages : 0
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Book Description
Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!
Author: William Arnold
Publisher: Berkley
ISBN: 9780425054819
Category : Biography & Autobiography
Languages : en
Pages : 212
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 230
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Author: Francena H. Arnold
Publisher: Moody Publishers
ISBN: 1575676885
Category : Fiction
Languages : en
Pages : 269
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Book Description
Eleanor's secret love for Chad could mean losing her inheritance and giving up a life-long dream. Will she follow her own will, or make the hard choice to submit her life to Christ's leadership? Now available with a contemporary new look, Not My Will is a classic story of love, loss, and surrender, with more than 500,000 copies sold.
Author: Justin Glenn
Publisher: Savas Publishing
ISBN: 194066926X
Category : Reference
Languages : en
Pages : 1223
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Book Description
This is the initial volume of a comprehensive history that traces the “Presidential line” of the Washingtons. Volume one begins with the immigrant John Washington who settled in Westmoreland Co., Va., in 1657, married Anne Pope, and was the great-grandfather of President George Washington. This volume continues the story of John and Anne’s family for a total of seven generations, collecting over 5,000 direct descendants. Future volumes will trace eight more generations with a total of over 63,000 descendants. Although structured in a genealogical format for the sake of clarity, this is no bare bones genealogy but a true family history with over 1,200 detailed biographical narratives. These in turn strive to convey the greatness of the family that produced not only The Father of His Country but many others, great and humble, who struggled to build that country. The Washingtons includes the time-honored John Wright line which in recent years has been challenged largely on the basis of DNA evidence. Volumes one and two will form a set, with a cumulative bibliography appearing at the end of volume 2. Volume two will highlight the most notable descendants and spouses from the later volumes, including such luminaries as General George S. Patton, the author Shelby Foote, and the actor Lee Marvin. All of the volumes, now estimated at fourteen in all, are virtually complete and are scheduled for release over the course of the next year.
Author: Charlie Kunkel and Roy Evans
Publisher: AuthorHouse
ISBN: 1481739190
Category : Biography & Autobiography
Languages : en
Pages : 349
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Book Description
This book shares the stories of 65 Gohman ancestors who grew up next to the Mississippi River in Central Minnesota. They are the third-generation members of the Gohman family that immigrated from Lower Saxony, Germany, to the United States in 1843 and migrated from Cincinnati to Minnesota in 1855. The first and second generations are introduced briefly. The lives of the Third-Generation spanned a period from 1868 to 1991, an amazing 123 years. Generally engaged as farmers, they were diverse personalities who responded to life experiences in diverse ways. They lived through times of both great prosperity and deep poverty. They experienced two world wars and dramatically changing technology. This generation of the Gohman family thrived as they adapted to the changes in their lives from the horse and buggy times to the days of the jet plane.
Author:
Publisher: Turner Publishing Company
ISBN: 1563114747
Category :
Languages : en
Pages : 456
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Book Description
