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Author: George G. Guilbault
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 744
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Book Description
Author: George G. Guilbault
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 744
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Book Description
Author: Stephen G Schulman
Publisher: Elsevier
ISBN: 1483160998
Category : Science
Languages : en
Pages : 299
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Book Description
Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.
Author: Paula G. Coble
Publisher: Cambridge University Press
ISBN: 0521764610
Category : Science
Languages : en
Pages : 407
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Book Description
A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.
Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 146157658X
Category : Science
Languages : en
Pages : 487
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Book Description
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Author: George G. Guilbault
Publisher: CRC Press
ISBN: 1000147657
Category : Science
Languages : en
Pages : 918
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Book Description
This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.
Author: Laura Marcu
Publisher: CRC Press
ISBN: 1439861684
Category : Science
Languages : en
Pages : 554
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Book Description
During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia
Author: David M. Jameson
Publisher: Taylor & Francis
ISBN: 1439806055
Category : Science
Languages : en
Pages : 305
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Book Description
In color throughout, this text helps readers acquire a sound understanding of basic fluorescence theory and practice. It takes them through the history of important discoveries to the most current advances. The author introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The text includes references in each chapter, more than 250 figures, and the chemical structures of the most widely used fluorescent molecules.
Author: Georges G. Guilbault
Publisher:
ISBN:
Category :
Languages : fr
Pages : 697
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Book Description
Author: Ashutosh Sharma
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 200
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Book Description
An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.
Author: J.H. Richardson
Publisher: Elsevier
ISBN: 0323152856
Category : Science
Languages : en
Pages : 384
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Book Description
Systematic Materials Analysis focuses on the broad range of instrumental methods that brings new approaches to materials analysts to yield the desired information about a given material. This book explores the specific instruments that briefly outline the theories of operation. Organized into ten chapters, this volume starts with an overview of the analytical methods on the bases of specimen limitations and information desired, and then examines the use of flow charts encompassing the various instruments. This text then discusses the use of the charts, which present a complete listing of analytical instrumentation arranged so as to enable the selection of the best method for a given analytical task. Other chapters outline the theories of operation and describe the capability of the methods for quantitative and qualitative measurements of chemical composition, texture, and structure as applicable. This book is a valuable resource for materials analysts, engineers, biological scientists, laboratory administrators, and researchers.
