Fluorescence and Phosphorescence Spectroscopy PDF Download
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Author: Stephen G Schulman
Publisher: Elsevier
ISBN: 1483160998
Category : Science
Languages : en
Pages : 299
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Book Description
Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.
Author: Stephen G Schulman
Publisher: Elsevier
ISBN: 1483160998
Category : Science
Languages : en
Pages : 299
Get Book Here
Book Description
Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.
Author: David Rendell
Publisher: John Wiley & Sons
ISBN: 9788126517244
Category :
Languages : en
Pages : 444
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Book Description
This introduction to a major area of growth in analytical chemistry is designed for students seeking general background on the subject and for established chemists who want greater familiarity with the uses of fluorescence and phosphorescence techniques. The complicated aspects of spectroscopic theory is treated clearly and simply, providing a solid basis for understanding the analytical applications described later in the book. It outlines the instrumentation required in analysis, with block diagrams to clarify major concepts, and explains the quantitative aspects of photoluminescence. The remainder of the book surveys methods of both organic and inorganic analytes using natural flourescence or the formation of flourescent derivatives. It includes coverage of recent developments such as flouroimmunoassay and room temperature phosphorescence. Case studies give concrete examples of current work in the field.· Study Guide· Supporting Practical Work· Bibliography· Instrumentation· Quantitative Flourimetry· Photoluminescence Methods in Analysis· Further Applications of Photoluminescence· Self-Assessment Questions and Responses · Units of Measurement
Author: Bernard Valeur
Publisher: Springer Science & Business Media
ISBN: 364256853X
Category : Science
Languages : en
Pages : 496
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Book Description
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Author: B. Straughan
Publisher: Springer Science & Business Media
ISBN: 9400957416
Category : Science
Languages : en
Pages : 334
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Book Description
It is fifteen years since Walker and Straw wrote the first edition of 'Spectroscopy' and considerable developments have taken place during that time in all fields of this expanding subject. In atomic spectroscopy, for example, where the principles required in a student text have been laid down for many years, there have been advances in optical pumping and double resonance which cannot be neglected at undergraduate level. In addition, nuclear quadrupole resonance (n.q.f.) and far infrared spectroscopy now merit separate chapters while addtional chapters dealing with Mbssbauer spectroscopy, photoelectron spectroscopy and group theory are an essential requisite for any modern spectroscopy textbook. When the idea for a new edition of Spectroscopy was first discussed it quickly became clear that the task of revision would be an impossible one for two authors working alone. Consequently it was decided that the new edition be planned and co-ordinated by two editors who were to invite specialists, each of whom had experience of presenting their subject at an undergraduate level, to contribute a new chapter or to revise extensively an existing chapter. In this manner a proper perspective of each topic has been provided without any sacri fice of the essential character and unity of the first edition. The expansion of subject matter has necessitated the division of the complete work into three self contained volumes. Volume I includes atomic, n.m.f., n.q.f., e.s.r. and Mbssbauer spectroscopy.
Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 0387463127
Category : Science
Languages : en
Pages : 961
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Book Description
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
Author: Bernard Valeur
Publisher: John Wiley & Sons
ISBN: 3527328467
Category : Science
Languages : en
Pages : 595
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Book Description
This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
Author: Dmitri B Papkovsky
Publisher: Royal Society of Chemistry
ISBN: 1788014553
Category : Science
Languages : en
Pages : 384
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Book Description
Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.
Author: Paula G. Coble
Publisher: Cambridge University Press
ISBN: 0521764610
Category : Science
Languages : en
Pages : 407
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Book Description
A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.
Author: George G. Guilbault
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 688
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Book Description
Author: Ashutosh Sharma
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 200
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Book Description
An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.
