Flow Cytometry Protocols PDF Download
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Author: Teresa S. Hawley
Publisher: Springer Nature
ISBN: 107163738X
Category :
Languages : en
Pages : 460
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Book Description
Author: Teresa S. Hawley
Publisher: Springer Nature
ISBN: 107163738X
Category :
Languages : en
Pages : 460
Get Book Here
Book Description
Author: Christine Goetz
Publisher: Springer
ISBN: 3319980718
Category : Medical
Languages : en
Pages : 229
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Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Author: Larry A. Sklar
Publisher: Oxford University Press
ISBN: 0195183142
Category : Medical
Languages : en
Pages : 401
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Book Description
Shows how flow cytometry is integrated into modern biotechnology. This volume deals with issues of content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, and more.
Author: Larry A. Sklar
Publisher: Oxford University Press
ISBN: 0198034784
Category : Science
Languages : en
Pages : 401
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Book Description
Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.
Author: Ewa M. Goldys
Publisher: John Wiley & Sons
ISBN: 0470083700
Category : Science
Languages : en
Pages : 398
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Book Description
A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.
Author: Howard M. Shapiro
Publisher: John Wiley & Sons
ISBN: 0471434035
Category : Science
Languages : en
Pages : 736
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Book Description
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Author: Natasha S. Barteneva
Publisher: Humana
ISBN: 9781493933006
Category : Medical
Languages : en
Pages : 0
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Book Description
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Author: Martin G. Wilkinson
Publisher: Caister Academic Press Limited
ISBN: 9781910190111
Category : Medical
Languages : en
Pages : 0
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Book Description
Flow cytometry is a powerful technique for the rapid analysis of single cells in a mixture. In microbiology, flow cytometry permits the reliable and rapid detection of single or multiple microbes and can provide information about their distribution within cell populations. Flow cytometry may also lead to a faster means of viability counting of microorganisms while, at the same time, enabling a better understanding of all bacterial cells within a given population. Specially adapted commercial cytometers for microbial detection are being developed. In this text, renowned contributors have brought together a wealth of experience demonstrating the power and limitations of flow cytometry as it currently stands in the field of microbiology. The book commences with an overview of flow cytometry by Professor Howard Shapiro, one of the most eminent scientists in the area of flow cytometry. Further chapters discuss: cytometry technology and applications in environmental biotechnology * microbial community fingerprinting * clinical microbiology * lactic acid bacteria * spore forming bacteria * yeasts and fungi * water analysis * chip-based cytometry assays. The final chapter describes the adoption of flow cytometry to routine water quality analysis in Switzerland and provides a fascinating case study of how this emerging microbial technology overcame technical, regulatory, and practical issues to become a standard rapid quality control methodology. The book provides a thorough description of flow cytometry and includes practical and up-to-date information aimed specifically at microbiologists. *** Librarians: ebook available on ProQuest and EBSCO [Subject: Microbiology, Life Science]
Author: Virginia Litwin
Publisher: John Wiley & Sons
ISBN: 0470922788
Category : Medical
Languages : en
Pages : 404
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Book Description
This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry. The second section focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. The third section contains a single chapter presenting an in depth discussion of validation considerations and regulatory compliance issues associated with drug development.
Author: Hong-Hwa Chen
Publisher: World Scientific
ISBN: 981432793X
Category : Gardening
Languages : en
Pages : 380
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Book Description
Orchid Biotechnology II presents a series of recent works on both basic and applied researches in biotechnology progress for Phalaenopsis and Oncidium orchids. These include the development of flower, ovule, gynostemium and perianth, the discovery of new orchid-infecting viruses and virus movement, secondary metabolites, technology of DNA endoduplication and genetic transformation, growth regulation by micronutrition and orchid mycorrhiza, and plant growth substances for flowering. The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially in the past ten years worldwide. This book focuses on the recent advances in the research of orchid biotechnology from the past ten years in Taiwan. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve flower quality and breeding of new varieties.
