Flow Cytometry Basics for the Non-Expert PDF Download
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Author: Christine Goetz
Publisher: Springer
ISBN: 3319980718
Category : Medical
Languages : en
Pages : 229
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Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Author: Christine Goetz
Publisher: Springer
ISBN: 3319980718
Category : Medical
Languages : en
Pages : 229
Get Book Here
Book Description
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.
Author: Alice Longobardi Givan
Publisher: John Wiley & Sons
ISBN: 1118688392
Category : Science
Languages : en
Pages : 309
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Book Description
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Author: Marion G. Macey
Publisher: Springer Science & Business Media
ISBN: 1597454516
Category : Medical
Languages : en
Pages : 289
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Book Description
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Author: Howard M. Shapiro
Publisher: John Wiley & Sons
ISBN: 0471434035
Category : Science
Languages : en
Pages : 736
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Book Description
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Author: Atul Tiwari
Publisher: John Wiley & Sons
ISBN: 1119084490
Category : Technology & Engineering
Languages : en
Pages : 704
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Book Description
Research in the area of nanoindentation has gained significant momentum in recent years, but there are very few books currently available which can educate researchers on the application aspects of this technique in various areas of materials science. Applied Nanoindentation in Advanced Materials addresses this need and is a comprehensive, self-contained reference covering applied aspects of nanoindentation in advanced materials. With contributions from leading researchers in the field, this book is divided into three parts. Part one covers innovations and analysis, and parts two and three examine the application and evaluation of soft and ceramic-like materials respectively. Key features: A one stop solution for scholars and researchers to learn applied aspects of nanoindentation Contains contributions from leading researchers in the field Includes the analysis of key properties that can be studied using the nanoindentation technique Covers recent innovations Includes worked examples Applied Nanoindentation in Advanced Materials is an ideal reference for researchers and practitioners working in the areas of nanotechnology and nanomechanics, and is also a useful source of information for graduate students in mechanical and materials engineering, and chemistry. This book also contains a wealth of information for scientists and engineers interested in mathematical modelling and simulations related to nanoindentation testing and analysis.
Author: Dale Ensminger
Publisher: CRC Press
ISBN: 0824758897
Category : Science
Languages : en
Pages : 768
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Book Description
Recent advances in power electronics greatly benefit the multidisciplinary field of modern ultrasonics. More powerful, compact, and versatile electronic chips and software enable new computer-based devices for real-time data capture, storage, analysis, and display and advance the science and technology employed in commercial systems and applications of ultrasound. Reviewing the scientific basis behind these improvements, Ultrasonics: Fundamentals, Technologies, and Applications, Third Edition discusses them in detail, with new and additional figures and references, offering a completely revised and expanded examination of the state of modern ultrasonics. This new edition of a bestselling industry reference discusses the full breadth of ultrasonics applications for industrial and medical use and provides the fundamentals and insights gathered over the authors’ collective 80 years in the field. It provides a unique and comprehensive treatment of the science and technology behind the latest advancements and applications in both low and high power implementations. Coverage combines fundamental physics, a review and analysis of sensors and transducers, and the systems required for the full spectrum of industrial, nondestructive testing and medical and biomedical uses. It includes citations of numerous references and covers both main stream and the more unusual and obscure applications of ultrasound. Ultrasonics is ubiquitous in its industrial applications for sensing, NDT, and process measurements, in high power forms for processing and sonochemistry, as well as in medical procedures where it is used for diagnosis, therapy and surgery. This book provides a complete overview of the field, presenting numerous applications, cutting-edge advancements and improvements, additional figures and references, and a look at future directions.
Author: Alexander E. Kalyuzhny
Publisher: Springer
ISBN: 3319308939
Category : Science
Languages : en
Pages : 91
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Book Description
Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.
Author: Leanne Vogel
Publisher: Victory Belt Publishing
ISBN: 1628603704
Category : Cooking
Languages : en
Pages : 416
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Book Description
International bestselling author, Leanne Vogel delivers the ultimate resource guide for women looking to take charge of their health through a fat-fueled, ketogenic diet. Leanne draws upon her many years of experience as a Nutrition Educator as well as her personal success to bring women this first-ever custom built nutrition program that will meet their individual needs. Keto for Women delves into what the keto diet is (and is not) and how women can reap the many health benefits by using a targeted method specifically designed for their bodies. Throughout her book, Leanne teaches women how hormone imbalances cause many of the negative symptoms they experience and what keto foods and protocols will work best to remedy those. By teaching women how to understand their bodies, they will be empowered to find solutions that are right for them as individuals and stay in control each step of the way. Leanne sheds new light on the role nutrition plays in: thyroid imbalances, menopause, PCOS, adrenal fatigue, pregnancy, menstrual cycles, sex drive and more. She also details the positive effects of the keto diet on emotional and mental well-being giving women a path to total health, mind, body and soul. Keto for Women provides customizable resources with ingredients that target and balance specific health problems. Leanne also offers maps that you can follow to develop personalized meals that are based upon the ingredients chosen for your unique needs. Keto for Women complements Leanne’s 12-week program (happyketobody.com) that has helped more than 3,000 women take charge of their health, using a customized ketogenic diet to balance their bodies.
Author: Nader Rifai
Publisher: Elsevier Health Sciences
ISBN: 0323834671
Category : Medical
Languages : en
Pages : 4232
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Book Description
Use THE definitive reference for laboratory medicine and clinical pathology! Tietz Textbook of Laboratory Medicine, 7th Edition provides the guidance necessary to select, perform, and evaluate the results of new and established laboratory tests. Comprehensive coverage includes the latest advances in topics such as clinical chemistry, genetic metabolic disorders, molecular diagnostics, hematology and coagulation, clinical microbiology, transfusion medicine, and clinical immunology. From a team of expert contributors led by Nader Rifai, this reference includes access to wide-ranging online resources on Expert Consult — featuring the comprehensive product with fully searchable text, regular content updates, animations, podcasts, over 1300 clinical case studies, lecture series, and more. - Authoritative, current content helps you perform tests in a cost-effective, timely, and efficient manner; provides expertise in managing clinical laboratory needs; and shows how to be responsive to an ever-changing environment. - Current guidelines help you select, perform, and evaluate the results of new and established laboratory tests. - Expert, internationally recognized chapter authors present guidelines representing different practices and points of view. - Analytical criteria focus on the medical usefulness of laboratory procedures. - Use of standard and international units of measure makes this text appropriate for any user, anywhere in the world. - Elsevier eBooks+ provides the entire text as a fully searchable eBook, and includes animations, podcasts, more than 1300 clinical case studies, over 2500 multiple-choice questions, a lecture series, and more, all included with print purchase. - NEW! 19 additional chapters highlight various specialties throughout laboratory medicine. - NEW! Updated, peer-reviewed content provides the most current information possible. - NEW! The largest-ever compilation of clinical cases in laboratory medicine is included with print purchase on Elsevier eBooks+. - NEW! Over 100 adaptive learning courses included with print purchase on Elsevier eBooks+ offer the opportunity for personalized education.
Author: Niclas Thomas
Publisher:
ISBN: 9781985665675
Category :
Languages : en
Pages : 158
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Book Description
Data science is a complex subject, but nevertheless one that can be made accessible to all through clear, intuitive explanations and worked examples. Existing software that forms the backbone of an immunologist's analytical toolkit (such as FlowJo and Prism) are expensive, inflexible and promotes a narrow mindset when it comes to analysing your data. On the other hand, the Python and R programming languages are open source, free and entirely customisable, giving the user the ability to implement any analysis they wish. Although programming languages can seem daunting to the uninitiated, it's far easier to learn than many immunologists may think. Rather than seeking to become an expert programmer, an understanding of the main concepts is more than enough to conduct your own bespoke analyses when coupled with a sound mathematical and statistical understanding. Our new book focusses on the practical aspects of data science, providing sufficient theoretical background without delving into all of the details of each of the methods presented. Introductory chapters are presented alongside the analysis of a publicly available data set, allowing the reader to have practical hands-on experience when learning about important concepts in statistics, machine learning and programming. Topics include: - How to build a predictive model How to visualise high-dimensional data Basics of programming in Python and R What techniques exist to cluster data Which statistics test to use/why/when What is dimension reduction; when and how to use it Once these fundamental topics have been covered, a number of case studies are presented, along with the underlying data, accompanying code and full explanations on topics such as automated, data-driven flow cytometry, building predictive models of disease using gene expression profiling and analysing high throughput sequencing data.
