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Languages : en
Pages : 174
Book Description
The ability to form embryogenic cultures and regenerate green plants in vitro is a critical factor in plant genetic engineering, plant propagation and transformation-based research. Maize regeneration ability is genotype-dependent and limiting to tissue culture-based methodologies. The overall goal of this research is to further our understanding of tissue culture response in maize. A backcross-derived mapping population between the highly embryogenic and regenerable maize genotype, A188 was crossed to the poorly culturable maize reference inbred line B73. Near-isogenic lines were screened for tissue culture response. High-resolution mapping in this material to further refine the position of genes on chromosome 3 conferring culture response resulted in two flanking single nucleotide polymorphic markers with a physical distance between markers of 3,053kb or a genetic distance of 4 centimorgans, based on recombination frequency in segregating F2 individuals (n=2243). A fine-mapping experiment revealed highly significant marker-trait associations (P
