Exploring Fennoscandian agricultural history through genetic analysis of aged crop materials

Exploring Fennoscandian agricultural history through genetic analysis of aged crop materials PDF Author: Maria Lundström
Publisher: Linköping University Electronic Press
ISBN: 917685194X
Category :
Languages : en
Pages : 59

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Book Description
Crop plants have undergone a multitude of genetic changes during and following their domestication. The spread of agriculture brought the crops to new geographic regions exposing them to new environments and selection pressures along the way. This gave rise to many local variants with traits favoured both by agricultural practices and the environment. Agriculture was introduced in Fennoscandia (Norway, Sweden, Finland and Denmark) around 4000 BC. The composition of the archaeobotanical record gives some clues as to which species were cultivated, but macroscale analyses rarely reach beyond that. Therefore, methods like genetic analysis are necessary to expand our knowledge about the history of crop cultivation. Under optimal conditions, DNA can survive in biological samples for several hundred thousand years. The preservation of plant specimens in the Fennoscandian climate has, however, rarely been explored. This thesis therefore attempts to dive deeper into the Fennoscandian cultivation history through genetic analyses of aged plant materials from both museum collections and archaeological sources. Cereal grains from a range of preservation conditions were evaluated to find which ones might be of interest for genetic investigations. Desiccated materials gave the highest success rates, in agreement with previous studies. Waterlogged materials appeared to contain small amounts of endogenous DNA, whereas genetic analysis of charred cereals failed completely in all samples. Population structure was investigated in 17-19th century materials of both barley and rye from Sweden and Finland. Northern and southern populations of Finnish six-row barley were distinct from one another. In southern Sweden, genetic analysis suggested conserved population structure extending over 200 years. The genetic composition of rye also seemed mostly conserved, but rye did not show geographic population structure across the investigated region in Sweden and Finland. A long-standing question in Fennoscandian crop history has been the interpretation of historical written records mentioning Brassica (cole crops, turnips and mustards), as well as the species identity of archaeobotanical finds of Brassica seeds. Thus, Next Generation Sequencing (NGS) was applied to identify which Brassica types that were cultivated in 17th century Kalmar, Sweden. The analysis corroborated morphological species classification in two of the investigated subfossil seeds, whereas no conclusions could be drawn from the remaining samples. The genome coverages were too low to allow subspecies identification. Wheat has been cultivated in Fennoscandia since the introduction of agriculture but has increased dramatically in importance over the last century. The functional allele of the wheat nutrition gene NAM-B1 was found to be particularly prominent in Fennoscandian wheats, likely associated with its effect on grain maturation time. Here the evolutionary history of NAM-B1 was investigated to see if it could truly be considered a domestication gene as suggested in a previous study. By studying extant landrace materials of Mediterranean tetraploid wheat, it was found that the non-functional allele showed signs indicative of a selective sweep. This selection did not, however, appear to have occurred during domestication. In conclusion, aged plant specimens from both museum and archaeological contexts could contribute greatly to our knowledge about historical cultivation, extending the investigated period into the mid 17th century. Subfossil and waterlogged archaeobotanical materials do contain endogenous DNA, suggesting that they are better suited for genetic analysis than charred ones, at least as far as cereals are concerned. There is potential for classifying archaeological Brassica remains using NGS, even though further optimisation of sample and library preparation may be necessary. And finally, despite NAM-B1 showing signs of selection it should not be considered a domestication gene in tetraploid wheat.

Exploring Fennoscandian agricultural history through genetic analysis of aged crop materials

Exploring Fennoscandian agricultural history through genetic analysis of aged crop materials PDF Author: Maria Lundström
Publisher: Linköping University Electronic Press
ISBN: 917685194X
Category :
Languages : en
Pages : 59

Get Book Here

Book Description
Crop plants have undergone a multitude of genetic changes during and following their domestication. The spread of agriculture brought the crops to new geographic regions exposing them to new environments and selection pressures along the way. This gave rise to many local variants with traits favoured both by agricultural practices and the environment. Agriculture was introduced in Fennoscandia (Norway, Sweden, Finland and Denmark) around 4000 BC. The composition of the archaeobotanical record gives some clues as to which species were cultivated, but macroscale analyses rarely reach beyond that. Therefore, methods like genetic analysis are necessary to expand our knowledge about the history of crop cultivation. Under optimal conditions, DNA can survive in biological samples for several hundred thousand years. The preservation of plant specimens in the Fennoscandian climate has, however, rarely been explored. This thesis therefore attempts to dive deeper into the Fennoscandian cultivation history through genetic analyses of aged plant materials from both museum collections and archaeological sources. Cereal grains from a range of preservation conditions were evaluated to find which ones might be of interest for genetic investigations. Desiccated materials gave the highest success rates, in agreement with previous studies. Waterlogged materials appeared to contain small amounts of endogenous DNA, whereas genetic analysis of charred cereals failed completely in all samples. Population structure was investigated in 17-19th century materials of both barley and rye from Sweden and Finland. Northern and southern populations of Finnish six-row barley were distinct from one another. In southern Sweden, genetic analysis suggested conserved population structure extending over 200 years. The genetic composition of rye also seemed mostly conserved, but rye did not show geographic population structure across the investigated region in Sweden and Finland. A long-standing question in Fennoscandian crop history has been the interpretation of historical written records mentioning Brassica (cole crops, turnips and mustards), as well as the species identity of archaeobotanical finds of Brassica seeds. Thus, Next Generation Sequencing (NGS) was applied to identify which Brassica types that were cultivated in 17th century Kalmar, Sweden. The analysis corroborated morphological species classification in two of the investigated subfossil seeds, whereas no conclusions could be drawn from the remaining samples. The genome coverages were too low to allow subspecies identification. Wheat has been cultivated in Fennoscandia since the introduction of agriculture but has increased dramatically in importance over the last century. The functional allele of the wheat nutrition gene NAM-B1 was found to be particularly prominent in Fennoscandian wheats, likely associated with its effect on grain maturation time. Here the evolutionary history of NAM-B1 was investigated to see if it could truly be considered a domestication gene as suggested in a previous study. By studying extant landrace materials of Mediterranean tetraploid wheat, it was found that the non-functional allele showed signs indicative of a selective sweep. This selection did not, however, appear to have occurred during domestication. In conclusion, aged plant specimens from both museum and archaeological contexts could contribute greatly to our knowledge about historical cultivation, extending the investigated period into the mid 17th century. Subfossil and waterlogged archaeobotanical materials do contain endogenous DNA, suggesting that they are better suited for genetic analysis than charred ones, at least as far as cereals are concerned. There is potential for classifying archaeological Brassica remains using NGS, even though further optimisation of sample and library preparation may be necessary. And finally, despite NAM-B1 showing signs of selection it should not be considered a domestication gene in tetraploid wheat.

Dog behaviour

Dog behaviour PDF Author: Ann-Sofie Sundman
Publisher: Linköping University Electronic Press
ISBN: 9176850722
Category :
Languages : en
Pages : 67

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Book Description
Dogs, Canis familiaris, share the lives of humans all over the world. That dogs, and the behavior of dogs, are of interest to many is therefore no surprise. In this thesis, the main aim has been to identify factors that affect dogs’ behaviours. The dog, Canis familiaris, is our first domesticated animal. Since domestication, various types of dogs have developed through adaptation to an environment shared with humans and through our selective breeding, resulting in a unique variation in morphology and behaviour. Although there is an individual variation in the behaviour of dogs, there is also a difference between breeds. Moreover, selection during the last decades has split some breeds into divergent types. Labrador and golden retrievers are divided into a common type, for show and companionship, and a field type, for hunting. By comparing the breed types, we can study the effects of recent selection. In Paper I, we investigate differences in general behavioural traits between Labrador and golden retriever and between common and field type within the two breeds by using results from the standardized behaviour test Dog Mentality Assessment. There were differences between breeds and types for all behavioural traits. However, there was also an interaction between breed and type. Thus, a common/field-type Labrador does not behave like a common/field-type golden retriever. Even though they have been selected for similar traits, the selection has affected the general behavioural traits differently in the two breeds. In paper II, we were interested in dogs’ human-directed social skills. Dogs have a high social competence when it comes to humans. Two experiments commonly used to study these skills are the problem-solving test, where dogs’ human-directed behaviours when faced with a problem are measured, and the pointing test, where dogs are tested on how well they understand human gestures. We compared the social skills of German shepherds and Labrador retrievers, and of common- and field-type Labradors. Labradors were more successful in the pointing test and German shepherds stayed closer to their owners during the problem solving. Among Labrador types, the field type had more human eye contact than the common type. Importantly, when comparing the two experiments, we found no positive correlations between the problem-solving test and the pointing test, suggesting that the two tests measure different aspects of human-directed social behaviour in dogs. A previous study has identified two suggestive genetic regions for human-directed social behaviours during the problem-solving test in beagles. In paper III, we show that these SNPs are also associated to social behaviours in Labrador and golden retrievers. Moreover, the Labrador breed types differed significantly in allele frequencies. This indicates that the two SNPs have been affected by recent selection and may have a part in the differences in sociability between common and field type. The behaviour of dogs cannot simply be explained by genetics, there is also an environmental component. In paper IV, we study which factors that affect long-term stress in dogs. Long-term cortisol can be measured by hair samples. We found a clear synchronization in hair cortisol concentrations between dogs and their owners. Neither dogs’ activity levels nor their behavioural traits affected the cortisol, however, the personality of the owners did. Therefore, we suggest that dogs mirror the stress level of their owners. The mediator between genes and the environment is epigenetics, and one epigenetic factor is DNA methylation. In paper V, we compared methylation patterns of wolves and dogs as well as dog breeds. Between both wolves and dogs and among dogs there were substantial differences in methylated DNA regions, suggesting that DNA methylation is likely to contribute to the vast variation among canines. We hypothesize that epigenetic factors have been important during domestication and in breed formation. In this thesis, I cover several aspects on how dogs’ behaviours can be affected, and paint an intricate picture on how genetics, epigenetics, and human-dog relations forms dog behaviour.

The Sepik-Ramu

The Sepik-Ramu PDF Author: Pamela Swadling
Publisher:
ISBN:
Category : East Sepik Province (Papua New Guinea)
Languages : en
Pages : 80

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Book Description


The Regional Impacts of Climate Change

The Regional Impacts of Climate Change PDF Author: Intergovernmental Panel on Climate Change. Working Group II.
Publisher: Cambridge University Press
ISBN: 9780521634557
Category : Science
Languages : en
Pages : 532

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Book Description
Cambridge, UK : Cambridge University Press, 1998.

The Ocean and Cryosphere in a Changing Climate

The Ocean and Cryosphere in a Changing Climate PDF Author: Intergovernmental Panel on Climate Change (IPCC)
Publisher: Cambridge University Press
ISBN: 1009178466
Category : Science
Languages : en
Pages : 1807

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Book Description
The Intergovernmental Panel on Climate Change (IPCC) is the leading international body for assessing the science related to climate change. It provides policymakers with regular assessments of the scientific basis of human-induced climate change, its impacts and future risks, and options for adaptation and mitigation. This IPCC Special Report on the Ocean and Cryosphere in a Changing Climate is the most comprehensive and up-to-date assessment of the observed and projected changes to the ocean and cryosphere and their associated impacts and risks, with a focus on resilience, risk management response options, and adaptation measures, considering both their potential and limitations. It brings together knowledge on physical and biogeochemical changes, the interplay with ecosystem changes, and the implications for human communities. It serves policymakers, decision makers, stakeholders, and all interested parties with unbiased, up-to-date, policy-relevant information. This title is also available as Open Access on Cambridge Core.

The Oxford Handbook of the Archaeology and Anthropology of Hunter-Gatherers

The Oxford Handbook of the Archaeology and Anthropology of Hunter-Gatherers PDF Author: Vicki Cummings
Publisher: OUP Oxford
ISBN: 0191025275
Category : Social Science
Languages : en
Pages : 1361

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Book Description
For more than a century, the study of hunting and gathering societies has been central to the development of both archaeology and anthropology as academic disciplines, and has also generated widespread public interest and debate. The Oxford Handbook of the Archaeology and Anthropology of Hunter-Gatherers provides a comprehensive review of hunter-gatherer studies to date, including critical engagements with older debates, new theoretical perspectives, and renewed obligations for greater engagement between researchers and indigenous communities. Chapters provide in-depth archaeological, historical, and anthropological case-studies, and examine far-reaching questions about human social relations, attitudes to technology, ecology, and management of resources and the environment, as well as issues of diet, health, and gender relations - all central topics in hunter-gatherer research, but also themes that have great relevance for modern global society and its future challenges. The Handbook also provides a strategic vision for how the integration of new methods, approaches, and study regions can ensure that future research into the archaeology and anthropology of hunter-gatherers will continue to deliver penetrating insights into the factors that underlie all human diversity.

Limnogeology: Progress, Challenges and Opportunities

Limnogeology: Progress, Challenges and Opportunities PDF Author: Michael R. Rosen
Publisher: Springer Nature
ISBN: 3030665763
Category : Science
Languages : en
Pages : 592

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Book Description
This book honors the career of Professor Elizabeth Gierlowski-Kordesch who was a pioneer and leader in the field of limnogeology since the 1980s. Her work was instrumental in guiding students and professionals in the field until her untimely death in 2016. This collection of chapters was written by her colleagues and students and recognize the important role that Professor Gierlowski-Kordesch had in advancing the field of limnogeology. The chapters show the breadth of her reach as these have been contributed from virtually every continent. This book will be a primary reference for scientists, professionals and graduate students who are interested in the latest advances in limnogeologic processes and basin descriptions in North and South America, Europe, Africa, and China. *Free supplementary material available online for chapters 3,11,12 and 13. Access by searching for the book on link.springer.com

Fundamentals of Geomorphology

Fundamentals of Geomorphology PDF Author: Richard John Huggett
Publisher: Routledge
ISBN: 1135281130
Category : Science
Languages : en
Pages : 909

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Book Description
This extensively revised, restructured, and updated edition continues to present an engaging and comprehensive introduction to the subject, exploring the world’s landforms from a broad systems perspective. It covers the basics of Earth surface forms and processes, while reflecting on the latest developments in the field. Fundamentals of Geomorphology begins with a consideration of the nature of geomorphology, process and form, history, and geomorphic systems, and moves on to discuss: structure: structural landforms associated with plate tectonics and those associated with volcanoes, impact craters, and folds, faults, and joints process and form: landforms resulting from, or influenced by, the exogenic agencies of weathering, running water, flowing ice and meltwater, ground ice and frost, the wind, and the sea; landforms developed on limestone; and landscape evolution, a discussion of ancient landforms, including palaeosurfaces, stagnant landscape features, and evolutionary aspects of landscape change. This third edition has been fully updated to include a clearer initial explanation of the nature of geomorphology, of land surface process and form, and of land-surface change over different timescales. The text has been restructured to incorporate information on geomorphic materials and processes at more suitable points in the book. Finally, historical geomorphology has been integrated throughout the text to reflect the importance of history in all aspects of geomorphology. Fundamentals of Geomorphology provides a stimulating and innovative perspective on the key topics and debates within the field of geomorphology. Written in an accessible and lively manner, it includes guides to further reading, chapter summaries, and an extensive glossary of key terms. The book is also illustrated throughout with over 200 informative diagrams and attractive photographs, all in colour.

Allelopathy

Allelopathy PDF Author: Manuel Joaquín Reigosa Roger
Publisher: Springer Science & Business Media
ISBN: 9781402042799
Category : Nature
Languages : en
Pages : 660

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Book Description
This book provides the reader relevant information about actual knowledge about the process of allelopathy, covering all aspects from the molecular to the ecological level. Special relevance is given to the physiological and ecophysiological aspects of allelopathy. Several ecosystems are studied and methodological considerations are taken into account in several different chapters. The book has been written to be useful both for Ph.D. students and for senior researchers, so the chapters include all necessary information to be read by beginners, but they also include a lot of useful information and discussion for the initiated.

Physicochemical and Environmental Plant Physiology

Physicochemical and Environmental Plant Physiology PDF Author: Park S. Nobel
Publisher: Academic Press
ISBN: 032313761X
Category : Science
Languages : en
Pages : 656

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Book Description
This text is the successor volume to Biophysical Plant Physiology and Ecology (W.H. Freeman, 1983). The content has been extensively updated based on the growing quantity and quality of plant research, including cell growth and water relations, membrane channels, mechanisms of active transport, and the bioenergetics of chloroplasts and mitochondria. One-third of the figures are new or modified, over 190 new references are incorporated, the appendixes on constants and conversion factors have doubled the number of entries, and the solutions to problems are given for the first time. Many other changes have emanated from the best laboratory for any book, the classroom.· Covers water relations and ion transport for plant cells; diffusion, chemical potential gradients, solute movement in and out of plant cells· Covers interconnection of various energy forms; light, chlorophyll and accessory photosynthesis pigments, ATP and NADPH· Covers forms in which energy and matter enter and leave a plant; energy budget analysis, water vapor and carbon dioxide, water movement from soil to plant to atmosphere