Expanding the Scope of the Enzymatic Synthesis of DNA for PDF Download

Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Expanding the Scope of the Enzymatic Synthesis of DNA for PDF full book. Access full book title Expanding the Scope of the Enzymatic Synthesis of DNA for by Aaron M. Leconte. Download full books in PDF and EPUB format.

Expanding the Scope of the Enzymatic Synthesis of DNA for

Expanding the Scope of the Enzymatic Synthesis of DNA for PDF Author: Aaron M. Leconte
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 414

Get Book Here

Book Description
DNA polymerases synthesize DNA, the essential biomolecule responsible for encoding the complex information necessary for life, with remarkable efficiency and fidelity. Chemists and biologists have exploited this extraordinary enzyme to develop a wide range of biotechnological tools, including but certainly not limited to PCR and Sanger sequencing. While these landmark applications continue to be relied upon to this day, they represent only a small fraction of the possible uses of these enzymes. Here, I detail my work to expand the scope of the enzymatic synthesis of DNA in two distinct fields. Chapters 2-5 detail efforts to identify replicable candidate unnatural base pairs to expand the genetic alphabet from the natural four base code to an expanded six base code. Using a wide range of techniques, including chemical synthesis, directed evolution of DNA polymerases, and screening based methodologies, the d 5SICS:d MMO2 base pair is identified, resulting in our strongest candidate base pair identified to date. Chapter 6 details the use of an activity based phage display system to identify a DNA polymerase that possesses improved recognition of substrates applicable in next generation sequencing applications.