Evaluation of Agronomic, Seed Quality Traits, and Transcript Abundance in Conventional High Oleic Soybeans with Mutant FAD2-1A and FAD2-1B Genes

Evaluation of Agronomic, Seed Quality Traits, and Transcript Abundance in Conventional High Oleic Soybeans with Mutant FAD2-1A and FAD2-1B Genes PDF Author: Lauren Kathryn Richardson
Publisher:
ISBN:
Category : Linoleic acid
Languages : en
Pages : 85

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Book Description
Two soybean omega-6 fatty acid desaturase genes [FAD2-1A and FAD2- 1B] are responsible for converting oleic acid into linoleic acid (Okuley et al., 1994). Plant introductions [PI] 603452 and 283327 have naturally occurring mutations in FAD2-1A and FAD2-1B, respectively, which hinder the conversion of oleic acid to polyunsaturated fatty acids. The end result is more functional soybean oil with exceptionally high levels of oleic acid. The homozygous alleles of FAD2-1A and FAD2-1B were combined in a BC3F2:4 [backcross three, second filial generation selected, advanced to the fourth filial generation] population of forty-eight lines and were evaluated in Tennessee. Each line belonged to a double homozygous genotypic class: i) double wild-type [WT] (AABB), ii) mutant FAD2-1A with WT FAD2-1B (aaBB), iii) the converse (AAbb), and iv) double mutant (aabb). All genotypic groups yielded similarly to each other and to three high yielding checks (p