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Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN: 3527338802
Category : Science
Languages : en
Pages : 470
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Book Description
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN: 3527338802
Category : Science
Languages : en
Pages : 470
Get Book Here
Book Description
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
Author: Reiner Westermeier
Publisher: Wiley-Blackwell
ISBN:
Category : Science
Languages : en
Pages : 364
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Book Description
Electrophoresis; Isotachophoresis; Bloting; Intrumentation; Interpretation of electropherograms; Equipment for section II; Methods; Page of dyes; Agarose and immunoelectrophoresis; Tiration curve analysis; Native PAGE in amphoteric buffers; Agarose IEF; PAGIEF in rehydrated gels; SDS-polyacrylamide electrophoresis; Semi-dry blotting of proteins; IEE in immobilized pH gradients; High resolution 2D eletrophoresis; PAGE of double stranded DNA; Native PAGE of single stranded DNA; Denaturing gradient gel electrophoresis; Denaturing PAGE of DNA; Vertical PAGE; A trouble-shooting guide.
Author: Reinhard Kuhn
Publisher: Springer Science & Business Media
ISBN: 364278058X
Category : Science
Languages : en
Pages : 381
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Book Description
Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading.
Author: Robert Weinberger
Publisher: Academic Press
ISBN:
Category : Science
Languages : en
Pages : 488
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Book Description
Capillary zone electrophoresis : basic concepts -- Capillary zone electrophoresis : methods development -- Capillary zone electrophoresis : secondary equilibrium, micelles, cyclodextrins, and related reagents -- Capillary isoelectric focusing -- Size separations in capillary gels and polymer networks -- Capillary electrochromatography -- Injection -- Detection -- Putting it all together.
Author: Bruce Birren
Publisher: Academic Press
ISBN: 0323137628
Category : Science
Languages : en
Pages : 272
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Book Description
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. - Reviews all types of pulsed field gel electrophoresis - Describes all commercially available systems and summarizes advantages and limitations of each - Includes step-by-step protocols for sample preparation and analysis - Illustrated with photographs that depict - How to run gels: What the results should look like - What they look like when they go wrong - Covers applications to a wide range of organisms - Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism
Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN:
Category : Medical
Languages : en
Pages : 316
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Book Description
Author: Reiner Westermeier
Publisher: John Wiley & Sons
ISBN: 3527604448
Category : Science
Languages : en
Pages : 426
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Book Description
This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
Author: T. Rabilloud
Publisher: Springer Science & Business Media
ISBN: 9783540657927
Category : Science
Languages : en
Pages : 272
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Book Description
With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.
Author: Reiner Westermeier
Publisher: Wiley-Blackwell
ISBN: 9783527300129
Category : Science
Languages : en
Pages : 278
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Book Description
A comprehensive compilation of methods for all variations of modern electrophoresis. In-depth presentation of the physical-chemical background is followed by numerous practical tips based on application-oriented examples. Both newcomers and experts will appreciate the detailed instructions and the carefully worked-out problems that characterize this book. Reiner Westermeier has become well-known in the field of electrophoresis through his numerous publications, seminars and lectures.
Author: Patrick Camilleri
Publisher: CRC Press
ISBN: 9780849391279
Category : Medical
Languages : en
Pages : 586
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Book Description
The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
