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Author: Lawrence Berliner
Publisher: Springer Science & Business Media
ISBN: 0306465337
Category : Medical
Languages : en
Pages : 623
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Book Description
Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.
Author: Lawrence Berliner
Publisher: Springer Science & Business Media
ISBN: 0306465337
Category : Medical
Languages : en
Pages : 623
Get Book Here
Book Description
Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.
Author: Lawrence J. Berliner
Publisher: Springer Science & Business Media
ISBN: 0306471094
Category : Medical
Languages : en
Pages : 622
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Book Description
Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.
Author: Lawrence Berliner
Publisher: Springer Science & Business Media
ISBN: 1461565340
Category : Science
Languages : en
Pages : 354
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Book Description
Biological magnetic resonance (NMR and EPR) is a rapidly expanding area of research with much activity in most universities and research institutions. International conferences are held biennially with an increasing number of participants. With the introduction of sophisticated and continuously im proving instrumentation, biological magnetic resonance is approaching the state of a common physical method in biochemical, biomedical, and bio logical research. The lack of monograpbs on the subject had been con spicuous for a long time. This gap started to close only recently. However, because of the rapid expansion and intensive research, many texts are dated by the time of their appearance. Therefore we have undertaken the editing of a series that is intended to provide the practicing chemist, biochemist, or biologist with the advances and progress in selected contemporary topics. In seeking to make the series as authoritative as possible, we have invited authors who have not only made significant contributions but who are also currently active in their fields. We hope that their expertise as well as their first hand experience as reflected in the chapters of this volume will be of benefit to the reader, inter alia, in planning his own experiments and in critically evaluating the current literature.
Author: Marcus A. Hemminga
Publisher: Springer Science & Business Media
ISBN: 0387250662
Category : Medical
Languages : en
Pages : 346
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Book Description
Starting from a comprehensive quantum mechanical description, this book introduces the optical (IR, Raman, UV/Vis, CD, fluorescence and laser spectroscopy) and magnetic resonance (1D and 2D-NMR, ESR) techniques. The book offers a timely review of the increasing interest in using spin-label ESR as an alternative structural technique for NMR or X-ray diffraction. Future aspects are treated as well, but only as an illustration of the progress of ESR in this field.
Author: Yuri D. Tsvetkov
Publisher:
ISBN: 9783030053734
Category : Electron paramagnetic resonance spectroscopy
Languages : en
Pages : 221
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Book Description
This book covers the basic theory and techniques, as well as various applications of pulsed electron-electron double resonance (PELDOR or DEER). This electron paramagnetic resonance technique is able to measure the distances and the distribution of distances between electron spins in the 1.5-15 nanometer scale; to determine the geometry of spin-labeled molecules; to estimate the number of interacting spins in spin clusters; and to characterize the spatial distribution of paramagnetic centers. As a result, PELDOR is now a popular method in EPR spectroscopy, particularly in the context of biologically important systems and soft matter and is also applied to problems in physical chemistry, biochemistry, polymers, soft matter and materials. Enabling readers to gain an understanding of the fundamentals of the PELDOR methods and an appreciation of the opportunities PELDOR provides, the book helps readers solve their own physical and biochemical problems.
Author: J. Dolinsek
Publisher: Springer
ISBN: 3540326278
Category : Science
Languages : en
Pages : 447
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Book Description
A survey of recent research in the fields of condensed matter physics and chemistry based on novel NMR and ESR techniques. Applications include quantum computing, metal nanoparticles, low dimensional magnets, fullerenes as atomic cages, superconductors, porous media, and laser assisted studies. The book is dedicated to Professor Robert Blinc, on the occasion of his seventieth birthday, in appreciation of his remarkable scientific accomplishments in the NMR of condensed matter.
Author: Gareth R. Eaton
Publisher: World Scientific
ISBN: 9789810232955
Category : Science
Languages : en
Pages : 836
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Book Description
Since its inception 50 years ago, electron paramagnetic resonance (EPR, also called ESR or EMR) has become a major tool in diverse fields ranging from biology and chemistry to solid state physics and materials science. This important book includes personal descriptions of early experiments by pioneers who laid the foundations for the field, perspectives on the state of the art, and glimpses of future opportunities. It presents a broad view of the foundations of EPR and its applications, and will therefore appeal to scientists in many fields. Even the expert will find here history not previously recorded and provocative views of future directions.
Author: Sandra S. Eaton
Publisher: Springer Science & Business Media
ISBN: 9780306485329
Category : Biochemistry
Languages : en
Pages : 504
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Book Description
Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation About the Editors: Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.
Author: Sergei A. Dikanov
Publisher: CRC Press
ISBN: 1040283632
Category : Science
Languages : en
Pages : 430
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Book Description
The first volume devoted entirely to Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy This valuable book provides an introduction and broad survey of topics in ESEEM spectroscopy, including the theory, instrumentation, peculiarities of ESE experiments, and analysis of experimental data with particular emphasis on orientationally disordered systems. Applications of ESEEM spectroscopy to study chemically and biologically important paramagnetic centers in single crystals, amorphous solids, and powders are discussed as well. Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy will benefit specialists in magnetic resonance spectroscopy, physicists, chemists, and biologists who use magnetic resonance in their research.
Author: Bruce C Gilbert
Publisher: Royal Society of Chemistry
ISBN: 1847557562
Category : Science
Languages : en
Pages : 283
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Book Description
Specialist Periodical Reports provide systematic and critical review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject, the series creates a unique service for the active research chemist with regular critical in-depth accounts of progress in particular areas of chemistry. Subject coverage of all volumes is very similar and publication is on an annual or biennial basis. As EPR continues to find new applications in virtually all areas of modern science, including physics, chemistry, biology and materials science, this series caters not only for experts in the field, but also those wishing to gain a general overview of EPR applications in a given area.
