Development of Methods for Directed and Structured Immobilization of Biomolecules PDF Download
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Author: Björn Waterkotte
Publisher: Cuvillier Verlag
ISBN: 3736945329
Category : Science
Languages : en
Pages : 252
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Book Description
Surface immobilized molecules play a crucial role in applied as well as in basic research. They can be found in DNA or protein-array as well as in cell-migration or biochemical interaction experiments. Especially for proteins the immobilization can critically affect molecule accessibility and activity. In this work two essential aspects of protein immobilization were investigated: the identification of suitable surface functionalization by two array approaches and the generation of laterally structured gray scale protein patterns by projection lithography. In proof-of-concept experiments a new strategy to create an array of different, neighboring functionalizations on the same surface was successfully tested. In "mechanical protection" inert plungers were applied to protect distinct surface areas from the surface functionalization reagents and allowed a selective modification of individual regions in serial reactions. In a particle based alternative approach a high spot density surface array was fabricated using functionalized, polyvinyl acetate, melamine and silicon oxide-based particles. The in situ encoded bead-based array (IEBA) allowed the study of immobilization conditions and the effect of protein adhesion. For the reliable and fast analysis of array-images with several thousand individual particles a custom open-source evaluation software was developed. To create protein patterns a custom-built projection lithography device was utilized to generate high definition protein patterns. Patterning conditions were optimized with respect to deposition speed and signal to noise ratio while retaining a biocompatible process flow. The established technology provides protein patterns with great control over the deposited surface density, patterns were transferred within seconds to minutes to functionalized glass slides. To obtain three-dimensional, surface-functionalized surfaces this approach was transferred to thin polymer films. The substrates were demonstrated to be biocompatible with mouse fibroblasts. These surfaces allow a novel level of control addressing both the chemical surrounding and the shape of a cell microenvironment.
Author: Björn Waterkotte
Publisher: Cuvillier Verlag
ISBN: 3736945329
Category : Science
Languages : en
Pages : 252
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Book Description
Surface immobilized molecules play a crucial role in applied as well as in basic research. They can be found in DNA or protein-array as well as in cell-migration or biochemical interaction experiments. Especially for proteins the immobilization can critically affect molecule accessibility and activity. In this work two essential aspects of protein immobilization were investigated: the identification of suitable surface functionalization by two array approaches and the generation of laterally structured gray scale protein patterns by projection lithography. In proof-of-concept experiments a new strategy to create an array of different, neighboring functionalizations on the same surface was successfully tested. In "mechanical protection" inert plungers were applied to protect distinct surface areas from the surface functionalization reagents and allowed a selective modification of individual regions in serial reactions. In a particle based alternative approach a high spot density surface array was fabricated using functionalized, polyvinyl acetate, melamine and silicon oxide-based particles. The in situ encoded bead-based array (IEBA) allowed the study of immobilization conditions and the effect of protein adhesion. For the reliable and fast analysis of array-images with several thousand individual particles a custom open-source evaluation software was developed. To create protein patterns a custom-built projection lithography device was utilized to generate high definition protein patterns. Patterning conditions were optimized with respect to deposition speed and signal to noise ratio while retaining a biocompatible process flow. The established technology provides protein patterns with great control over the deposited surface density, patterns were transferred within seconds to minutes to functionalized glass slides. To obtain three-dimensional, surface-functionalized surfaces this approach was transferred to thin polymer films. The substrates were demonstrated to be biocompatible with mouse fibroblasts. These surfaces allow a novel level of control addressing both the chemical surrounding and the shape of a cell microenvironment.
Author: Anuj Tripathi
Publisher: Springer
ISBN: 9789811580000
Category : Medical
Languages : en
Pages : 666
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Book Description
This book delves into the field of immobilizing biologically active and non-active molecules. It discusses the designing strategy of immobilization and the current state-of-the-art applications for advancing biomedical, agricultural, environmental and industrial practices. It focuses on aspects ranging from fundamental principles to current technological advances at multi-scale levels (macro, micro, and nano) which are suitable for cell, enzyme, and nano-catalyst based applications. Written by experts from across the globe, the contents deal with illustrated examples of molecular and cellular interactions with materials/scaffolds and discussions on factors that can affect the functionality and yield of the process. With its discussions on material science, design of delivery vehicles, separation science, additive manufacturing, agriculture and environmental science, this book will be a useful reference for researchers across multiple disciplines.
Author: Vijay Kumar Thakur
Publisher: John Wiley & Sons
ISBN: 1119044774
Category : Technology & Engineering
Languages : en
Pages : 448
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Book Description
This book addresses surface modification techniques, which are critical for tailoring and broadening the applications of naturally occurring biopolymers. Biopolymers represent a sustainable solution to the need for new materials in the auto, waste removal, biomedical device, building material, defense, and paper industries. Features: First comprehensive summary of biopolymer modification methods to enhance compatibility, flexibility, enhanced physicochemical properties, thermal stability, impact response, and rigidity, among others Address of a green, eco-friendly materials that is increasing in use, underscoring the roles of material scientists in the future of new "green" bioolymer material use Coverage applications in automotive development, hazardous waste removal, biomedical engineering, pulp and paper industries, development of new building materials, and defense-related technologies Facilitation of technology transfer
Author: Van Toi Vo
Publisher: Springer Nature
ISBN: 3031446305
Category : Technology & Engineering
Languages : en
Pages : 1140
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Book Description
This book presents cutting-edge research and developments in the field of biomedical engineering, with a special emphasis on results achieved in Vietnam and neighboring low- and middle-income countries. Covering both fundamental and applied research, and focusing on the theme of “Translational Healthcare Technology from Advanced to Low and Middle Income Countries in the Era of Covid and Digital Transformation”, it reports on the design, fabrication, and application of low-cost and portable medical devices, biosensors, and microfluidic devices, on improved methods for biological data acquisition and analysis, on nanoparticles for biological applications, and on new achievements in biomechanics, tissue engineering, and regeneration. It describes the developments of molecular and cellular biology techniques, neuroengineering techniques, and statistical and computational methods, including artificial intelligence, for biomedical applications. It also discusses strategies to address some relevant issues in biomedical education and entrepreneurship. Gathering the proceedings of the 9th International Conference on The Development of Biomedical Engineering in Vietnam, BME 9, held on December 27-29, 2022, in Ho Chi Minh, Vietnam, the book offers important answers to current challenges in the field and a source of inspiration for scientists, engineers, and researchers with various backgrounds working in different research institutes, companies, and countries.
Author:
Publisher:
ISBN:
Category : Marine pollution
Languages : en
Pages : 718
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Book Description
Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233
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Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author: United States. National Ocean Pollution Program Office
Publisher:
ISBN:
Category : Marine pollution
Languages : en
Pages : 454
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Book Description
Author: Peter Grunwald
Publisher: World Scientific Publishing Company
ISBN: 1783269103
Category : Science
Languages : en
Pages : 1314
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Book Description
In this Completely Revised and Extended Edition with a significantly enhanced content, all Chapters have been updated considering relevant literature and recent developments until 2016 together with application oriented examples with a focus on Industrial Biocatalysis. Newly treated topics comprise among others systems metabolic engineering approaches, metagenome screening, new tools for pathway engineering, and de-novo computational design as actual research areas in biocatalysis. Information about different aspects of RNA technologies, and completely new Chapters on 'Fluorescent Proteins' and 'Biocatalysis and Nanotechnology' are also included.
Author: Hari Singh Nalwa
Publisher: Academic Press
ISBN: 0080533647
Category : Science
Languages : en
Pages : 3593
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Book Description
Nanostructured materials is one of the hottest and fastest growing areas in today's materials science field, along with the related field of solid state physics. Nanostructured materials and their based technologies have opened up exciting new possibilites for future applications in a number of areas including aerospace, automotive, x-ray technology, batteries, sensors, color imaging, printing, computer chips, medical implants, pharmacy, and cosmetics. The ability to change properties on the atomic level promises a revolution in many realms of science and technology. Thus, this book details the high level of activity and significant findings are available for those involved in research and development in the field. It also covers industrial findings and corporate support. This five-volume set summarizes fundamentals of nano-science in a comprehensive way. The contributors enlisted by the editor are at elite institutions worldwide. Key Features * Provides comprehensive coverage of the dominant technology of the 21st century * Written by 127 authors from 16 countries, making this truly international * First and only reference to cover all aspects of nanostructured materials and nanotechnology
Author: Subash C.B. Gopinath
Publisher: Elsevier
ISBN: 9780128139004
Category : Technology & Engineering
Languages : en
Pages : 0
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Book Description
Nanobiosensors for Bio-molecular Targeting presents the latest analytical methods for the detection of different substances in the range of small molecules to whole cells, exploring the advantages and disadvantages of each method. Biosensors combine the component of biological origin and physicochemical detector to show the presence of analytes in a given sample. The use of bionanotechnology has led to a significant advancement in the progression of nanobiosensors and has been effectively used for biomedical diagnosis.
