Detection and Identification of Bacterial Speck of Tomato (Pseudomonas Syringae Pv. Tomato) by Polymerase Chain Reaction PDF Download
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Author: Bilal I. Wreikat
Publisher:
ISBN:
Category :
Languages : en
Pages : 64
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Book Description
Author: Bilal I. Wreikat
Publisher:
ISBN:
Category :
Languages : en
Pages : 64
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Book Description
Author: Diana Carolina Mazo Molina
Publisher:
ISBN:
Category :
Languages : en
Pages : 132
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Book Description
Pseudomonas syringae pv. tomato (Pst) is a persistent pathogen of tomato that causes bacterial speck disease. On tomato, resistance conferred by the gene Pto is effective against race 0 Pst strains which express the effector proteins AvrPto and/or AvrPtoB; however, race 1 strains of Pst, which do not express AvrPto/AvrPtoB but rather a different repertoire of effectors, evade Pto-mediated resistance. Race 1 strains of Pst are becoming increasingly common, and no simply-inherited genetic resistance to such strains is known. It was discovered that a locus in Solanum lycopersicoides, termed Pseudomonas tomato race 1 (Ptr1), confers resistance to race 1 Pst strains by recognizing the type III effector AvrRpt2. In Arabidopsis and apple, strains of Pst and Erwinia amylovora expressing AvrRpt2 degrade the RIN4 protein, thereby activating RPS2 or Mr5-mediated immunity, respectively. Ptr1 also recognized homologs of AvrRpt2 from diverse bacteria including one in Ralstonia pseudosolanacearum and this correlated with the ability of AvrRpt2 to degrade RIN4. Using site-directed mutagenesis of AvrRpt2, we found that, like RPS2, activation of Ptr1 requires AvrRpt2 proteolytic activity. Ptr1 detection of AvrRpt2 activity suggests it likely encodes an NLR protein or possibly a guardee such as RIN4. Ptr1 was identified by cloning of candidate NLR-encoding genes located in the Ptr1 region and testing using Agrobacterium-mediated transient expression in Nicotiana glutinosa identified one gene for the ability to activate the plant immune system in response to AvrRpt2 in the presence of tomato Rin4. Interestingly, while overexpression of Ptr1 in N. glutinosa leaves caused localized cell death, co-expression of Ptr1 with tomato Rin4 prevented this cell death. The protein encoded by Ptr1 has little similarity to RPS2 or Mr5, which suggests that Ptr1 is a third example of convergent evolution in different plant species for recognition of AvrRpt2. In summary, the Ptr1 gene has the potential to become an important component (along with Pto) in controlling bacterial speck disease. Further research focused on studying the mechanism of action between Ptr1 and Rin4 may contribute to a better understanding of the recognition of the type III effector AvrRpt2 in tomato.
Author:
Publisher: GRIN Verlag
ISBN: 3656010935
Category :
Languages : en
Pages : 33
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Author: Douglas Joseph Jardine
Publisher:
ISBN:
Category : Bacterial speck of tomato
Languages : en
Pages : 274
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Author: Susan Getz
Publisher:
ISBN:
Category : Pseudomonas infections
Languages : en
Pages : 168
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Book Description
Author: Nicholas Brian Pyke
Publisher:
ISBN:
Category : Pseudomonas syringae
Languages : en
Pages : 318
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Author: Kathy Roberts Munkvold
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Author: Mindy Michelle James
Publisher:
ISBN:
Category :
Languages : en
Pages : 209
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Book Description
The MLVA fingerprints and MLST profiles from sub-cultured P.s. tomato DC3000 remained consistent throughout the experiment indicating that the selected genetic markers did not change over time. Thus, the specific MLVA and MLST typing systems used in this experiment should be reliable if used in a forensics investigation involving P.s. tomato.
Author: Hikmet Saygili
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 444
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Book Description
Author: P. Narayanasamy
Publisher: Springer Science & Business Media
ISBN: 1402082436
Category : Science
Languages : en
Pages : 262
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Book Description
Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.
