Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques PDF Author: Sean R. Gallagher
Publisher: John Wiley & Sons
ISBN: 047094241X
Category : Science
Languages : en
Pages : 679

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Book Description
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques PDF Author: Sean R. Gallagher
Publisher: John Wiley & Sons
ISBN: 047094241X
Category : Science
Languages : en
Pages : 679

Get Book Here

Book Description
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Short Protocols in Molecular Biology

Short Protocols in Molecular Biology PDF Author: Frederick M. Ausubel
Publisher:
ISBN:
Category : Biología molecular
Languages : en
Pages : 852

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Book Description


Laboratory Protocols in Fungal Biology

Laboratory Protocols in Fungal Biology PDF Author: Vijai Kumar Gupta
Publisher: Springer Science & Business Media
ISBN: 1461423562
Category : Science
Languages : en
Pages : 608

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Book Description
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology PDF Author: Frederick M. Ausubel
Publisher:
ISBN:
Category : Molecular biology
Languages : en
Pages : 1020

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Book Description


Protocols used in Molecular Biology

Protocols used in Molecular Biology PDF Author: Sandeep Singh
Publisher: Bentham Science Publishers
ISBN: 981143929X
Category : Science
Languages : en
Pages : 186

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Book Description
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Gene Therapy of Cancer

Gene Therapy of Cancer PDF Author: Wolfgang Walther
Publisher: Springer Science & Business Media
ISBN: 1592590861
Category : Medical
Languages : en
Pages : 633

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Book Description
Since the discovery of the molecular structure of genes and the unveiling of the molecular basis of numerous human diseases, scientists have been fas- nated with the possibility of treating certain diseases by transducing foreign DNA into the affected cells. Initially, it was proposed that the foreign DNA could either replace defective nonfunctional genes, or code for therapeutic proteins. This concept has evolved into the rapidly growing field of gene therapy. Even though surgery, radiotherapy, and chemotherapy are widely ava- able and routinely used for cancer treatment, these therapies fail to cure approximately 50 percent of cancer patients. Therefore, since it is a disease characterized by aberrant gene expression, cancer has been a target of gene therapy research since the inception of this treatment modality. Numerous cancer gene therapy strategies are currently being investigated, including gene replacement therapy, the regulation of gene expression to modulate immu- logical responses to tumors, the direct killing of tumor cells, and direct int- ference with tumor growth. In this context, gene transfer systems, tumor-specific expression vectors, and novel therapeutic genes have been extensively st- ied. All these strategies aim for the selective destruction of human malignant disease while circumventing the destruction of nonmalignant cells and tissues thereby minimizing toxicity to the patient.

Pyrosequencing Protocols

Pyrosequencing Protocols PDF Author: Sharon Marsh
Publisher: Springer Science & Business Media
ISBN: 1597453773
Category : Science
Languages : en
Pages : 200

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Book Description
This book presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. This volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

The Protein Protocols Handbook

The Protein Protocols Handbook PDF Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 0896039404
Category : Medical
Languages : en
Pages : 1446

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Book Description
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.

Gene Transfer and Expression in Mammalian Cells

Gene Transfer and Expression in Mammalian Cells PDF Author: S.C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Category : Science
Languages : en
Pages : 730

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Book Description
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.

Coronaviruses

Coronaviruses PDF Author: Helena J. Maier
Publisher: Humana
ISBN: 9781071609026
Category : Medical
Languages : en
Pages : 278

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Book Description
This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.