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Author: A. Berthod
Publisher: Elsevier
ISBN: 0080929664
Category : Science
Languages : en
Pages : 310
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Book Description
Countercurrent chromatography (CCC) is a separation technique in which the stationary phase is a liquid. The mobile phase is also a liquid, so biphasic liquid systems with at least two solvents are used. Centrifugal fields are used to hold the liquid stationary phase while pushing the liquid mobile phase through it. This comprehensive reference covers recent advancements in the two types of CCC machines: the high speed CCCs without rotary seals and with coiled spools and centrifugal partition chromatographs (CPC) with rotary seals and interconnected channels. Written by leading international experts in the CCC field, the book focuses on the liquid nature of the stationary phase: giving newcomers the basis to do CCC efficiently and rapidly; explaining the art of obtaining a biphasic liquid system; describing the flow patterns in both CPC and high speed CCC machines; showing possible other uses of a liquid stationary phase; presenting a wealth of applications in the separation of organic, pharmaceutical and inorganic mixtures; and demonstrating that even supercritical fluids can be used in CCC.
Author: A. Berthod
Publisher: Elsevier
ISBN: 0080929664
Category : Science
Languages : en
Pages : 310
Get Book
Book Description
Countercurrent chromatography (CCC) is a separation technique in which the stationary phase is a liquid. The mobile phase is also a liquid, so biphasic liquid systems with at least two solvents are used. Centrifugal fields are used to hold the liquid stationary phase while pushing the liquid mobile phase through it. This comprehensive reference covers recent advancements in the two types of CCC machines: the high speed CCCs without rotary seals and with coiled spools and centrifugal partition chromatographs (CPC) with rotary seals and interconnected channels. Written by leading international experts in the CCC field, the book focuses on the liquid nature of the stationary phase: giving newcomers the basis to do CCC efficiently and rapidly; explaining the art of obtaining a biphasic liquid system; describing the flow patterns in both CPC and high speed CCC machines; showing possible other uses of a liquid stationary phase; presenting a wealth of applications in the separation of organic, pharmaceutical and inorganic mixtures; and demonstrating that even supercritical fluids can be used in CCC.
Author: Jean-Michel Menet
Publisher: CRC Press
ISBN: 1482273802
Category : Science
Languages : en
Pages : 298
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Book Description
Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, a versatile technique for purificati
Author: Colin F. Poole
Publisher: Academic Press
ISBN: 9780122267703
Category : Science
Languages : en
Pages : 0
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Book Description
The Encyclopedia of Separation Science is the most comprehensive resource available on the theory, techniques, and applications of separation science. The work presents information on three levels. The first volume contains Level 1, which provides a broad overview of the theory of the 12 main categories of separation techniques. Volumes 2-4 (Level 2) expand coverage with detailed theoretical and technical descriptions of particular techniques. The remaining Volumes 5-9 (Level 3) cover applications of these techniques from the micro to the macro, and also from the analytical laboratory bench to large-scale industrial processes. Volume 10 consists mainly of the index. Initial access to the online version offering extensive hypertext linking and advanced search tools is available with purchase. Ongoing access is maintained for a minimum annual fee. The Encyclopedia of Separation Science is the first truly comprehensive work covering the whole of separation theory, methods, and techniques. This encyclopedia will be invaluable to researchers and professionals across a wide range of areas in academia and industry. Enclyclopedia of Separation Science is available online via ScienceDirect offering enhanced features such as extensive cross-referencing and dynamic linking. For more information please (http://www.info.sciencedirect.com/reference_works/works_available/separation/index.shtml click here.)
Author: Jean-Michel Menet
Publisher: CRC Press
ISBN: 9780824799922
Category : Science
Languages : en
Pages : 302
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Book Description
Covering definitions, concepts, and applications, Countercurrent Chromatography recounts the developments in two types of liquid-liquid chromatography termed countercurrent-high-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC)-as well as the HSCCC-derived cross-axis CCC, a versatile technique for purification in biotechnology applications. The text investigates mechanisms for mixing liquid phases, particularly hydrostatic techniques for CPC and hydrodynamic for coil planet centrifuges. It also explores the use of countercurrent chromatography in inorganic analysis, chiral separation, and the separation of natural products.
Author: Walter D. Conway
Publisher: Wiley-VCH
ISBN:
Category : Science
Languages : en
Pages : 504
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Book Description
Author: Walter D. Conway
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 256
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Book Description
Presents theoretical and practical aspects of the most recent advances in countercurrent chromatography, including pH-zone-refining countercurrent chromatography. Examines the use of countercurrent chromatography for the measurement of octanol/water partition coefficients. Describes separation of alkaloids and other pharmacologically active substances from plants. Discusses optimization of solvent systems and reviews the purification of peptides, lipoproteins, and commercial dyes. Includes information on multilayer coil and cross axis countercurrent chromatography and presents theoretical models for comparison and optimization.
Author: Yoichiro Ito
Publisher: Wiley-Interscience
ISBN:
Category : Science
Languages : en
Pages : 488
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Book Description
High-Speed Countercurrent Chromatography is the first book to provide a comprehensive and up-to-date treatment of this technique. It covers all the latest developments in equipment, theory, and applications, as well as many topics not previously published anywhere, such as the purification of recombinant proteins directly from a crude E. coli lysate, the development of instruments that produce highly concentrated pure fractions, and successful CCC/MS interfacing.
Author: Alain Berthod
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: G. Ganetsos
Publisher: CRC Press
ISBN: 9780824787387
Category : Science
Languages : en
Pages : 812
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Book Description
Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.
Author: K. Hostettmann
Publisher: Springer Science & Business Media
ISBN: 3662036312
Category : Science
Languages : en
Pages : 259
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Book Description
Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.
