Author: Jonathan Robert Beck
Publisher:
ISBN:
Category : Chemical kinetics
Languages : en
Pages : 218
Book Description
Analysis of large molecules from proteins to peptides and even higher mass species such as DNA is an important task, and in recent years, has been a specialty of mass spectrometry. Gaining structural information is possible using multi stage mass spectrometric techniques (MS) n . The technique of surface induced dissociation (SID) of singly protonated peptides ionized by matrix assisted laser desorption/ionization and analyzed by a tandem time-of-flight mass spectrometer with a collision surface located between the two time-of-flight mass spectrometers is described here. The identity of positively charged product ions from SID is dependent on the velocity of the parent ions colliding at near normal angles to the SID surface. This dependence on collision velocity is evident for the entire mass range of peptides studied (280-12,384 amu). The observed trend suggests that SID has the potential to fragment very large ions, yielding product ions of certain identities, as long as, the necessary collision energy/velocity can be achieved. SID of the protonated peptides produces product ions that fall into categories of the following types of product ions: intact molecular ions, backbone fragments, single amino acid immonium ions (H 2 N = CH-R) +, immonium ion fragments, and finally, sputtered surface material. The range of collision energies over which these products occurs changes based upon the mass of the ion, but is constant for collision velocity. The ranges of collision velocities for the different types of product ions are as follows: intact molecular ions and backbone fragments (3500 m/s), single amino acid immonium ions (2600-5000 m/s), fragments of immonium ions (5000-13,000 m/s), and sputtered ions from the surface (13,200 m/s).
Collision Velocity Dependence of Products Formed Via Surface Induced Dissociation
Author: Jonathan Robert Beck
Publisher:
ISBN:
Category : Chemical kinetics
Languages : en
Pages : 218
Book Description
Analysis of large molecules from proteins to peptides and even higher mass species such as DNA is an important task, and in recent years, has been a specialty of mass spectrometry. Gaining structural information is possible using multi stage mass spectrometric techniques (MS) n . The technique of surface induced dissociation (SID) of singly protonated peptides ionized by matrix assisted laser desorption/ionization and analyzed by a tandem time-of-flight mass spectrometer with a collision surface located between the two time-of-flight mass spectrometers is described here. The identity of positively charged product ions from SID is dependent on the velocity of the parent ions colliding at near normal angles to the SID surface. This dependence on collision velocity is evident for the entire mass range of peptides studied (280-12,384 amu). The observed trend suggests that SID has the potential to fragment very large ions, yielding product ions of certain identities, as long as, the necessary collision energy/velocity can be achieved. SID of the protonated peptides produces product ions that fall into categories of the following types of product ions: intact molecular ions, backbone fragments, single amino acid immonium ions (H 2 N = CH-R) +, immonium ion fragments, and finally, sputtered surface material. The range of collision energies over which these products occurs changes based upon the mass of the ion, but is constant for collision velocity. The ranges of collision velocities for the different types of product ions are as follows: intact molecular ions and backbone fragments (3500 m/s), single amino acid immonium ions (2600-5000 m/s), fragments of immonium ions (5000-13,000 m/s), and sputtered ions from the surface (13,200 m/s).
Publisher:
ISBN:
Category : Chemical kinetics
Languages : en
Pages : 218
Book Description
Analysis of large molecules from proteins to peptides and even higher mass species such as DNA is an important task, and in recent years, has been a specialty of mass spectrometry. Gaining structural information is possible using multi stage mass spectrometric techniques (MS) n . The technique of surface induced dissociation (SID) of singly protonated peptides ionized by matrix assisted laser desorption/ionization and analyzed by a tandem time-of-flight mass spectrometer with a collision surface located between the two time-of-flight mass spectrometers is described here. The identity of positively charged product ions from SID is dependent on the velocity of the parent ions colliding at near normal angles to the SID surface. This dependence on collision velocity is evident for the entire mass range of peptides studied (280-12,384 amu). The observed trend suggests that SID has the potential to fragment very large ions, yielding product ions of certain identities, as long as, the necessary collision energy/velocity can be achieved. SID of the protonated peptides produces product ions that fall into categories of the following types of product ions: intact molecular ions, backbone fragments, single amino acid immonium ions (H 2 N = CH-R) +, immonium ion fragments, and finally, sputtered surface material. The range of collision energies over which these products occurs changes based upon the mass of the ion, but is constant for collision velocity. The ranges of collision velocities for the different types of product ions are as follows: intact molecular ions and backbone fragments (3500 m/s), single amino acid immonium ions (2600-5000 m/s), fragments of immonium ions (5000-13,000 m/s), and sputtered ions from the surface (13,200 m/s).
Interaction Mechanism Leading to the Formation of Ion-surface Association Products During Surface-induced Dissociation Tandem Mass Spectrometry
Author: Lynn Marie Phelan
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
Book Description
Molecular Reaction Dynamics
Author: Raphael D. Levine
Publisher: Cambridge University Press
ISBN: 9781139442879
Category : Technology & Engineering
Languages : en
Pages : 574
Book Description
Molecular reaction dynamics is the study of chemical and physical transformations of matter at the molecular level. The understanding of how chemical reactions occur and how to control them is fundamental to chemists and interdisciplinary areas such as materials and nanoscience, rational drug design, environmental and astrochemistry. This book provides a thorough foundation to this area. The first half is introductory, detailing experimental techniques for initiating and probing reaction dynamics and the essential insights that have been gained. The second part explores key areas including photoselective chemistry, stereochemistry, chemical reactions in real time and chemical reaction dynamics in solutions and interfaces. Typical of the new challenges are molecular machines, enzyme action and molecular control. With problem sets included, this book is suitable for advanced undergraduate and graduate students, as well as being supplementary to chemical kinetics, physical chemistry, biophysics and materials science courses, and as a primer for practising scientists.
Publisher: Cambridge University Press
ISBN: 9781139442879
Category : Technology & Engineering
Languages : en
Pages : 574
Book Description
Molecular reaction dynamics is the study of chemical and physical transformations of matter at the molecular level. The understanding of how chemical reactions occur and how to control them is fundamental to chemists and interdisciplinary areas such as materials and nanoscience, rational drug design, environmental and astrochemistry. This book provides a thorough foundation to this area. The first half is introductory, detailing experimental techniques for initiating and probing reaction dynamics and the essential insights that have been gained. The second part explores key areas including photoselective chemistry, stereochemistry, chemical reactions in real time and chemical reaction dynamics in solutions and interfaces. Typical of the new challenges are molecular machines, enzyme action and molecular control. With problem sets included, this book is suitable for advanced undergraduate and graduate students, as well as being supplementary to chemical kinetics, physical chemistry, biophysics and materials science courses, and as a primer for practising scientists.
Dissertation Abstracts International
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1006
Book Description
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 1006
Book Description
Mass Spectrometry/mass Spectrometry
Author: Kenneth L. Busch
Publisher:
ISBN:
Category : Mass spectrometry
Languages : en
Pages : 360
Book Description
Publisher:
ISBN:
Category : Mass spectrometry
Languages : en
Pages : 360
Book Description
Nuclear Science Abstracts
Author:
Publisher:
ISBN:
Category : Nuclear energy
Languages : en
Pages : 1288
Book Description
Publisher:
ISBN:
Category : Nuclear energy
Languages : en
Pages : 1288
Book Description
Nuclear Power Reactor Instrumentation Systems Handbook
Author: Joseph M. Harrer
Publisher:
ISBN: 9780870790058
Category : Technology & Engineering
Languages : en
Pages : 1052
Book Description
Publisher:
ISBN: 9780870790058
Category : Technology & Engineering
Languages : en
Pages : 1052
Book Description
Spectroscopy and Modeling of Biomolecular Building Blocks
Author: Jean-Pierre Schermann
Publisher: Elsevier
ISBN: 0080558224
Category : Science
Languages : en
Pages : 499
Book Description
Spectroscopy and Modeling of Biomolecular Building Blocks presents an overview of recent advances in the intertwining of the following research fields: photon and electron spectroscopy, quantum chemistry, modelling and mass-spectrometry. The coupling of these disciplines offers a new point of view to the understanding of isolated elementary building blocks of biomolecules and their assemblies. It allows the unambiguous separation between intrinsic properties of biomolecular systems and those induced by the presence of their environment. The first chapters provide background in modelling (I), frequency-resolved spectroscopy using microwave, infrared and UV photons, time-resolved spectroscopy in the femtosecond domain and energy-resolved electron spectroscopy (II) and production of gas-phase neutral and ionic biomolecular species, mass-spectrometry, ion mobility and BIRD techniques (III). Chapter IV is devoted to case studies of gas-phase experimental investigations coupled to quantum or classical calculations. The topics are structural studies of nucleobases and oligonucleotides, peptides and proteins, sugars; neuromolecules; non-covalent complexes; chiral systems, interactions of low-energy electrons with biomolecules in the radiation chemistry context and very large gas-phase biomolecular systems. The fifth chapter concerns the link between gas-phase and liquid-phase. Different treatments of solvation are illustrated through examples pointing out the influence of progressive addition of water molecules upon properties of nucleobases, peptides, sugars and neuromolecules. - Offer a new perspective to the understanding of isolated elementary building blocks of bio molecules - Includes case studies of experimental investigations coupled to quantum or classical calculations
Publisher: Elsevier
ISBN: 0080558224
Category : Science
Languages : en
Pages : 499
Book Description
Spectroscopy and Modeling of Biomolecular Building Blocks presents an overview of recent advances in the intertwining of the following research fields: photon and electron spectroscopy, quantum chemistry, modelling and mass-spectrometry. The coupling of these disciplines offers a new point of view to the understanding of isolated elementary building blocks of biomolecules and their assemblies. It allows the unambiguous separation between intrinsic properties of biomolecular systems and those induced by the presence of their environment. The first chapters provide background in modelling (I), frequency-resolved spectroscopy using microwave, infrared and UV photons, time-resolved spectroscopy in the femtosecond domain and energy-resolved electron spectroscopy (II) and production of gas-phase neutral and ionic biomolecular species, mass-spectrometry, ion mobility and BIRD techniques (III). Chapter IV is devoted to case studies of gas-phase experimental investigations coupled to quantum or classical calculations. The topics are structural studies of nucleobases and oligonucleotides, peptides and proteins, sugars; neuromolecules; non-covalent complexes; chiral systems, interactions of low-energy electrons with biomolecules in the radiation chemistry context and very large gas-phase biomolecular systems. The fifth chapter concerns the link between gas-phase and liquid-phase. Different treatments of solvation are illustrated through examples pointing out the influence of progressive addition of water molecules upon properties of nucleobases, peptides, sugars and neuromolecules. - Offer a new perspective to the understanding of isolated elementary building blocks of bio molecules - Includes case studies of experimental investigations coupled to quantum or classical calculations
Energy Research Abstracts
Author:
Publisher:
ISBN:
Category : Power resources
Languages : en
Pages : 970
Book Description
Publisher:
ISBN:
Category : Power resources
Languages : en
Pages : 970
Book Description
Scientific and Technical Aerospace Reports
Author:
Publisher:
ISBN:
Category : Aeronautics
Languages : en
Pages : 380
Book Description
Lists citations with abstracts for aerospace related reports obtained from world wide sources and announces documents that have recently been entered into the NASA Scientific and Technical Information Database.
Publisher:
ISBN:
Category : Aeronautics
Languages : en
Pages : 380
Book Description
Lists citations with abstracts for aerospace related reports obtained from world wide sources and announces documents that have recently been entered into the NASA Scientific and Technical Information Database.