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Author: Saeid Kadkhodaei
Publisher: Springer
ISBN: 3319903918
Category : Science
Languages : en
Pages : 121
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Book Description
This book is a practical review which focuses on computational analysis and on in silico approaches towards the systematic discovery of various key functional gene expression elements in microalgae as a model. So far, in this regard very little information is available. Efficient stepwise procedures for analysing the matrix attachment regions (MARs) are outlined, as well as for translation initiation sites (TIS), signal peptide (SP) sequences, gene optimization and transformation systems. These outlines can be efficiently deployed as practical models for the systematic discovery of key expression elements and for the optimization of cis/transgenes in other micro/organisms. The first chapter is an introduction on the key gene expression elements analysed in this book, including scaffold/matrix attachment regions, translation initiation sites, signal peptides as well as gene optimization. Chapter 2 focuses on systematic strategies and computational approaches toward in silico analysis of each factor. The analyses outcomes is assessed individually in chapter 3 followed by developing the specific conceptual models for each element in Chapter 4. The concluding remarks are discussed in Chapter 5. This work is of interest to computational and experimental biologists interested in transcriptional regulation analysis as well as to researchers and scientists who wish to consider the use of bioinformatics and computational biology in design, analysis, or regulatory reviews of key gene expression elements for the production of recombinant proteins experiments.
Author: Saeid Kadkhodaei
Publisher: Springer
ISBN: 3319903918
Category : Science
Languages : en
Pages : 121
Get Book Here
Book Description
This book is a practical review which focuses on computational analysis and on in silico approaches towards the systematic discovery of various key functional gene expression elements in microalgae as a model. So far, in this regard very little information is available. Efficient stepwise procedures for analysing the matrix attachment regions (MARs) are outlined, as well as for translation initiation sites (TIS), signal peptide (SP) sequences, gene optimization and transformation systems. These outlines can be efficiently deployed as practical models for the systematic discovery of key expression elements and for the optimization of cis/transgenes in other micro/organisms. The first chapter is an introduction on the key gene expression elements analysed in this book, including scaffold/matrix attachment regions, translation initiation sites, signal peptides as well as gene optimization. Chapter 2 focuses on systematic strategies and computational approaches toward in silico analysis of each factor. The analyses outcomes is assessed individually in chapter 3 followed by developing the specific conceptual models for each element in Chapter 4. The concluding remarks are discussed in Chapter 5. This work is of interest to computational and experimental biologists interested in transcriptional regulation analysis as well as to researchers and scientists who wish to consider the use of bioinformatics and computational biology in design, analysis, or regulatory reviews of key gene expression elements for the production of recombinant proteins experiments.
Author: Michael G. Kaplitt
Publisher: Gulf Professional Publishing
ISBN: 9780123976321
Category : Medical
Languages : en
Pages : 380
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Book Description
Few areas of biomedical research provide greater opportunities to capitalize upon the revolution in genomics and molecular biology than gene therapy. This is particularly true for the brain and nervous system, where gene transfer has become a key technology for basic research and has recently been translated to human therapy in several landmark clinical trials. Gene Therapy in the Brain: From Bench to Bedside represents the definitive volume on this subject. Edited by two pioneers of neurological gene therapy, this volume contains contributions by leaders who helped to create the field as well as those who are expanding the promise of gene therapy for the future of basic and clinical neuroscience. Drawing upon this extensive collective experience, this book provides clear and informative reviews on a variety of subjects which would be of interest to anyone who is currently using or contemplating exploring gene therapy for neurobiological applications. Basic gene transfer technologies are discussed, with particular emphases upon novel vehicles, immunological issues and the role of gene therapy in stem cells. Numerous research applications are reviewed, particularly in complex fields such as behavioral neurobiology. Several preclinical areas are also covered which are likely to translate into clinical studies in the near future, including epilepsy, pain and amyotrophic lateral sclerosis. Among the most exciting advances in recent years has been the use of neurological gene therapy in human clinical trials, including Parkinson's disease, Canavan disease and Batten disease. Finally, readers will find "insider" information on technological and regulatory issues which can often limit effective translation of even the most promising idea into clinical use. This work provides up-to-date information and key insights into those gene therapy issues which are important to both scientists and clinicians focusing upon the brain and central nervous system.
Author: Didier Trono
Publisher: Springer Science & Business Media
ISBN: 9783540421900
Category : Medical
Languages : en
Pages : 276
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Book Description
For the first time a compilation of chapters that depict the biological bases underlying the development of lentiviral vectors, the techniques involved in the manufacture of this new gene delivery tool, and its most promising applications.
Author: Bhupendra Koul
Publisher: Springer Nature
ISBN: 9811921199
Category : Technology & Engineering
Languages : en
Pages : 442
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Book Description
This book presents up-to-date information on various vector-less/direct (physical, chemical) and vector-mediated/indirect (Agrobacterium-mediated) plant transformation techniques. It summarizes various strategies that facilitate a gene from lower organism to be expressed in higher plants and also in silico designing of synthetic gene for higher expression. It also highlights the importance of strong promoters to drive the expression of transgene(s). This book encompasses the advantages and drawbacks of cisgenesis and transgenesis, their implications towards sustainable crop improvement, and their future prospects. The importance, limitations, challenges, recent developments, and future prospects of molecular pharming is also discussed. The book concludes with a chapter that summarizes the major contribution of GM-crops towards global food security and economy, advances in genome editing for crop improvement, challenges and risk associated with the release of GM-crops, and the future of GM technology. This book is meant for students and researchers in the field of life sciences, food science, and agriculture.
Author: J.-D. Rochaix
Publisher: Springer Science & Business Media
ISBN: 0792351746
Category : Science
Languages : en
Pages : 736
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Book Description
Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR
Author: Ingo Potrykus
Publisher: Springer Science & Business Media
ISBN: 3642792472
Category : Science
Languages : en
Pages : 370
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Book Description
Author: Sant Saran Bhojwani
Publisher: Springer Science & Business Media
ISBN: 9401712034
Category : Science
Languages : en
Pages : 534
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Book Description
The revolutionary progress made in this fascinating field of sexual reproduction inspired this generously illustrated volume. It includes 21 chapters written by experts, covering all aspects of the embryology of angiosperms, ranging from development, isolation, and structure of gametes to endosperm and seed development.
Author: Dongsheng Duan
Publisher: Springer
ISBN: 3030030954
Category : Medical
Languages : en
Pages : 804
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Book Description
About 7 million people worldwide are suffering from various inherited neuromuscular diseases. Gene therapy brings the hope of treating these diseases at their genetic roots. Muscle Gene Therapy is the only book dedicated to this topic. The first edition was published in 2010 when the field was just about to enter its prime time. The progress made since then has been unprecedented. The number of diseases that have been targeted by gene therapy has increased tremendously. The gene therapy toolbox is expanded greatly with many creative novel strategies (such as genome editing and therapy with disease-modifying genes). Most importantly, clinical benefits have begun to emerge in human patients. To reflect rapid advances in the field, we have compiled the second edition of Muscle Gene Therapy with contributions from experts that have conducted gene therapy studies either in animal models and/or in human patients. The new edition offers a much needed, up-to-date overview and perspective on the foundation and current status of neuromuscular disease gene therapy. It provides a framework to the development and regulatory approval of muscle gene therapy drugs in the upcoming years. This book is a must-have for anyone who is interested in neuromuscular disease gene therapy including those in the research arena (established investigators and trainees in the fields of clinical practice, veterinary medicine and basic biomedical sciences), funding and regulatory agencies, and patient community.
Author: Rishabha Malviya
Publisher: CRC Press
ISBN: 1040052118
Category : Medical
Languages : en
Pages : 235
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Book Description
Neurological illnesses pose one of the biggest hazards to the healthcare system today. This new book brings together the latest methods of gene therapy that can be used to treat both inherited and sporadic neurodegenerative diseases. It presents the most significant advances in gene transfer methods as well as the most recent understandings of the mechanisms behind specific neurodegenerative illnesses, placing these into the context of gene therapy approaches for the central nervous system. The book introduces the basics of neurodegenerative conditions and the physiological basis for their occurrence in humans. It also discusses the various proteins useful in neuro diseases and their viral applications. The book explores how stem cells can be used to learn about the future of gene therapy along with the significance of the sequences that silence genes. It discusses the functions of astrocytes in different brain regions, as well as in-vivo research in gene therapy for neurodegenerative disorders.
Author: Nicola Brunetti-Pierri
Publisher: Springer
ISBN: 3319534572
Category : Medical
Languages : en
Pages : 222
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Book Description
In this book, leading international experts analyze state-of-the-art advances in gene transfer vectors for applications in inherited disorders and also examine the toxicity profiles of these methods. The authors discuss the strengths and weaknesses of available vectors in the clinical setting, and specifically focus on the challenges and possible solutions that researchers are testing in order to improve the safety of gene therapy for genetic diseases. This comprehensive and authoritative overview of vector development is a necessary text for researchers, toxicologists, pharmacologists, molecular biologists, physicians, and students in these fields.
