Characterization of Two Distinct Transcriptional Regulators Encoded by the GCR1 Gene in the Yeast Saccharomyces Cerevisiae PDF Download
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Author: Young-Kwon Hong
Publisher:
ISBN:
Category :
Languages : en
Pages : 354
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Book Description
Author: Young-Kwon Hong
Publisher:
ISBN:
Category :
Languages : en
Pages : 354
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Book Description
Author: Nagarajan Lakshmanan
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 0
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Book Description
YAL044, a gene on the left arm of Saccharomyces cerevisiae chromosome one, is shown to code for the H-protein subunit of the multienzyme glycine cleavage system. The gene designation has therefore been changed from YAL044 to GCV3 to reflect its role in the glycine cleavage system. GCV3 encodes a 177 amino acid residue protein with a putative mitochondrial targeting sequence at its amino terminus. Targeted gene replacement shows that GCV3 is not essential for growth on minimal media. It is, however, essential for growth when glycine serves as the sole nitrogen source. Studies of GCV3 expression revealed that it is highly regulated. Supplement with glycine, the glycine cleavage system's substrate, induced expression at least 30-fold. In contrast, addition of the C1-metabolic end products repressed expression about 10-fold. The regulation of glycine cleavage system activity reflects the availability of glycine and the cellular demand for its metabolic products. In addition the glycine cleavage system has been shown to be important for the growth and viability of organisms ranging from microorganisms such as E. coli and S. cerevisiae to humans. Although, this system is important and its activity highly regulated little was known about the transcriptional regulatory mechanisms that control its activity. To address this I have examined the transcriptional regulation of the S. cerevisiae GCV3 gene. The results presented here show that at least six different transcriptional activators control GCV3 expression. These include: an as yet unidentified activator that is partially responsible for its induction by glycine; Gcn4p the transcriptional activator that mediates general amino acid control; Gln3p which is involved in the activation of nitrogen regulated genes; Gcr1p, a transcription factor important for the expression of glycolytic genes; Bas1p/Bas2p which cooperatively mediates the glycine-dependent expression; and an as yet unidentified factor that represses expression regardless of the growth condition. Additional evidence suggests that Rap1p, Nil1p, Acr1p, Ure2p, and Da180p also regulate GCV3 regulation.
Author: Warren Alden Kibbe
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 536
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Book Description
The yeast Saccharomyces cerevisiae contains a family of hsp70 related genes. One member of this family, SSA1, encodes a 70kD heat-shock protein which in addition to its heat inducible expression has a significant basal level of expression. The first 500 bp upstream of the SSA1 start point of transcription was examined by DNAse I protection analysis. The results reveal the presence of at least 14 factor binding sites throughout the upstream promoter region. The function of these binding sites has been examined using a series of 5' promoter deletions fused to the recorder gene lacZ in a centromere-containing yeast shuttle vector. The following sites have been identified in the promoter and their activity in yeast determined individually with a centromere-based recorder plasmid containing a truncated CYC1 /lacZ fusion: a heat-shock element or HSE which is sufficient to convey heat-shock response on the recorder plasmid; a homology to the SV40 'core' sequence which can repress the GCN4 recognition element (GCRE) and the yAP1 recognition element (ARE), and has been designated a upstream repression element or URE; a 'G'-rich region named G-box which can also convey heatshock response on the recorder plasmid; and a purine-pyrimidine alternating sequence name GT-box which is an activator of transcription. A series of fusion constructs were made to identify a putative silencer-like element upstream of SSA1. This element is position dependent and has been localized to a region containing both an ABF1 binding site and a RAP1 binding site. Five site-specific DNA-binding factors are identified and their purification is presented: the heat-shock transcription factor or HSTF, which recognizes the HSE; the G-box binding factor or GBF; the URE recognition factor or URF; the GT-box binding factor; and the GC-box binding factor or yeast Sp1.
Author: Christopher T. Harbison
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
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Book Description
Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring considerable time and effort. Microarray technology now enables investigation of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a population of cells, as well as to locate the regions within the genome that are bound by transcriptional regulators. Such studies not only allow for the functional annotation of both genes and regulators, but can also provide clues about the identity of the regulatory regions within DNA, the structure of global regulatory networks and the regulation of DNA-binding proteins. These and other insights are presented here based on our genome-wide studies of transcriptional regulation in the yeast Saccharomyces cerevisiae.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1200
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Author: J. Richard Dickinson
Publisher: CRC Press
ISBN: 0203503864
Category : Science
Languages : en
Pages : 476
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Book Description
Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and r
Author: Dilip K Arora
Publisher: Elsevier
ISBN: 0080461328
Category : Science
Languages : en
Pages : 445
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Book Description
This latest volume addresses the contemporary issues related to recombination in filamentous fungi, EST data mining, fungal intervening sequences, gene silencing, DNA damage response in filamentous fungi, cfp genes of Neurospora, developmental gene sequences, site-specific recombination, heterologous gene expression, hybridization and microarray technology to enumerate biomass. This volume also analyse the current knowledge in the area of hydrophobins and genetic regulation of carotenoid biosynthesis. Over fifty world renowned scientist from both industry and academics provided in-depth information in the field of fungal genes and genomics.
Author: Christina Smolke
Publisher: John Wiley & Sons
ISBN: 3527688099
Category : Science
Languages : en
Pages : 532
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Book Description
A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.
Author: Catherine Elizabeth Willett
Publisher:
ISBN:
Category :
Languages : en
Pages : 366
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Book Description
Author: Friedrich K. Zimmermann
Publisher: CRC Press
ISBN: 9781566764667
Category : Technology & Engineering
Languages : en
Pages : 590
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Book Description
Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.
