Characterization of Factors Involved in Cleavage and Polyadenylation of Mammalian Messenger RNA Precursors PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Characterization of Factors Involved in Cleavage and Polyadenylation of Mammalian Messenger RNA Precursors PDF full book. Access full book title Characterization of Factors Involved in Cleavage and Polyadenylation of Mammalian Messenger RNA Precursors by Sabine Dettwiler. Download full books in PDF and EPUB format.
Author: Sabine Dettwiler
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
Get Book Here
Book Description
Author: Sabine Dettwiler
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
Get Book Here
Book Description
Author: Ursula Rüegsegger
Publisher:
ISBN:
Category :
Languages : en
Pages : 268
Get Book Here
Book Description
Author: Henk de Vries
Publisher:
ISBN:
Category :
Languages : en
Pages : 194
Get Book Here
Book Description
Author: Silke Andrea Bienroth
Publisher:
ISBN:
Category :
Languages : en
Pages : 123
Get Book Here
Book Description
Author: Silke Bienroth
Publisher:
ISBN:
Category :
Languages : fr
Pages : 246
Get Book Here
Book Description
Author: Sabine Krause
Publisher:
ISBN:
Category :
Languages : en
Pages : 216
Get Book Here
Book Description
Author: Hongwei Zhao
Publisher:
ISBN:
Category : Messenger RNA.
Languages : en
Pages : 126
Get Book Here
Book Description
Cleavage and polyadenylation of precursor messenger RNA (pre-mRNA) is an essential processing event during mRNA maturation. Most of the understanding of the protein factors required for this process comes from yeast and mammals in which successful in vitro cleavage and polyadenylation systems have been extensively employed. In contrast, limited information about plant polyadenylation factors and their functions has been largely from bioinformatics and genetics studies more recently, due to the lack of a plant in vitro system. Using tandem affinity purification (TAP) methods, plant cleavage and polyadenylation specificity factor (CPSF) was isolated and identified from Arabidopsis thaliana. By compiling the in vivo interaction data from TAP purification, as well as available yeast two-hybrid assay data from literature, a plant CPSF model is proposed. According to this model, the Arabidopsis CPSF possesses AtCPSF30, AtCPSF73-I, AtCPSF73-II, AtCPSF100, AtCPSF160, AtFY, and AtFIPS5. Among them, AtCPSF100 serves as a core on which other factors are associated, except for AtFIPS5. AtFIPS5 associates to the CPSF complex indirectly by interacting with AtCPSF30. Protein factors from other complexes, such as AtSYM5, AtCLPS3 and AtPCFS4, may associate with CPSF through AtCPSF30 directly or indirectly. These results show that plant CPSF shares many similarities with its yeast and mammalian counterparts, despite some obvious distinct features. To reconstitute an in vitro assay system for plant mRNA 3'-end processing, protein extracts from Arabidopsis suspension cell cultures over-expressing some polyadenylation-related protein factors, such as AtCPSF30, AtCPSF73-I, AtCPSF73-II, and AtCLPS3, were successfully used in regenerating the cleavage reaction. The characteristics of the plant in vitro cleavage system were further studied by using one of these over-expressed factors, AtCPSF73-I. This in vitro cleavage system has the expected features: cleaves the substrate RNA at the same locations as when processed in vivo; the cleavage activity is dependent on the polyadenylation signals; and the cleavage leaves a 3'-hydroxyl group at the 3'-end for polyadenylation. Analysis of the system also showed the potential involvement of both endonuclease and 3'-5'exonuclease activities. An endo-exonuclease dualism model was proposed for pre-mRNA cleavage in plants. This pioneering system will serve as a platform to further explore plant mRNA 3'-end formation mechanisms and other RNA processing events.
Author: Daniel R. Schoenberg
Publisher: Humana
ISBN: 9781588292254
Category : Science
Languages : en
Pages : 270
Get Book Here
Book Description
Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this series (RNA–Protein Interaction Protocols, Susan Haynes, ed.) and specialized methods journals. In selecting topics for mRNA Processing and Metabolism: Methods and Protocols, I sought input on new and novel techniques and approaches that build on this foundation using technological advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference. I have tried not to bias this book toward any single model organism, and approaches described in the various chapters use yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells.
Author:
Publisher:
ISBN: 9781071608340
Category : Gene expression
Languages : en
Pages :
Get Book Here
Book Description
Author: Eric Sau-Chum Ho
Publisher:
ISBN:
Category : Bioinformatics
Languages : en
Pages : 200
Get Book Here
Book Description
Most eukaryotic protein coding precursor messenger RNAs (pre-mRNAs) undergo polyadenylation after transcription. Polyadenylation is a two-step enzymatic reaction, in which the emerging pre-mRNA is cleaved from the transcription complex, and then followed by the polymerization of adenosine nucleotides starting from the cleaved 3' end to form the poly(A) tail. Biologically, poly(A) tail increases mRNA stability, protein translatability, and mRNA nuclear export. Surprisingly, large numbers of protein factors were found to be involved in this apparently simple cleavage and polymerization steps, suggesting that polyadenylation is under complex regulation. Hence in this study, I am interested to investigate the regulatory elements of eukaryotic polyadenylation. The proposed close species comparison approach revealed an asymmetric selection pressure around the polyadenylation cleavage site (PAS). The region from the PAS to approximately 200 nucleotides (nts) upstream was found to be under a much higher conservation than the downstream region and other part of the 3ÚTR. Furthermore, over 2,000 long (>30 nts) conserved fragments at or close to upstream of the PAS were identified through remote species comparison. A substantial portion of them are longer than 100 nts, which is much longer than any known RNA protein recognition sites. A PAS classifier was built using logistic regression in order to study the characteristics of PAS. Not only it does improve the computational recognition of mammalian PAS than existing methods, it is also helpful in identifying a small number of genes that lack of typical PAS characteristics such as the poly(A) signal and/or the U/GU rich region. These findings provide useful experimental candidates for the study of the still unclear polyadenylation compensatory and/or regulatory elements. At present, no sequence consensus has been identified for the downstream U/GU enriched region yet. Thus, I have designed a novel rule-based nucleotide sequence motif finding algorithm, called iTriplet, to target long and degenerative motifs with special attention to the PAS downstream sequence. iTriplet has been demonstrated to handle motifs longer than 20 nts, which is still a challenge to existing methods. The utility of iTriplet has been confirmed by showing it accurately predicts PAS downstream elements using a dual Luciferase reporter assay.
