Characterization and Nature of Seed Transmission of Seed Borne Virus Causing Mosaic of Cowpea (Vigna Unguiculata (L.) Walp.) PDF Download
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Author: SUMANA
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
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Book Description
Author: SUMANA
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
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Book Description
Author: Monilola Arinola Taiwo
Publisher:
ISBN:
Category : Beans
Languages : en
Pages : 108
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Book Description
Author: Muhammad Bashir
Publisher:
ISBN:
Category : Cowpea
Languages : en
Pages : 444
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Book Description
Author: Lamptey, Paul N. L
Publisher: 1972 [c1973]
ISBN:
Category :
Languages : en
Pages : 400
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Book Description
Author: K. Subramanya Sastry
Publisher: Springer Science & Business Media
ISBN: 8132208137
Category : Medical
Languages : en
Pages : 343
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Book Description
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.
Author: B. Mahalakshmi
Publisher:
ISBN:
Category :
Languages : en
Pages : 92
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Book Description
Author: SANJEEVA REDDY H
Publisher:
ISBN:
Category :
Languages : en
Pages : 88
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Book Description
![Identification of Genotypes and Tagging of Available SSR Markers Linked to BCMV (Bean Common Mosaic Virus) Resistance in Early Segregating Generation of Cowpea [Vigna Unguiculata (L.) Walp.] and Evaluation of Nature of Seed Transmission PDF](https://books.google.com/books/content/images/frontcover/kmxqswEACAAJ?fife=w80-h100)
Author: P. Aishwarya
Publisher:
ISBN:
Category :
Languages : en
Pages : 80
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Book Description
Author: B.H Sunitha
Publisher:
ISBN:
Category :
Languages : en
Pages : 105
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Book Description
Author: Jody Jellison Goodell
Publisher:
ISBN:
Category : Lentils
Languages : en
Pages : 250
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Book Description
The lentil strain of pea seedborne mosaic virus, PSbMV-L, was distinguished from the standard isolates of PSbMV by a natural inoculum reservoir in lentil seed and by separate resistance genes in Pisum and Lens. PSbMV-L was intermediate in symptom severity between the standard and New Zealand isolates of PSbMV. Seed transmission rates up to 16.7% were observed for PSbMV-L in seed increase plots of infected lentil accessions, but the virus was not detected in commercial lentil plantings. The virus was nonpersistently transmitted by Acyrthosiphon pisum. Maximum rates of aphid transmission occurred with acquisition access periods of 3 to 5 minutes and ranged from 80 to 100%. Aphid transmission rates decreased with increasing temperature. Apparent trimodal transmission of PSbMV-L was observed. PSbMV-L is characterized by intrinsic particle instability. Partial purification was obtained by extracting infected lentil leaves in a neutral buffer, clarification with 7.5% chloroform, centrifugation through PEG (6 %)- sucrose (35%) cushions and equilibrium centrifugation in CsC1 gradients. The absorbance ratio 260/280 was 1.15. PSbMV-SL25 and PSbMV-L were indistinguishable on the basis of protein subunit molecular weight and could not be differentiated using classical serological techniques. The strains could be separated by quantitative ELISA using polyclonal sera produced in rabbit or chicken. Attempts to achieve greater antibody specificity were made with spleen cells from previously immunized mice that were fused with myoloma cells to create antibody-secreting hybridomas. The resultant colonies were screened using a modified ELISA against PSbMV-SL25 and PSbMV-L for the presence of specific antibody production. Selected colonies were expanded. The molecular weight of PSbMV-L RNA was estimated at 3.5 million. DNA complementary to PSbMV-C4 RNA was prepared using the random primer method of Taylor et al. Hybridization kinetics with PSbMV-L, PSbMV-SL25 and BYMV-Gil RNA were studied using Sl-nuclease assays. The lentil strain showed significantly lower levels of hybridization than the homologous control. Heterologous hybridization was severely decreased when the stringency of the assay conditions was increased.
