Characterisation of Neonicotinoid Resistance in the Cotton Aphid, Aphis Gossypii from Australian Cotton

Characterisation of Neonicotinoid Resistance in the Cotton Aphid, Aphis Gossypii from Australian Cotton PDF Author: Kate L. Langfield
Publisher:
ISBN:
Category : Cotton
Languages : en
Pages : 0

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Book Description
The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a highly polyphagous pest that inflicts serious damage to a broad range of agricultural, horticultural and greenhouse crops. In Australia, A. gossypii is a significant pest of cotton and is difficult to control with insecticides because of its high propensity to develop resistance. Neonicotinoids are among the most effective insecticides used to control A. gossypii but the most recent detection of resistance threatens their longevity. Consequently, I aimed to restore neonicotinoid efficacy against A. gossypii through elucidation of underlying resistance mechanism(s). Bioassay was used to measure thiamethoxam (neonicotinoid) response in three field strains collected from commercial cotton. A second trial investigated the use of phorate (an organophosphate) as an alternative pre-germination treatment to thiamethoxam. PCR-Sequencing was employed to identify if mutation R81T known to confer resistance to neonicotinoid compounds was present in Australian A. gossypii. High-throughput sequencing of the A. gossypii transcriptome found differences in gene expression associated wit hthiamethoxam resistance. Two transcripts involved in the detoxification of xenobiotics (putatively annotated as cytochrome p450 gene 6K1-like) were found differentially expressed between resistant and suceptible strains. Transcript expression was further validated by qRT-PCR and showed a similar tendency in upregulation of expression. As such I identified this gene as the strongest candidate for thiamethoxam resistant A. gossypii. This study has generated a comprehensive transcriptome resource for A. gossypii that has characterised the expression of numerous important transcripts encoding proteins involved in insecticide resistance. Consequently, my study will contribute to future research relating to molecular characterization of insecticide resistance mechanisms in A. gossypii and other insect pests.

Characterisation of Neonicotinoid Resistance in the Cotton Aphid, Aphis Gossypii from Australian Cotton

Characterisation of Neonicotinoid Resistance in the Cotton Aphid, Aphis Gossypii from Australian Cotton PDF Author: Kate L. Langfield
Publisher:
ISBN:
Category : Cotton
Languages : en
Pages : 0

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Book Description
The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a highly polyphagous pest that inflicts serious damage to a broad range of agricultural, horticultural and greenhouse crops. In Australia, A. gossypii is a significant pest of cotton and is difficult to control with insecticides because of its high propensity to develop resistance. Neonicotinoids are among the most effective insecticides used to control A. gossypii but the most recent detection of resistance threatens their longevity. Consequently, I aimed to restore neonicotinoid efficacy against A. gossypii through elucidation of underlying resistance mechanism(s). Bioassay was used to measure thiamethoxam (neonicotinoid) response in three field strains collected from commercial cotton. A second trial investigated the use of phorate (an organophosphate) as an alternative pre-germination treatment to thiamethoxam. PCR-Sequencing was employed to identify if mutation R81T known to confer resistance to neonicotinoid compounds was present in Australian A. gossypii. High-throughput sequencing of the A. gossypii transcriptome found differences in gene expression associated wit hthiamethoxam resistance. Two transcripts involved in the detoxification of xenobiotics (putatively annotated as cytochrome p450 gene 6K1-like) were found differentially expressed between resistant and suceptible strains. Transcript expression was further validated by qRT-PCR and showed a similar tendency in upregulation of expression. As such I identified this gene as the strongest candidate for thiamethoxam resistant A. gossypii. This study has generated a comprehensive transcriptome resource for A. gossypii that has characterised the expression of numerous important transcripts encoding proteins involved in insecticide resistance. Consequently, my study will contribute to future research relating to molecular characterization of insecticide resistance mechanisms in A. gossypii and other insect pests.

Insecticide Resistance Management in Cotton Aphid (Aphis Gossypii) and Cotton Mite (Tetranychus Urticae)

Insecticide Resistance Management in Cotton Aphid (Aphis Gossypii) and Cotton Mite (Tetranychus Urticae) PDF Author: Grant Alexander Herron
Publisher:
ISBN: 9780734716767
Category : Cotton
Languages : en
Pages : 34

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Book Description


Insecticide Resistance in Cotton Aphid

Insecticide Resistance in Cotton Aphid PDF Author: Grant Alexander Herron
Publisher:
ISBN: 9780731057368
Category : Cotton
Languages : en
Pages : 15

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Book Description


Sticky Cotton

Sticky Cotton PDF Author: Eric F. Hequet
Publisher: Texas Tech University Press
ISBN: 9780896725904
Category : Reference
Languages : en
Pages : 208

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Book Description
An essential reference for anyone searching for ways to avoid or mitigate the problem of cotton stickiness.

Aphids as Crop Pests, 2nd Edition

Aphids as Crop Pests, 2nd Edition PDF Author: Helmut F van Emden
Publisher: CABI
ISBN: 1780647093
Category : Technology & Engineering
Languages : en
Pages : 716

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Book Description
Aphids are among the major global pest groups, causing serious economic damage to many food and commodity crops in most parts of the world. This revision and update of the well-received first edition published ten years ago reflects the expansion of research in genomics, endosymbionts and semiochemicals, as well as the shift from control of aphids with insecticides to a more integrated approach imposed by increasing resistance in the aphids and government restrictions on pesticides. The book remains a comprehensive and up-to-date reference work on the biology of aphids, the various methods of controlling them and the progress of integrated pest management as illustrated by ten case histories.

Integration of Insect-Resistant Genetically Modified Crops within IPM Programs

Integration of Insect-Resistant Genetically Modified Crops within IPM Programs PDF Author: Jörg Romeis
Publisher: Springer Science & Business Media
ISBN: 1402083734
Category : Technology & Engineering
Languages : en
Pages : 451

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Book Description
Insect pests remain one of the main constraints to food and fiber production worldwide despite farmers deploying a range of techniques to protect their crops. Modern pest control is guided by the principles of integrated pest management (IPM) with pest resistant germplasm being an important part of the foundation. Since 1996, when the first genetically modified (GM) insect-resistant maize variety was commercialized in the USA, the area planted to insect-resistant GM varieties has grown dramatically, representing the fastest adoption rate of any agricultural technology in human history. The goal of our book is to provide an overview on the role insect-resistant GM plants play in different crop systems worldwide. We hope that the book will contribute to a more rational debate about the role GM crops can play in IPM for food and fiber production.

Sucking Pests of Crops

Sucking Pests of Crops PDF Author: Omkar
Publisher: Springer Nature
ISBN: 9811561494
Category : Science
Languages : en
Pages : 515

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Book Description
Sucking pests are most notorious group of pests for agricultural crops. Unlike most pests with chewing mouth parts, sucking pests cause more severe damage to the crops and are complex to get identified until advanced stages of infection. Not only is this late detection detrimental to their effective control, sucking pests also often cause fungal growth and virus transmission. The book emphasizes on sucking pests of most major crops of India. It aims to reflect Indian scenario before the international readership. This book complies comprehensive information on sucking pests of crops and brings the attention of the readers to this multiple damage causing insect complex. The chapters are contributed by highly experienced Indigenous experts from Universities & ICAR institutes, and book collates useful content for students and young researchers in plant pathology, entomology and agriculture.

Modern Crop Protection Compounds

Modern Crop Protection Compounds PDF Author: Peter Jeschke
Publisher: John Wiley & Sons
ISBN: 3527340890
Category : Science
Languages : en
Pages : 1772

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Book Description
The leading reference on this topic has just gotten better. Building on the success of the previous two editions, all the chapters have been updated to reflect the latest developments in the field, and new chapters have been added on picolinic acids, oxathiapiprolin, flupyradifurone, and other topics. This third edition presents the most important active ingredients of modern agrochemicals, with one volume each for herbicides, fungicides, and insecticides. The international team of first-class authors from such renowned crop science companies as Bayer, Syngenta, Dow AgroSciences, DuPont (now Corteva Agriscience), and BASF, address all crucial aspects from the general chemistry and the mode of action to industrial-scale synthesis, as well as from the development of products and formulations to their application in the field. A comprehensive and invaluable source of timely information for all of those working in modern biology, including genetics, biochemistry and chemistry, and for those in modern crop protection science, whether governmental authorities, researchers in agrochemical companies, scientists at universities, conservationists, or managers in organizations and companies involved in improvements to agricultural production.

Insect Evolutionary Ecology

Insect Evolutionary Ecology PDF Author: Royal Entomological Society of London. Symposium
Publisher: CABI
ISBN: 9781845931407
Category : Electronic books
Languages : en
Pages : 568

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Book Description
Insects provide excellent model systems for understanding evolutionary ecology. They are abundant, small, and relatively easy to rear, and these traits facilitate both field and laboratory experiments. This book has been developed from the Royal Entomological Society's 22nd international symposium, held in Reading in 2003. Topics include speciation and adaptation; life history, phenotype plasticity and genetics; sexual selection and reproductive biology; insect-plant interactions; insect-natural enemy interactions; and social insects.

General Concepts in Integrated Pest and Disease Management

General Concepts in Integrated Pest and Disease Management PDF Author: A. Ciancio
Publisher: Springer Science & Business Media
ISBN: 1402060610
Category : Science
Languages : en
Pages : 366

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Book Description
This, the first volume of the ‘Integrated Management of Plant Pests and Diseases’ book series, presents general concepts on integrated pest and disease management. Section one includes chapters on infection models, resurgence and replacement, plant disease epidemiology and effects of climate change in tropical environments. The second section includes remote sensing and information technology. Finally, the third section covers molecular aspects of the subject.