Capillary Electrophoresis of DNA in Uncrosslinked Polymer Solutions PDF Download
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Author: Annelise Emily Barron
Publisher:
ISBN:
Category :
Languages : en
Pages : 704
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Book Description
Author: Annelise Emily Barron
Publisher:
ISBN:
Category :
Languages : en
Pages : 704
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Book Description
Author: Cheuk Wai Kan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Capillary electrophoresis (CE) in the presence of entangled, uncrosslinked polymer solutions is the dominant technology for several important genetic analyses that rely upon the size-based separation of DNA fragments. There is a growing interest in transforming the current CE technology into a microfabricated format for DNA sequencing and genotyping, for higher throughput and lower cost. This research aims at designing high-performance thermo-responsive polymers and copolymers of acrylamide derivatives for DNA sequencing and genotyping and at applying our prior knowledge in CE-based DNA analysis to a microchip-based method. Recent advances in DNA sequencing and genotyping, in miniaturized electrophoresis systems are critically reviewed. Polymers and copolymers of N-ethoxyethylacrylamide and N-methoxyethylacrylamide were synthesized, the polymer physical properties were carefully investigated, and the DNA sequencing performances of these polymer matrices were evaluated. Rheometry results suggest that these polymers undergo a sol-gel transition from viscous solutions to elastic, solid-like materials upon heating. DNA sequencing studies indicate that the physical stability brought about by thermo-gelation enhances single-stranded DNA separation resolution and hence extends the DNA sequencing read length relative to a non-thermogelling control matrix. A different approach to enhancing double-stranded DNA separation resolution was taken in an investigation using blends of two well-studied cellulose derivatives, hydroxyethylcellulose and hydroxypropylcellulose, to create a DNA sieving matrix with a thermally tunable mesh size for dsDNA separation. The thermo-responsive constituent of this polymer blend (hydroxypropylcellulose) allows the mesh size of the sieving matrix to be thermally controlled, to facilitate the optimal separation of DNA fragments over different size ranges, which is not easily attainable with conventional polymer sieving matrices. The approach was extended to blends of thermo-responsive N,N-diethylacrylamide and N,N-dimethylacrylamide copolymers. (Abstract shortened by UMI.)
Author: Keith R. Mitchelson
Publisher: Springer Science & Business Media
ISBN: 1592590551
Category : Science
Languages : en
Pages : 481
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Book Description
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Author: Chunhung Wu
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 322
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Author: Wade Michael Sunada
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 390
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Author: Christoph Heller
Publisher: Springer Science & Business Media
ISBN: 3322910156
Category : Technology & Engineering
Languages : en
Pages : 318
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Book Description
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 20
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Book Description
Because the mechanism of DNA separation in capillary electrophoresis is not well understood, selection of polymers is a {open_quotes}trial-and-error{close_quotes} procedure. We investigated dilute-solution DNA separations by capillary electrophoresis using solutions of four polymers that differ in size, shape and stiffness. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp and star-(polyethylene oxide), like linear poly (ethylene oxide), provides separation of fragments up to 1353 bp.
Author: Patrick Camilleri
Publisher: CRC Press
ISBN: 9780849391279
Category : Medical
Languages : en
Pages : 586
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Book Description
The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.
Author: Andras Guttman
Publisher: Newnes
ISBN: 0080931359
Category : Science
Languages : en
Pages : 391
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Book Description
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author: George D. J. Phillies
Publisher: Cambridge University Press
ISBN: 1139504517
Category : Science
Languages : en
Pages : 527
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Book Description
Presenting a completely new approach to examining how polymers move in non-dilute solution, this book focuses on experimental facts, not theoretical speculations, and concentrates on polymer solutions, not dilute solutions or polymer melts. From centrifugation and solvent dynamics to viscosity and diffusion, experimental measurements and their quantitative representations are the core of the discussion. The book reveals several experiments never before recognized as revealing polymer solution properties. A novel approach to relaxation phenomena accurately describes viscoelasticity and dielectric relaxation and how they depend on polymer size and concentration. Ideal for graduate students and researchers interested in the properties of polymer solutions, the book covers real measurements on practical systems, including the very latest results. Every significant experimental method is presented in considerable detail, giving unprecedented coverage of polymers in solution.
