Author: Jennifer A. Doudna
Publisher:
ISBN: 9781621821304
Category : Genetics
Languages : en
Pages : 0
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Book Description
CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Author: Carlos F. Barbas
Publisher: CSHL Press
ISBN: 0879697407
Category : Science
Languages : en
Pages : 724
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Book Description
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Author: St. Marys Hospital Medical Center
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 498
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Author:
Publisher:
ISBN:
Category : Education
Languages : en
Pages : 344
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Book Description
Author: William J. Wallace
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 264
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Book Description
Author: Hazel L. Sive
Publisher: CSHL Press
ISBN: 9780879695040
Category : Medical
Languages : en
Pages : 370
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Book Description
Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 724
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Book Description
Author: Johnny Saldana
Publisher: SAGE
ISBN: 1446200124
Category : Reference
Languages : en
Pages : 282
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Book Description
The Coding Manual for Qualitative Researchers is unique in providing, in one volume, an in-depth guide to each of the multiple approaches available for coding qualitative data. In total, 29 different approaches to coding are covered, ranging in complexity from beginner to advanced level and covering the full range of types of qualitative data from interview transcripts to field notes. For each approach profiled, Johnny Saldaña discusses the method’s origins in the professional literature, a description of the method, recommendations for practical applications, and a clearly illustrated example.
Author: The Modern Language Association of America
Publisher: Modern Language Association
ISBN: 1603293523
Category : Language Arts & Disciplines
Languages : en
Pages : 339
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Book Description
Relied on by generations of writers, the MLA Handbook is published by the Modern Language Association and is the only official, authorized book on MLA style. The new, ninth edition builds on the MLA's unique approach to documenting sources using a template of core elements--facts, common to most sources, like author, title, and publication date--that allows writers to cite any type of work, from books, e-books, and journal articles in databases to song lyrics, online images, social media posts, dissertations, and more. With this focus on source evaluation as the cornerstone of citation, MLA style promotes the skills of information and digital literacy so crucial today. The many new and updated chapters make this edition the comprehensive, go-to resource for writers of research papers, and anyone citing sources, from business writers, technical writers, and freelance writers and editors to student writers and the teachers and librarians working with them. Intended for a variety of classroom contexts--middle school, high school, and college courses in composition, communication, literature, language arts, film, media studies, digital humanities, and related fields--the ninth edition of the MLA Handbook offers New chapters on grammar, punctuation, capitalization, spelling, numbers, italics, abbreviations, and principles of inclusive language Guidelines on setting up research papers in MLA format with updated advice on headings, lists, and title pages for group projects Revised, comprehensive, step-by-step instructions for creating a list of works cited in MLA format that are easier to learn and use than ever before A new appendix with hundreds of example works-cited-list entries by publication format, including websites, YouTube videos, interviews, and more Detailed examples of how to find publication information for a variety of sources Newly revised explanations of in-text citations, including comprehensive advice on how to cite multiple authors of a single work Detailed guidance on footnotes and endnotes Instructions on quoting, paraphrasing, summarizing, and avoiding plagiarism A sample essay in MLA format Annotated bibliography examples Numbered sections throughout for quick navigation Advanced tips for professional writers and scholars
Author:
Publisher:
ISBN:
Category : Power resources
Languages : en
Pages : 622
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Book Description
