Biochemical Studies on the Role of RNA Polymerase II Phosphorylation During Transcription Initiation PDF Download
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Author: Hua Lü
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Author: Hua Lü
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Author: Jonathan Donald Chesnut
Publisher:
ISBN:
Category :
Languages : en
Pages : 280
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Author: Sharon Lynn Wampler
Publisher:
ISBN:
Category :
Languages : en
Pages : 170
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Author: Mark E. Samuels
Publisher:
ISBN:
Category :
Languages : en
Pages : 424
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Author: Cai Huang
Publisher: BoD – Books on Demand
ISBN: 9535107372
Category : Medical
Languages : en
Pages : 482
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Book Description
15 chapters on protein phosphorylation and human health written by expert scientists. Covers most important research hot points, such as Akt, AMPK and mTOR. Bridges the basic protein phosphorylation pathways with human health and diseases. Detailed and comprehensive text with excellent figure illustration.
Author: Peter Hsu
Publisher:
ISBN:
Category :
Languages : en
Pages : 118
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Book Description
RNA Polymerase II (PolII) dependent transcription of mRNAs is central to gene expression throughout eukaryotes. Transcription is a highly regulated process, with a defined initiation, elongation, and termination phase, all of which are controlled by multiple trans-acting protein factors and cis-acting elements on the template DNA and transcribed RNA. Extensive work has shown that the phosphorylation state of the C- terminal domain (CTD) of PolII plays a central role in the recruitment of trans-acting factors during all phases of transcription. Aberrant phosphorylation can result in a lethal phenotype in yeasts, therefore implying that correct control of phosphorylation by kinases and phosphatases specific for the CTD is critical for life. In addition to the polymerase, conserved cis-acting sequence elements near the 3'-end on pre-mRNAs help to define and recruit various RNA processing machines to the transcription elongation complex in order to properly process and package the pre-mRNA for export to the cytoplasm for translation. In this thesis, I first review current knowledge regarding PolII CTD phosphorylation and its effects on the transcription elongation complex. Additionally, in this first chapter, I will also provide an overview of the 3'-end mRNA processing/transcription termination machinery. In the second part of my thesis, I will describe my doctoral work on the structural and biochemical characterization of Rtr1, a unique PolII CTD phosphatase that represents a novel new member of this class of enzymes. In my studies, I show that Rtr1 is a bona fide phosphatase of unique sequence and structure that is allosterically regulated by its own C-terminus. Additionally, I show that Rtr1 is a dual specificity phosphatase, with activities against both serine and tyrosine residues on the CTD. In chapter 3 of this thesis, I describe my work on the in vitro reconstitution of the Cleavage Stimulation Factor (CstF) responsible for the recognition of sequences downstream of the polyadenylation site on pre-mRNAs that help to define the 3'-end processing reaction that occur at the end of genes. Using highly purified proteins, I show for the first time that CstF is a dimer of trimers, with two copies of each subunit in the entire assembly. In addition, I show that CstF, as a complex, can bind to G/U rich RNAs with nanomolar affinities, in stark contrast with previous studies showing that singly purified proteins from the complex binding with much weaker affinities.
Author: David C. Amberg
Publisher: CSHL Press
ISBN: 0879697288
Category : Genetics
Languages : en
Pages : 250
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Book Description
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Author: Sankar Adhya
Publisher: Academic Press
ISBN:
Category : Medical
Languages : en
Pages : 416
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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Promoter elements and RNA polymerase components * RNA polymerase and its subunits in prokaryotes * Biochemical assays of transcription initiation * RNA polymerase and associated factors from eukaryotes * Genetic analysis of transcription and its regulation
Author: Ronald C. Conaway
Publisher: Raven Press (ID)
ISBN:
Category : Science
Languages : en
Pages : 600
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Book Description
Presents a coherent account of many productive lines of investigation, organized as a series of mini-reviews that focus on major research areas including studies on the structure and mechanisms of action of bacterial, viral, and eukaryotic RNA polymerases, and the transcription factors that control their activities. Each review provides a brief but up-to-date account of the progress of research in a particular area, a discussion of the major issues and questions driving that research, and a brief description of the evolving approaches and technologies used to address those questions. Annotation copyright by Book News, Inc., Portland, OR
Author: Chun-hsiang Chang
Publisher:
ISBN:
Category : RNA polymerases
Languages : en
Pages : 336
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