Analytical Ultracentrifugation in Biochemistry and Polymer Science PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Analytical Ultracentrifugation in Biochemistry and Polymer Science PDF full book. Access full book title Analytical Ultracentrifugation in Biochemistry and Polymer Science by Stephen E. Harding. Download full books in PDF and EPUB format.
Author: Stephen E. Harding
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 656
Get Book Here
Book Description
This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.
Author: Stephen E. Harding
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 656
Get Book Here
Book Description
This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.
Author: David Scott
Publisher: Royal Society of Chemistry
ISBN: 1847552617
Category : Science
Languages : en
Pages : 613
Get Book Here
Book Description
Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.
Author: Susumu Uchiyama
Publisher: Springer
ISBN: 443155985X
Category : Science
Languages : en
Pages : 520
Get Book Here
Book Description
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.
Author: Todd M. Schuster
Publisher: Springer Science & Business Media
ISBN: 1468468286
Category : Science
Languages : en
Pages : 359
Get Book Here
Book Description
There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.
Author: Walter Maechtle
Publisher: Springer Science & Business Media
ISBN: 3540262180
Category : Technology & Engineering
Languages : en
Pages : 249
Get Book Here
Book Description
This book is divided into chapters covering instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
Author:
Publisher: Academic Press
ISBN: 0128029099
Category : Science
Languages : en
Pages : 617
Get Book Here
Book Description
Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals. - Timely contribution that describes a rapidly changing field - Leading researchers in the field - Broad coverage: instrumentation, basic theory, data analysis, and applications
Author: Peter Schuck
Publisher: CRC Press
ISBN: 1351976834
Category : Science
Languages : en
Pages : 227
Get Book Here
Book Description
Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles, and the experimental and instrumental aspects in the first volume Basic Principles of Analytical Ultracentrifugation, the present volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. This volume is designed to fill a gap in biophysical methodology to provide a framework that builds on the fundamentals of the highly developed traditional methods of analytical ultracentrifugation, updated with current methodology and from a viewpoint of modern applications. It will be an invaluable resource for researchers and graduate students interested in the application of analytical ultracentrifugation in the study of interacting systems, such as biological macromolecules, multi-protein complexes, polymers, or nanoparticles.
Author: G. Allen
Publisher: Elsevier
ISBN: 0080551335
Category : Science
Languages : en
Pages : 329
Get Book Here
Book Description
In the first volume of this series, the structures of protein molecules were described, together with computational methods linking sequence data to folded structure and function. The determination of protein structure by nuclear magnetic resonance spectroscopy was also presented. Volume 2 begins by continuing the theme of protein structure with an outline of methods of crystallographic structure determination. Subsequent chapters describe various strucure-related properties of proteins. This book provides a valuable contribution to those new to the field of protein science as well as to those already expert in the field.
Author: David E. Metzler
Publisher: Elsevier
ISBN: 0080924700
Category : Science
Languages : en
Pages : 968
Get Book Here
Book Description
The most comprehensive textbook/reference ever to cover the chemical basis of life, the "Green Bible of Biochemistry" has been a well-respected contribution to the field for more than twenty years. The complex structures that make up cells are described in detail, along with the forces that hold them together, and the chemical reactions that allow for recognition, signaling and movement. There is ample information on the human body, its genome, and the action of muscles, eyes, and the brain. The complete set deals with the natural world, treating the metabolism of bacteria, toxins, antibiotics, specialized compounds made by plants, photosynthesis, luminescence of fireflies, among many other topics.* The most comprehensive biochemistry text reference available on the market* Organized into two volumes, comprising 32 chapters and containing the latest research in the field* Biological content is emphasized: for example, macromolecular structures and enzyme action are discussed
Author: Vladimir N. Uversky
Publisher: Nova Publishers
ISBN: 9781600217043
Category : Science
Languages : en
Pages : 414
Get Book Here
Book Description
Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
