ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. PDF Download
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Author: Oleksandr Kirsanov
Publisher:
ISBN:
Category :
Languages : en
Pages : 92
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Book Description
Spermatogenesis in the mammalian testis results in the daily production of millions of spermatozoa. This developmental process is founded upon the actions of a small population of spermatogonial stem cells (SSCs). As SSCs divide, their progeny must either remain an SSC to maintain the stem cell pool (self-renewal) or become an undifferentiated progenitor spermatogonium that proliferates prior to differentiating in response to retinoic acid (RA) and entering meiosis. Our laboratory is focused on understanding the mechanisms that regulate these fate decisions as well as defining the changes that occur downstream of RA that prepare spermatogonia for entry into meiosis. Currently, researchers have been unable to readily isolate pure populations of spermatogonia in the developing or adult testis. To address this, I have examined multiple transgenic mouse models to identify those with germ cell-specific expression of fluorescent reporter genes that will enable us to isolate spermatogonia using fluorescence-activated cell sorting (FACS). The results in this thesis describe these efforts, and document the identification of an excellent mouse model for future studies in our laboratory to understand the mechanisms underlying spermatogonial development. This work was supported by a grant from the NIH/NICHD (HD090083) to C.B.G.
Author: Oleksandr Kirsanov
Publisher:
ISBN:
Category :
Languages : en
Pages : 92
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Book Description
Spermatogenesis in the mammalian testis results in the daily production of millions of spermatozoa. This developmental process is founded upon the actions of a small population of spermatogonial stem cells (SSCs). As SSCs divide, their progeny must either remain an SSC to maintain the stem cell pool (self-renewal) or become an undifferentiated progenitor spermatogonium that proliferates prior to differentiating in response to retinoic acid (RA) and entering meiosis. Our laboratory is focused on understanding the mechanisms that regulate these fate decisions as well as defining the changes that occur downstream of RA that prepare spermatogonia for entry into meiosis. Currently, researchers have been unable to readily isolate pure populations of spermatogonia in the developing or adult testis. To address this, I have examined multiple transgenic mouse models to identify those with germ cell-specific expression of fluorescent reporter genes that will enable us to isolate spermatogonia using fluorescence-activated cell sorting (FACS). The results in this thesis describe these efforts, and document the identification of an excellent mouse model for future studies in our laboratory to understand the mechanisms underlying spermatogonial development. This work was supported by a grant from the NIH/NICHD (HD090083) to C.B.G.
Author: Jeffrey E. Green
Publisher: Springer Science & Business Media
ISBN: 0387698051
Category : Medical
Languages : en
Pages : 639
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Book Description
Genetically-engineered mouse models for cancer research have become invaluable tools for studying cancer biology and evaluating novel therapeutic approaches. This volume focuses on state-of-the-art methods for generating, analyzing and validating such models for studying aspects of human cancer biology. Additionally, these models are emerging as important pre-clinical systems in which to test cancer prevention and therapeutic strategies in order to select compounds for testing in clinical trials.
Author: Marten H. Hofker
Publisher: Springer Science & Business Media
ISBN: 1592593402
Category : Science
Languages : en
Pages : 382
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Book Description
Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.
Author: Martin Matzuk
Publisher: American Mathematical Soc.
ISBN: 9780896037649
Category : Medical
Languages : en
Pages : 500
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Book Description
A panel of expert clinical and basic investigators summarize the state-of-the-art in the use of transgenic technology in a broad range of endocrinological applications. Up-to-date and comprehensive, Transgenics in Endocrinology reviews the most recent developments in the analysis of endocrine physiology and its pathologies through mouse models, and provides a host of creative approaches to understand better the complex processes that are characteristic of hormonal systems.
Author: P.H. Vogt
Publisher: Karger Medical and Scientific Publishers
ISBN: 3318060984
Category : Medical
Languages : en
Pages : 190
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Book Description
Infertility affects more than one in ten couples worldwide and is related to highly heterogeneous pathologies sometimes only discernible in the germ line. Its complex etiology often, but not always, includes genetic factors besides anatomical defects, immunological interference, and environmental aspects. Nearly 30% of infertility cases are probably caused only by genetic defects. Thereby experimental animal knockout models convincingly show that infertility can be caused by single or multiple gene defects. Translating those basic research findings into clinical studies is challenging, leaving genetic causes for the vast majority of infertility patients unexplained. Nevertheless, a large number of candidate genes have been revealed by sophisticated molecular methods. This book provides a comprehensive overview on the subject of infertility written by the leading authorities in this field. It covers topics including basic biological, cytological, and molecular studies, as well as common and uncommon syndromes. It is a must-read for human geneticists, endocrinologists, epidemiologists, zoologists, and counsellors in human genetics, infertility, and assisted reproduction.
Author: Shree Ram Singh
Publisher: Humana
ISBN: 9781493945672
Category : Medical
Languages : en
Pages : 0
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Book Description
Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls. Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.
Author: Christopher J. De Jonge
Publisher: Cambridge University Press
ISBN: 9780521853972
Category : Medical
Languages : en
Pages : 372
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Book Description
Originally published in 2006, this is a comprehensive and definitive account of the human male gamete. The volume summarizes many unique and revealing characteristics of the sperm cell. It provides a detailed overview of human sperm production, maturation and function, and looks at how these processes affect and influence fertility, infertility and ART. The volume thus provides a detailed review of the most important research and developments, augmented with pertinent references. This book will appeal to all practitioners and scientists in reproductive medicine and in particular to clinical scientists, graduate and post-graduate scientists, and laboratory personnel.
Author: Katsunori Tanaka
Publisher: John Wiley & Sons
ISBN: 3527344322
Category : Science
Languages : en
Pages : 560
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Book Description
Provides timely, comprehensive coverage of in vivo chemical reactions within live animals This handbook summarizes the interdisciplinary expertise of both chemists and biologists performing in vivo chemical reactions within live animals. By comparing and contrasting currently available chemical and biological techniques, it serves not just as a collection of the pioneering work done in animal-based studies, but also as a technical guide to help readers decide which tools are suitable and best for their experimental needs. The Handbook of In Vivo Chemistry in Mice: From Lab to Living System introduces readers to general information about live animal experiments and detection methods commonly used for these animal models. It focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release. Topics include: currently available mouse models; biocompatible fluorophores; radionuclides for radiodiagnosis/radiotherapy; live animal imaging techniques such as positron emission tomography (PET) imaging; magnetic resonance imaging (MRI); ultrasound imaging; hybrid imaging; biocompatible chemical reactions; ligand-directed nucleophilic substitution chemistry; biorthogonal prodrug release strategies; and various selective targeting strategies for live animals. -Completely covers current techniques of in vivo chemistry performed in live animals -Describes general information about commonly used live animal experiments and detection methods -Focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release -Places emphasis on material properties required for the development of appropriate compounds to be used for imaging and therapeutic purposes in preclinical applications Handbook of In Vivo Chemistry in Mice: From Lab to Living System will be of great interest to pharmaceutical chemists, life scientists, and organic chemists. It will also appeal to those working in the pharmaceutical and biotechnology industries.
Author: Larry I. Lipshultz
Publisher: Cambridge University Press
ISBN: 1139483242
Category : Medical
Languages : en
Pages : 691
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Book Description
The new edition of this canonical text on male reproductive medicine will cement the book's market-leading position. Practitioners across many specialties - including urologists, gynecologists, reproductive endocrinologists, medical endocrinologists and many in internal medicine and family practice – will see men with suboptimal fertility and reproductive problems. The book provides an excellent source of timely, well-considered information for those training in this young and rapidly evolving field. While several recent books provide targeted 'cookbooks' for those in a male reproductive laboratory, or quick reference for practising generalists, the modern, comprehensive reference providing both a background for male reproductive medicine as well as clinical practice information based on that foundation has been lacking until now. The book has been extensively revised with a particular focus on modern molecular medicine. Appropriate therapeutic interventions are highlighted throughout.
Author: Lonnie Dee Russell
Publisher:
ISBN:
Category : Sertoli cells
Languages : en
Pages : 840
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Book Description
