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Author: Su-Wen Ho
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 157
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Book Description
Gene expression is an elaborate and finely tuned process involving the regulated interactions of multiple proteins with promoter and enhancer elements. A variety of approaches are currently used to study these interactions in vivo, in vitro as well as in silico. With the genome sequences of many organisms now readily available, a plethora of DNA functional elements have been predicted, but the process of identifying the proteins that bind to them in vivo remains a bottleneck. I developed two high-throughput assays to address this issue. The first is a modification of the yeast "one-hybrid" assay. The second is probing protein microarrays with DNA sequence elements. Using these methods, I identified two proteins, Sef1 and Yjl103c, that bind to the same DNA sequence element. Sef1 and Yjl103c are little-characterized members of the zinc cluster family of transcription factors of S. cerevisiae. Characterization of their mechanism of action as well as identification of some of their target genes leads to the conclusion that they play a pivotal role in the transcriptional regulation of utilization of nonfermentable carbon sources by budding yeast.
Author: Su-Wen Ho
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 157
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Book Description
Gene expression is an elaborate and finely tuned process involving the regulated interactions of multiple proteins with promoter and enhancer elements. A variety of approaches are currently used to study these interactions in vivo, in vitro as well as in silico. With the genome sequences of many organisms now readily available, a plethora of DNA functional elements have been predicted, but the process of identifying the proteins that bind to them in vivo remains a bottleneck. I developed two high-throughput assays to address this issue. The first is a modification of the yeast "one-hybrid" assay. The second is probing protein microarrays with DNA sequence elements. Using these methods, I identified two proteins, Sef1 and Yjl103c, that bind to the same DNA sequence element. Sef1 and Yjl103c are little-characterized members of the zinc cluster family of transcription factors of S. cerevisiae. Characterization of their mechanism of action as well as identification of some of their target genes leads to the conclusion that they play a pivotal role in the transcriptional regulation of utilization of nonfermentable carbon sources by budding yeast.
Author: Zhiping Liu
Publisher:
ISBN:
Category :
Languages : en
Pages : 248
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Book Description
Author: JOHANNA.. TIMMERMAN
Publisher:
ISBN:
Category :
Languages : fr
Pages : 290
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Book Description
DANS LA LEVURE SACCHAROMYCES CEREVISIAE, LA PROTEINE CYP1 EST UN REGULATEUR DE LA TRANSCRIPTION DE NOMBREUX GENES DONT L'EXPRESSION EST DEPENDANTE DE L'OXYGENE. NOUS NOUS SOMMES INTERESSE A LA DETERMINATION DE LA STRUCTURE TRIDIMENSIONNELLE DU DOMAINE DE FIXATION A L'ADN DE CETTE PROTEINE. CETTE PARTIE CONTIENT UN MOTIF CYS-XAA(2)-CYS-XAA(6)-CYS-XAA(6)-CYS-XAA(2)-CYS-XAA(8)-CYS, QUE L'ON RETROUVE DANS DE NOMBREUSES PROTEINES DE LEVURE INTERAGISSANT AVEC L'ADN, EN PARTICULIER DANS LA PROTEINE GAL4. LE BUT A LONG TERME EST DE DETERMINER LE COMPLEXE CYP1-ADN PAR RESONANCE MAGNETIQUE NUCLEAIRE. DANS CE TRAVAIL NOUS PRESENTONS LE CLONAGE ET L'EXPRESSION DANS ESCHERICHIA COLI DES QUATRE FRAGMENTS DIFFERENTS CORRESPONDANT A LA PARTIE N-TERMINALE DE L'ACTIVATEUR CYP1 RESPONSABLE DE LA FIXATION A L'ADN. LE FRAGMENT CYP1(49-126) A ETE CHOISI POUR UNE ETUDE STRUCTURALE PAR RESONANCE MAGNETIQUE NUCLEAIRE. POUR CE FRAGMENT, UN PROTOCOLE DE PURIFICATION A ETE MIS AU POINT. NOUS MONTRONS ENSUITE QUE LE ZINC EST UN ELEMENT ESSENTIEL DE LA STRUCTURE DE LA PROTEINE ET QUE LE FRAGMENT CYP1(49-126) POSSEDE 2 ATOMES DE ZINC. LES MEMES RESULTATS ONT ETE OBSERVES LORSQU'ON SUBSTITUE DEUX ATOMES DE ZINC PAR LES ATOMES CADMIUM 113. PAR RESONANCE MAGNETIQUE NUCLEAIRE, NOUS AVONS DEMONTRE QUE LES SIX CYSTEINES DU MOTIF LIGANDENT LES DEUX ATOMES DE CADMIUM. LE FRAGMENT A ETE ENSUITE MARQUE AVEC DE L'AZOTE 15. L'ENSEMBLE DES EXPERIENCES RMN HOMONUCLEAIRES A DEUX DIMENSIONS (COSY, TOCSY ET NOESY) ET HETERONUCLEAIRES A DEUX ET TROIS DIMENSIONS (HMQC, NOE-HMQC, HOHAHA-HMQC) A PERMIS L'ATTRIBUTION DE LA PARTIE N-TERMINALE DE CYP1(49-126). LES DONNEES EXPERIMENTALES OBTENUES PAR CES EXPERIENCES ONT ETE UTILISEES DANS DES PROGRAMMES DE MODELISATION, DIANA ET X-PLOR. UNE STRUCTURE TRIDIMENSIONNELLE DU FRAGMENT A PU ETRE PRESENTEE ET MONTRE QUE NOTRE FRAGMENT EST REPLIE EN CLUSTER A ZINC
Author: Christopher T. Harbison
Publisher:
ISBN:
Category :
Languages : en
Pages : 456
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Book Description
Historically, knowledge of gene-specific transcription has been accumulated by the study of the individual genetic and physical interactions between transcriptional regulators and the genes they regulate, often requiring considerable time and effort. Microarray technology now enables investigation of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a population of cells, as well as to locate the regions within the genome that are bound by transcriptional regulators. Such studies not only allow for the functional annotation of both genes and regulators, but can also provide clues about the identity of the regulatory regions within DNA, the structure of global regulatory networks and the regulation of DNA-binding proteins. These and other insights are presented here based on our genome-wide studies of transcriptional regulation in the yeast Saccharomyces cerevisiae.
Author: Warren David Kruger
Publisher:
ISBN:
Category : Genetic transcription
Languages : en
Pages : 402
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Author: Paul Wade
Publisher:
ISBN:
Category :
Languages : en
Pages : 324
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Book Description
Author: Julie Ayn Deardorff
Publisher:
ISBN:
Category :
Languages : en
Pages : 414
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Author: Jeffrey N. Strathern
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 776
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Author: Henri Heslot
Publisher: CRC Press
ISBN: 135108318X
Category : Technology & Engineering
Languages : en
Pages : 405
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Book Description
Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
Author: Kirk Tevebaugh Ehmsen
Publisher:
ISBN:
Category :
Languages : en
Pages : 938
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Book Description
