Agrobacterium-mediated Transformation of Brassica Oleracea Var. Italica and B. Napus with a Trichoderma Harzianum Endochitinase Gene to Enhance Resistance Against Fungal Pathogens PDF Download
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Author: Alejandra Mora-Avilʹes
Publisher:
ISBN:
Category : Agrobacterium tumefaciens
Languages : en
Pages : 474
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Book Description
"The control of pathogens in food crops is a general concern around the world. Much effort has been devoted to the control of fungal pathogens, mainly by pesticide application, cultural practices, biocontrol, or traditional plant breeding. Limited sources of resistance among cultivated plants or their wild relatives have made it important to look for alternative resistance genes and transfer techniques. This dissertation addresses the introduction of a Trichoderma. harzianum endochitinase gene into Brassica oleracea and B. napus by Agrobacterium tumefaciens-mediated transformation, and the molecular analysis and fungal resistance of the transgenic plants. The T. harzianum endochitinase has been reported to have fungal inhibiting properties in assays performed in vitro. Recently, transgenic crops such as potato, tomato, tobacco, apple, and grape expressing the endochitinase gene have been analyzed to assess their protection against fungal pathogens, with variable results. Chapter I reviews previous reports of chitinase genes from plants and microorganisms used in plant transformation. It describes the levels of expression of chitinase in various transgenic plants and the levels of control of fungal pathogens. Chapter I also discusses previous work on Agrobacterium-mediated transformation of Brassica oleracea. Chapter II presents experiments testing some of the factors that affect the efficiency of Agrobacterium-mediated transformation of broccoli. Chapter III focuses on molecular aspects of gene expression of transgenic broccoli and rapeseed plants containing the T. harzianum endochitinase gene and describes pathogenic assays to assess the resistance of the transgenic plants to the fungal pathogens Alternaria brassicicola and Sclerotinia sclerotiorum. Chapter IV discusses the interaction of endochitinase and the fungicide Bayleton for the control of A. brassicicola. Finally, Chapter V addresses the induction of pathogenesis-related proteins by constitutive expression of the endochitinase gene"--Leaves xv-xvi
Author: Alejandra Mora-Avilʹes
Publisher:
ISBN:
Category : Agrobacterium tumefaciens
Languages : en
Pages : 474
Get Book Here
Book Description
"The control of pathogens in food crops is a general concern around the world. Much effort has been devoted to the control of fungal pathogens, mainly by pesticide application, cultural practices, biocontrol, or traditional plant breeding. Limited sources of resistance among cultivated plants or their wild relatives have made it important to look for alternative resistance genes and transfer techniques. This dissertation addresses the introduction of a Trichoderma. harzianum endochitinase gene into Brassica oleracea and B. napus by Agrobacterium tumefaciens-mediated transformation, and the molecular analysis and fungal resistance of the transgenic plants. The T. harzianum endochitinase has been reported to have fungal inhibiting properties in assays performed in vitro. Recently, transgenic crops such as potato, tomato, tobacco, apple, and grape expressing the endochitinase gene have been analyzed to assess their protection against fungal pathogens, with variable results. Chapter I reviews previous reports of chitinase genes from plants and microorganisms used in plant transformation. It describes the levels of expression of chitinase in various transgenic plants and the levels of control of fungal pathogens. Chapter I also discusses previous work on Agrobacterium-mediated transformation of Brassica oleracea. Chapter II presents experiments testing some of the factors that affect the efficiency of Agrobacterium-mediated transformation of broccoli. Chapter III focuses on molecular aspects of gene expression of transgenic broccoli and rapeseed plants containing the T. harzianum endochitinase gene and describes pathogenic assays to assess the resistance of the transgenic plants to the fungal pathogens Alternaria brassicicola and Sclerotinia sclerotiorum. Chapter IV discusses the interaction of endochitinase and the fungicide Bayleton for the control of A. brassicicola. Finally, Chapter V addresses the induction of pathogenesis-related proteins by constitutive expression of the endochitinase gene"--Leaves xv-xvi
Author: Maurizio Vurro
Publisher: IOS Press
ISBN: 9781586032166
Category : Biological pest control agents
Languages : en
Pages : 308
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Book Description
Contains 24 contributions and an additional six abstracts focusing on the use of biocontrol agents in agriculture. Sections address the need for enhanced biocontrol agents and strategies for enhancement; technologies for enhancing biocontrol agents; risks from enhanced biocontrol agents and their mitigation; and genetics and molecular biology of enhancing biocontrol agents. The abstracts address emerging biocontrols including valine excreting isolate of Pseudomonas syringae cv. tagetis exhibiting enhanced virulence against hounds tongue (Cynoglossum officinale); application of fungal polysaccharides in plant protection; and the predisposing of forest weeds by chemical and manual cutting treatments to enhance the efficacy of selected biocontrol agents. Annotation copyrighted by Book News, Inc., Portland, OR.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 870
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Book Description
Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 848
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Book Description
Author: Chittaranjan Kole
Publisher: Springer Nature
ISBN: 3030758796
Category : Science
Languages : en
Pages : 340
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Book Description
This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the biotic stresses caused by different diseases and pests that are important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in cereal crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses. The eight chapters each dedicated to a cereal crop in this volume elucidate on different types of biotic stresses and their effects on and interaction with the crop; enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing biotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate on different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating biotic stress-resistant crops.
Author: Dinesh K. Maheshwari
Publisher: Springer Science & Business Media
ISBN: 3642210619
Category : Science
Languages : en
Pages : 352
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Book Description
The future of agriculture strongly depends on our ability to enhance productivity without sacrificing long-term production potential. An ecologically and economically sustainable strategy is the application of microorganisms, such as the diverse bacterial species of plant growth promoting bacteria (PGPB). The use of these bio-resources for the enhancement of crop productivity is gaining worldwide importance. “Bacteria in Agrobiology: Plant Nutrient Management” focus on the management of plant nutrient to support plant growth and development. The topics treated in this book include mechanisms of plant growth promoting rhizobacteria, zinc and phosphate solubilizing microorganisms, sulfur oxidizing bacteria, ACC deaminase, siderophores, phytohormones, quorum-sensing, biofilms, antibiotics, volatiles, denitrification and integrated nutrient management.
Author: Govind Singh Saharan
Publisher: Springer Nature
ISBN: 9811608628
Category : Science
Languages : en
Pages : 818
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Book Description
The book presents comprehensive information on fundamental, and applied knowledge for developing varieties resistant individually as well as to all the major pathogens of crucifers, such as Albugo, Alternaria, Erysiphe, Hyaloperonospora, Plasmodiophora, Leptosphaeria, Sclerotinia, Turnip mosaic virus, Verticillium, and Xanthomonas through the use of latest biotechnological approaches including identification of R genes and their incorporation into agronomically superior varieties. The chapters include the information’s viz., principles of host resistance, identification of R-genes sources, inheritance of disease resistance, host resistance signaling network system to multiple stresses. The book also covers transfer of disease resistance, and management of disease resistance. Standardized, reproducible techniques are also included for the researchers of cruciferous crops for developing resistant cultivars. The book deals with the gaps in understanding, knowledge of genomics, and offers suggestions for future research priorities in order to initiate the advance research on disease resistance. This book is immensely useful to the researchers especially Brassica breeders, teachers, extension specialists, students, industrialists, farmers, and all others who are interested to grow healthy, and profitable cruciferous crops all over the world.
Author: Heba I. Mohamed
Publisher: Springer Nature
ISBN: 3030665879
Category : Science
Languages : en
Pages : 672
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Book Description
Abiotic and biotic stress factors, including drought, salinity, waterlog, temperature extremes, mineral nutrients, heavy metals, plant diseases, nematodes, viruses, and diseases, adversely affect growth as well as yield of crop plants worldwide. Plant growth-promoting microorganisms (PGPM) are receiving increasing attention from agronomists and environmentalists as candidates to develop an effective, eco-friendly, and sustainable alternative to conventional agricultural (e.g., chemical fertilizers and pesticide) and remediation (e.g., chelators-enhanced phytoremediation) methods employed to deal with climate change-induced stresses. Recent studies have shown that plant growth-promoting bacteria (PGPB), rhizobia, arbuscular mycorrhizal fungi (AMF), cyanobacteria have great potentials in the management of various agricultural and environmental problems. This book provides current research of biofertilizers and the role of microorganisms in plant health, with specific emphasis on the mitigating strategies to combat plant stresses.
Author: Gobind Singh Saharan
Publisher: Springer
ISBN: 9811000212
Category : Science
Languages : en
Pages : 332
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Book Description
This book deals with the various aspects viz., the disease, geographical distribution, symptoms on different hosts, host range, yield losses, and disease assessment method, while detailed description on pathogen include taxonomic position, phylogeny, variability, sporulation, perpetuation, and spore germination, host-parasite interactions in the form of seed infection, disease cycle, process of infection, and pathogenesis, epidemiology, forecasting, fine structures, biochemical changes, and phytotoxins, host defence mechanism, techniques to study host-parasite relationships, and management practices including cultural, chemical, biological control practices, and deployment of host resistance. The last section deals with gaps in our understanding, and knowledge about management of these diseases, techniques for the diagnosis of disease and offer suggestions for future research priorities. Each aspect has been vividly illustrated with photographs, histograms, figures, tables, electron micrographs for stimulating, effective and easy reading and understanding. We are sure that this comprehensive encyclopaedic treatise on "Alternaria diseases of crucifers” will be of immense use to the researchers, teachers, students and all others who are interested in the diagnosis and management of Alternaria diseases of crucifers world over. Four species of Alternaria are wide spread and most devastating on cruciferous oil yielding crops, vegetables, forage crops, ornamental plants, and numerous weeds all over the world. The damage to the plants is in the form of infections on seed in the soil during sowing, on seedlings during emergence and growth, on stem, leaves, inflorescence, pods and finally to the seed causing yield losses in seed quantity and quality.
Author: Jameel M. Al-Khayri
Publisher: Springer Nature
ISBN: 3030736067
Category : Science
Languages : en
Pages : 595
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Book Description
This book provides up-to-date knowledge of the promising field of Nanobiotechnology with emphasis on the mitigation approaches to combat plant abiotic stress factors, including drought, salinity, waterlog, temperature extremes, mineral nutrients, and heavy metals. These factors adversely affect the growth as well as yield of crop plants worldwide, especially under the global climate change. Nanobiotechnology is viewed to revolutionize crop productivity in future. The chapters discuss the status and prospects of this cutting-edge technology toward understanding tolerance mechanisms, including signaling molecules and enzymes regulation in addition to the applications of Nanobiotechnology to combat individual abiotic stress factors.
