Advanced Chromatographic and Electromigration Methods in BioSciences PDF Download
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Author: I. Mikšík
Publisher: Elsevier
ISBN: 0080858686
Category : Science
Languages : en
Pages : 1149
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Book Description
This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.
Author: I. Mikšík
Publisher: Elsevier
ISBN: 0080858686
Category : Science
Languages : en
Pages : 1149
Get Book Here
Book Description
This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted.Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.
Author: Robert L. Wixom
Publisher: John Wiley & Sons
ISBN: 1118060296
Category : Science
Languages : en
Pages : 357
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Book Description
Leading researchers discuss the past and present of chromatography More than one hundred years after Mikhail Tswett pioneered adsorption chromatography, his separation technique has developed into an important branch of scientific study. Providing a full portrait of the discipline, Chromatography: A Science of Discovery bridges the gap between early, twentieth-century chromatography and the cutting edge of today’s research. Featuring contributions from more than fifty award-winning chromatographers, Chromatography offers a multifaceted look at the development and maturation of this field into its current state, as well as its importance across various scientific endeavors. The coverage includes: Consideration of chromatography as a unified science rather than just a separation method Key breakthroughs, revolutions, and paradigm shifts in chromatography Profiles of Nobel laureates who used chromatography in their research, and the role it played Recent advances in column technology Chromatography’s contributions to the agricultural, space, biological/medical sciences; pharmaceutical science; and environmental, natural products, and chemical analysis Future trends in chromatography With numerous references and an engaging series of voices, Chromatography: A Science of Discovery offers a diverse look at an essential area of science. It is a unique and invaluable resource for researchers, students, and other interested readers who seek a broader understanding of this field.
Author: Fred Smith
Publisher: Elsevier
ISBN: 0080472893
Category : Law
Languages : en
Pages : 585
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Book Description
The Handbook of Forensic Drug Analysis is a comprehensive chemical and analytic reference for the forensic analysis of illicit drugs. With chapters written by leading researchers in the field, the book provides in-depth, up-to-date methods and results of forensic drug analyses. This Handbook discusses various forms of the drug as well as the origin and nature of samples. It explains how to perform various tests, the use of best practices, and the analysis of results. Numerous forensic and chemical analytic techniques are covered including immunoassay, gas chromatography, and mass spectrometry. Topics range from the use of immunoassay technologies for drugs-of-abuse testing, to methods of forensic analysis for cannabis, hallucinogens, cocaine, opioids, and amphetamine. The book also looks at synthetic methods and law enforcement concerns regarding the manufacture of illicit drugs, with an emphasis on clandestine methamphetamine production. This Handbook should serve as a widely used reference for forensic scientists, toxicologists, pharmacologists, drug companies, and professionals working in toxicology testing labs, libraries, and poison control centers. It may also be used by chemists, physicians and those in legal and regulatory professions, and students of graduate courses in forensic science. - Contributed to by leading scientists from around the world - The only analysis book dedicated to illicit drugs of abuse - Comprehensive coverage of sampling methods and various forms of analysis
Author: Ibolya Molnár-Perl
Publisher: Elsevier
ISBN: 0080458459
Category : Science
Languages : en
Pages : 669
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Book Description
Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC).The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines.* Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes* Compares advantages/disadvantages of various chromatographic techniques* Readers can undertake practical tasks using detailed protocols given in the book
Author: A. Stoyanov
Publisher: Elsevier
ISBN: 0080518966
Category : Science
Languages : en
Pages : 411
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Book Description
The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here. Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.
Author: Jack Cazes
Publisher: CRC Press
ISBN: 1439870675
Category : Science
Languages : en
Pages : 2968
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Book Description
Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.
Author: Richard A Frazier
Publisher: Royal Society of Chemistry
ISBN: 1847550312
Category : Technology & Engineering
Languages : en
Pages : 143
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Book Description
Since its inception in the early 1980s, capillary electrophoresis (CE) offers a great deal of flexibility as a modern analytical technique, and has found applications within many fields of analysis, particularly pharmaceutical science and biochemistry. Until now, food analysts have had difficulties in adopting the technique due to the lack of written guidance. Capillary Electrophoresis for Food Analysis: Method Development provides basic information and the support needed to enable food analysts to utilise the technique for the development of new separation methods. Designed specifically for the needs of food analysts, the book takes the reader step by step through the process of developing and troubleshooting CE methods. Worked examples are included to make it ideal as a laboratory companion as well as a library reference source.
Author: Irina V. Perminova
Publisher: Springer Science & Business Media
ISBN: 9781402032509
Category : Science
Languages : en
Pages : 544
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Book Description
Effective remediation of polluted environments is a priority in both Eastern and Western countries. In the U.S. and Europe, remediation costs generally exceed the net economic value of the land. As a result, scientists and engineers on both sides of the Atlantic have aggressively tried to develop novel technologies to meet regulatory standards at a fraction of the costs. In situ remediation shows considerable promise from both technical and economic perspectives. In situ technologies that deploy natural attenuating agents such as humic substances (HS) may be even more cost effective. Numerous studies have shown humics capable of altering both the chemical and the physical speciation of the ecotoxicants and in turn attenuate potential adverse environmental repercussions. Furthermore, the reserves of inexpensive humic materials are immense. Which suggests HS portend great promise as inexpensive amendments to mitigate the environmental impacts of ecotoxicants and as active agents in remediation. To elucidate emerging concepts of humics-based remediation technologies, we organized the NATO Advanced Research Workshop (ARW), entitled "Use of humates to remediate polluted environments: from theory to practice", held on September 23-29, 2002 in Zvenigorod, Russia (see the web-site http://www.mgumus.chem.msu.ru/arw).
Author: Toshihiko Hanai
Publisher: Royal Society of Chemistry
ISBN: 1782621504
Category : Science
Languages : en
Pages : 352
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Book Description
The coupling of mass spectrometry or nuclear magnetic resonance to chromatography has broadened the possibilities for determining organic reaction mechanisms. And while many results have been published reporting these, even more can be achieved through modern computational methods. Combining computational and theoretical techniques with advanced chromatographic methods offers a powerful tool for quantitatively determining molecular interactions . This book presents the possibilities for characterising biological applications by combining analytical and computational chemistries. Written by the author of “HPLC: A Practical Guide” (RSC, 1999), the book examines not only the behaviour of biological reactions per se, but also describes the behaviour of biological molecules in chromatography systems. Various software packages are reviewed, and most computations can be performed on a standard PC using accessible software. Consideration is given to a variety of chromatographic techniques and strategies for high-sensitivity detection are presented. The first book of its kind, it will inspire readers to explore the possibilities of combining these techniques in their own work, whether at an industrial or academic level.
Author: Jiarui Xu
Publisher: Royal Society of Chemistry
ISBN: 1782621539
Category : Technology & Engineering
Languages : en
Pages : 352
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Book Description
Mechanochromic fluorescent (or mechanofluorochromic) materials change their emission colours (spectra) when an appropriate external mechanical force stimulus is applied. This is an important group of materials with a huge range of applications, including use in sensors, memory chips, security inks, and light devices. Mechanochromic Fluorescent Materials introduces the reader to the concept of mechanofluorochromism and the variety of applications of this group of materials. Prominent international figures in mechanofluorochromism consider the innovative research in this field over the last ten years. Chapters provide in depth coverage of most reported mechanofluorochromic systems, including organic and organic-inorganic complexes; polymer and polymer composites; and aggregation-induced emission. This book is aimed to inform all students and researchers with an interest in mechanofluorochromism, and to help researchers identify and synthesize more of these materials, and develop the study and application of mechanofluorochromic materials.
