Adaptive Immunity, Gene Silencing and Viral Silencing Suppressor Proteins Play a Role in the Bipartite Begomovirus-plant Interaction PDF Download
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Author: Charles Hagen
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Book Description
Author: Charles Hagen
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Book Description
Author: R.K. Gaur
Publisher: Academic Press
ISBN: 0128244836
Category : Science
Languages : en
Pages : 584
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Book Description
Plant Virus-Host Interaction: Molecular Approaches and Viral Evolution, Second Edition, provides comprehensive coverage of molecular approaches for virus-host interaction. The book contains cutting-edge research in plant molecular virology, including pathogenic viroids and transport by insect vectors, interference with transmission to control viruses, synergism with pivotal coverage of RNA silencing, and the counter-defensive strategies used by viruses to overcome the silencing response in plants. This new edition introduces new, emerging proteins involved in host-virus interactions and provides in-depth coverage of plant virus genes’ interactions with host, localization and expression. With contributions from leading experts, this is a comprehensive reference for plant virologists, molecular biologists and others interested in characterization of plant viruses and disease management. Introduces new, emerging proteins involved during the host-virus interaction and new virus strains that invade new crops through recombination, resorting and mutation Provides molecular approaches for virus-host interaction Highlights RNA silencing and counter-defensive strategies for disease management Discusses the socioeconomic implications of viral spread and mitigation techniques
Author: Yule Liu
Publisher: Frontiers Media SA
ISBN: 2889452697
Category :
Languages : en
Pages : 165
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Book Description
Plant viruses impose a serious threat on agriculture, which motivates extensive breeding efforts for viral resistant crops and inspires lasting interests on basic research to understand the mechanisms underlying plant immunity against viruses. Viruses are obligate intracellular parasites. Their genomes are usually small and only encode a few products that are essential to hijack host machinery for their nucleotide and protein biosynthesis, and that are necessary to suppress host immunity. Plants evolved multilayers of defense mechanisms to defeat viral infection. In this research topic, we gathered 13 papers covering recent advances in different aspects of plant immunity against viruses, including reviews on RNA silencing and R gene based immunity and their application, translational initiation factor mediated recessive resistance, genome editing based viral immunity, role of chloroplast in plant-virus interaction, and research articles providing new mechanistic insights on plant-virus interactions. We hope that this Research Topic helps readers to have a better understanding of the progresses that have been made recently in plant immunity against viruses. A deeper understanding of plant antiviral immunity will facilitate the development of innovative approaches for crop protections and improvements.
Author: Tatjana Kleinow
Publisher: Springer
ISBN: 3319254898
Category : Science
Languages : en
Pages : 192
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Book Description
Plant RNA– and DNA-viruses have small genomes and with this limited coding capacity exhibit a strong dependency on host cellular processes and factors to complete their viral life cycle. Various interactions of viral proteins or nucleic acids with host components (proteins, nucleic acids, carbohydrates, lipids and metabolites) evolved, which are essential for a successful systemic spread of viruses within the plant. For example, in plants, transport of endogenous macromolecules like proteins and nucleic acids occurs in a highly selective and regulated manner and viruses exploit these specifically controlled trafficking pathways. Research on plant virus movement is located at the interface of molecular plant virology and plant cell biology. The proposed book project aims to give an overview on the current state of this research and to highlight novel insights into the dynamic interplay between plant viruses and host cells. The book is intended for researchers in plant biology and virology and especially written for those who aim to understand cell biology of virus-plant interactions.
Author: Gad Loebenstein
Publisher: Springer Science & Business Media
ISBN: 1402037805
Category : Science
Languages : en
Pages : 547
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Book Description
This book is a first attempt to link well-known plant resistance phenomena with emerging concepts in molecular biology. Resistance phenomena such as the local lesion response, induced resistance, "green islands" and resistance in various crop plants are linked with new information on gene-silencing mechanisms, gene silencing suppressors, movement proteins and plasmodesmatal gating, downstream signalling components, and more.
Author: Sangeeta Saxena
Publisher: Springer
ISBN: 9811059845
Category : Science
Languages : en
Pages : 332
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Book Description
Begomoviruses are one of the most interesting plant viruses to study for basic and applied research as they cause huge economic losses to agriculture industries and farmers all over the world. They belong to family Geminiviridae and are emergent plant viral pathogens which cause diseases in various crops in the tropical and subtropical regions. They are transmitted by the whitefly (B. tabaci) and have either one (monopartite DNA-A) or two (bipartite DNA-A and DNA-B) genomic components. DNA-A and DNA-B are of ~2600 - 2800 nucleotides each. A number of serious diseases of cultivated crops of the Fabaceae, Malvaceae, Solanaceae and Cucurbitaceae families are caused by Begomoviruses which are considered as threat to their cultivation in many countries. Accurate diagnosis is important for successful diseases management, since plants infected by Begomovirus do not recover, suffer serious yield losses and act as further sources of inoculum, which is then picked up and spread by their vector whitefly (B. tabaci). Reports of occurrence of new viruses and re-emergence of several known viruses in new niches have become regular event. In such a dynamic system, growth of several crop species relies on an accurate diagnosis, management and better understanding of the biology of the casual virus. This is crucial to evolve appropriate control practices and to prevent the virus infection. Researchers have achieved considerable progress in characterization, detection and management of virus on different crop species in the last decade. This book covers latest information in diagnosis of begomoviruses in the present scenario and explores the new vistas in the field of genomics and proteomics. Chapters in Section 1 illustrates the occurrence, genome organisation, transmission and diagnostics of begomoviruses. It also details the diseases caused by begomoviruses on different crops, detection techniques and management strategies in support of research findings by presentation of data, graphics, figures and tables. Section 2 is a chapterwise collection of occurrence, diversity and status of begomoviruses in Asian Africa counties where the diseases are most prevalent. This book will provide wide opportunity to the readers to have complete information and status of begomovirus in Asia and Africa. This will be useful resource for researchers and extension workers involved in the begomvirus disease diagnosis and molecular biology. Expert detection, accurate diagnosis and timely management play a significant role in keeping plants free from pathogens. In this book expert researchers share their research knowledge and literature which are vital towards the diagnosis of begomoviruses, addressing traditional plant pathology techniques as well as advanced molecular diagnostic approach. The book deals with the economically important crops including fruits, vegetables along with challenges in crop protection against diseases caused by begomovirus. This will be resourceful and handy for researcher, practitioners and also students.
Author:
Publisher: Academic Press
ISBN: 0080923089
Category : Science
Languages : en
Pages : 280
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Book Description
Viruses are a huge threat to agriculture. In the past, viruses used to be controlled using conventional methods, such as crop rotation and destruction of the infected plants, but now there are more novel ways to control them. This volume focuses on topics that must be better understood in order to foster future developments in basic and applied plant virology. These range from virus epidemiology and virus/host co-evolution and the control of vector-mediated transmission through to systems biology investigations of virus-cell interactions. Other chapters cover the current status of signalling in natural resistance and the potential for a revival in the use of cross-protection, as well as future opportunities for the deployment of the under-utilized but highly effective crop protection strategy of pathogen-derived resistance. Contributions from leading authorities Informs and updates on all the latest developments in the field
Author: John M. Watson
Publisher: Humana Press
ISBN: 9781617798818
Category : Science
Languages : en
Pages : 380
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Book Description
Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.
Author: Guido Sessa
Publisher: John Wiley & Sons
ISBN: 0470959509
Category : Science
Languages : en
Pages : 312
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Book Description
Molecular Plant Immunity provides an integrated look at both well-established and emerging concepts in plant disease resistance providing the most current information on this important vitally important topic within plant biology. Understanding the molecular basis of the plant immune system has implications on the development of new varieties of sustainable crops, understanding the challenges plant life will face in changing environments, as well as providing a window into immune function that could have translational appeal to human medicine. Molecular Plant Immunity opens with chapters reviewing how the first line of plant immune response is activated followed by chapters looking at the molecular mechanisms that allow fungi, bacteria, and oomycetes to circumvent those defenses. Plant resistance proteins, which provide the second line of plant immune defense, are then covered followed by chapters on the role of hormones in immunity and the mechanisms that modulate specific interaction between plants and viruses. The final chapters look at model plant-pathogen systems to review interaction between plants and fungal, bacterial, and viral pathogens. Written by a leading team of international experts, Molecular Plant Immunity will provide a needed resource to diverse research community investigated plant immunity.
Author: Tomas Adan Melgarejo Gutierrez
Publisher:
ISBN: 9781321363395
Category :
Languages : en
Pages :
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Book Description
Begomoviruses are an emergent and economically important group of single stranded DNA viruses in tropical and subtropical regions worldwide. In the second chapter the biological and molecular properties of three strains of the bipartite begomovirus Jatropha mosaic virus (JMV) from the Dominican Republic (DO) were determined. Jatropha plants inoculated with the infectious clones of these JMV strains developed yellow mosaic symptoms, which fulfilled Koch's postulates. For this disease. Furthermore, these JMV strains also infected the crop plants, tobacco and common bean, revealing a possible link between weed- and crop-infecting begomoviruses. Finally, asymmetry in replication and infectivity of pseudorecombinants formed between the DNA components of these strains was consistent with a high degree of genetic diversity,and suggested a long history of local evolution of JMV in the DO. In the third chapter, the first bona fide New World monopartite begomovirus Tomato leaf deformation virus (ToLDeV) was characterized, and shown to be the causal agent of tomato leaf curl disease in Peru and Ecuador based on multimeric clones of the genomic DNA of three ToLDeV genotypes, delivered via agroinoculation, inducing leaf curl symptoms in tomato indistinguishable from those observed in the tomato fields. Biological properties of ToLDeV were similar to those of Old World monopartite tomato-infecting begomoviruses, including lack of sap-transmissibility, phloem limitation, a resistance phenotype in tomato germplasm with the Ty-1 gene and functional properties of the V1 (capsid protein) and C4 genes. Differences in symptom phenotypes induced by the ToLDeV genotypes in tomato (e.g., whether a recovery phenotype was observed or not) and N. benthamiana plants were associated with a highly divergent left intergenic region and C4 gene. Finally, evidence is presented that the ToLDeV genotypes emerged from the DNA-A component of a New World bipartite progenitor via parallel evolution, mutation and recombination. In the fourth chapter, the functions of the ToLDeV C2 (TrAP) and C4 genes were investigated by mutational analysis and ectopic expression via two viral vectors with distinct tissue tropism (phloem-limited and non-phloem limited). ToLDeV C2 mutants replicated in N. benthamiana leaf discs, but the infectivity was host-specific. The capacity of the TrAP and C4 viral proteins to suppress gene silencing was evaluated. Neither TrAP nor C4 protein suppressed local silencing; however, C4 acted as a strong suppressor of long-distance silencing. Both C2 and C4 showed some capacity to suppress transcriptional gene silencing based on restoring GFP expression in transcriptionally silenced 16c-TGS plants. Moreover, a high level of cytosine methylation in the left intergenic region of ToLDeV was associated with tomato plants showing the recovery phenotype following infection with ToLDeV. Finally, subcellular localization studies revealed that the TrAP protein localized to the nucleus, whereas C4 was localized to the plasma membrane.
