A Study of the Mechanism of Retention in Reversed-phase Liquid Chromatography PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download A Study of the Mechanism of Retention in Reversed-phase Liquid Chromatography PDF full book. Access full book title A Study of the Mechanism of Retention in Reversed-phase Liquid Chromatography by Thomas Gerard Burke. Download full books in PDF and EPUB format.
Author: Thomas Gerard Burke
Publisher:
ISBN:
Category :
Languages : en
Pages : 98
Get Book Here
Book Description
Author: Thomas Gerard Burke
Publisher:
ISBN:
Category :
Languages : en
Pages : 98
Get Book Here
Book Description
Author: Lay Choo Tan
Publisher:
ISBN:
Category :
Languages : en
Pages : 628
Get Book Here
Book Description
Author: Lynn Alison Cole
Publisher:
ISBN:
Category :
Languages : en
Pages : 338
Get Book Here
Book Description
Author: Won Jo Cheong
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 690
Get Book Here
Book Description
Author: Azhar Salman Alhejji
Publisher:
ISBN: 9781303127410
Category :
Languages : en
Pages : 88
Get Book Here
Book Description
Author: M.C. García-Álvarez-Coque
Publisher: Elsevier Inc. Chapters
ISBN: 0128071311
Category : Science
Languages : en
Pages : 29
Get Book Here
Book Description
The addition of reagents to an RPLC mobile phase enables the separation of ionizable compounds, inorganic anions, and metal ions using conventional instrumentation, silica-based materials, and hydro-organic mixtures, thanks to a variety of secondary equilibria. This gives rise to several chromatographic modes, whose main features are outlined in this chapter. The effect of the mobile phase pH on the retention of ionizable compounds is described, together with the recommended experimental practice. The mechanism of adsorption of amphiphilic anions or cations on the stationary phase to attract analytes with opposite charge or suppress the silanol activity is discussed. Different reagents, such as alkylammonium salts, surfactants (below and above the critical micelle concentration), perfluorinated carboxylate anions, chaotropic ions, and ionic liquids, are considered. The potential of metal chelation for the determination of metal ions and organic compounds is also summarized.
Author: Jun Dai
Publisher:
ISBN:
Category :
Languages : en
Pages : 626
Get Book Here
Book Description
Author: Sophie Bailes
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Michael W. Dong
Publisher: John Wiley & Sons
ISBN: 1119313767
Category : Science
Languages : en
Pages : 410
Get Book Here
Book Description
A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.
Author: Tarun S. Patel
Publisher:
ISBN:
Category : Adsorption
Languages : en
Pages : 272
Get Book Here
Book Description
