A Practical Guide to HPLC Detection PDF Download
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Author: Donald Parriott
Publisher: Academic Press
ISBN: 0323140610
Category : Science
Languages : en
Pages : 304
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Book Description
This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems. - Addresses the practical aspects of HPLC detection, including: basic theory, when a particular type of detector can be used, how detectors from various manufacturers differ, common problems of detectors and ways to avoid them - Presents an overview of today's most common techniques - Discusses the advantages and disadvantages of HPLC, dispelling common misconceptions
Author: Donald Parriott
Publisher: Academic Press
ISBN: 0323140610
Category : Science
Languages : en
Pages : 304
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Book Description
This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems. - Addresses the practical aspects of HPLC detection, including: basic theory, when a particular type of detector can be used, how detectors from various manufacturers differ, common problems of detectors and ways to avoid them - Presents an overview of today's most common techniques - Discusses the advantages and disadvantages of HPLC, dispelling common misconceptions
Author: Toshihiko Hanai
Publisher: Royal Society of Chemistry
ISBN: 9780854045150
Category : Science
Languages : en
Pages : 152
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Book Description
How can these compounds be separated? Why was that method used? These are the two basic questions often asked by students of chromatography. HPLC: A Practical Guide provides the answers, enabling the reader to grasp the concepts of the technique using simple, representative chromatograms. Divided into six chapters, this practical guide covers basic concepts of HPLC; instrumentation; stationary phase materials; eluents; column efficiency; and the influence of physical chemistry on separations. Focusing on the basic considerations such as selection of stationary phase and eluent, rather than specific applications, sections on troubleshooting are also included. Uniquely, the descriptions of chromatographic separations are based on solubility using molecular properties, and solubility parameters are used to analyse the selections of chromatographic mode and column. Presenting the chemistry of liquid chromatography for undergraduate students, this valuable practical guide will also be useful for laboratory staff in industry and academia.
Author: Lloyd R. Snyder
Publisher: John Wiley & Sons
ISBN: 1118591518
Category : Science
Languages : en
Pages : 665
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Book Description
This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
Author: Serban C. Moldoveanu
Publisher: Elsevier
ISBN: 0128037113
Category : Science
Languages : en
Pages : 600
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Book Description
Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation. - Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results - Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field - Provides criteria for HPLC method selection, development, and validation - Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field
Author: Toshihiko T Hanai
Publisher: Royal Society of Chemistry
ISBN: 1847551076
Category : Science
Languages : en
Pages : 146
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Book Description
How can these compounds be separated? Why was that method used? These are the two basic questions often asked by students of chromatography. HPLC: A Practical Guide provides the answers, enabling the reader to grasp the concepts of the technique using simple, representative chromatograms. Divided into six chapters, this practical guide covers basic concepts of HPLC; instrumentation; stationary phase materials; eluents; column efficiency; and the influence of physical chemistry on separations. Focusing on the basic considerations such as selection of stationary phase and eluent, rather than specific applications, sections on troubleshooting are also included. Uniquely, the descriptions of chromatographic separations are based on solubility using molecular properties, and solubility parameters are used to analyse the selections of chromatographic mode and column. Presenting the chemistry of liquid chromatography for undergraduate students, this valuable practical guide will also be useful for laboratory staff in industry and academia.
Author: Erno Pungor
Publisher: CRC Press
ISBN: 1000099423
Category : Science
Languages : en
Pages : 395
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Book Description
A Practical Guide to Instrumental Analysis covers basic methods of instrumental analysis, including electroanalytical techniques, optical techniques, atomic spectroscopy, X-ray diffraction, thermoanalytical techniques, separation techniques, and flow analytical techniques. Each chapter provides a brief theoretical introduction followed by basic and special application experiments. This book is ideal for readers who need a knowledge of special techniques in order to use instrumental methods to conduct their own analytical tasks.
Author: Donald A Wellings
Publisher: Elsevier
ISBN: 0080458858
Category : Technology & Engineering
Languages : en
Pages : 193
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Book Description
This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...•Provides practical, hands-on advice based on years of experience•Will help ensure optimal design, equipment and separation results for your particular task•Presents system layouts from laboratory to process scale•Will help you to devise or improve record-keeping and documentation systems·Provides practical, hands-on advice based on years of experience·Will help ensure optimal design, equipment and separation results for your particular task·Presents system layouts from laboratory to process scale·Will help you to devise or improve record-keeping and documentation systems
Author: Joel K. Swadesh
Publisher: CRC Press
ISBN: 9780849326820
Category : Medical
Languages : en
Pages : 396
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Book Description
High performance liquid chromatography (HPLC) is a technique used in diverse laboratory and industrial settings for the separation of components of complex mixtures. HPLC: Practical and Industrial Applications is a trouble-shooting, problem-solving guide for scientists, engineers, and technicians who use HPLC in their day-to-day work. It provides the answers to specific problems and includes practical case studies. This case history approach to chromatography is an effective teaching tool and clearly illustrates how to use techniques such as reversed phase chromatography, ion exchange chromatography, gel permeation chromatography, and capillary electrophoresis. The book is organized to facilitate rapid understanding for those working with a particular area of chromatography: Introduces the reader to instrumentation, reviews basic chromatographic theory, and presents a brief survey of absorbance fluorescence and refractive index detectors. Provides a broad view of the role of the analytical laboratory in an industrial organization. Offers suggestions on optimizing the utilization of personnel and work flow in the laboratory. Covers process sampling and analysis · Describes process chromatography. The remaining chapters discuss specialties within separations technology, including an outline of the key features of each technique, a thorough bibliography, a list of precedents, and detailed examples of one or more applications presented from the viewpoint of industrial and basic scientists. Specialty detectors are also described. HPLC: Practical and Industrial Applications is an essential reference for those working in the industrial sector, as well as scientists, students, technicians interested in learning HPLC methods.
Author: Oona McPolin
Publisher: Lulu.com
ISBN: 0956152805
Category : Medical
Languages : en
Pages : 150
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Book Description
If you are new to HPLC, this book provides an invaluable guide to how HPLC is actually used when analysing pharmaceuticals. It is full of practical advice on the operation of HPLC systems combined with the necessary theoretical knowledge to ensure understanding of the technique. Key features include: A thorough discussion of the stationary phase enabling the reader to make sense of the many parameters used to describe a HPLC column; Practical advice and helpful hints for the preparation and use of mobile phase; A complete overview of each of the different components which together make up a HPLC system; A description of the contents of a typical HPLC analytical method and how to interpret these; A step-by-step guide on how to follow a method and set up a HPLC analysis; A discussion of system suitability criteria and how to interpret the values obtained during an analysis; Explanation of the common methods of calibration and quantification used for pharmaceutical analysis.
Author: William W. Christie
Publisher: Pergamon
ISBN:
Category : Science
Languages : en
Pages : 296
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Book Description
A comprehensive guide to lipids and lipid qnalysis using high-performance liquid chromatography. The author covers the construction and occurrence of various lipids and their functions. The book introduces the values of high-performance liquid chromatography, its theoretical considerations and the use of equipment, and discusses the separation of both simple and complex lipid classes. In combining the theoretical and practical sides of lipid anlaysis this book will be of immense value to all those involved in lipid research.
