A Gene Expression System Based on Bacteriophage RNA Polymerases and Characterization of Bacteriophage T7 RNA Polymerase PDF Download
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Author: Biao He
Publisher:
ISBN:
Category :
Languages : en
Pages : 438
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Author: Biao He
Publisher:
ISBN:
Category :
Languages : en
Pages : 438
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Author: Lyndon B. Gross
Publisher:
ISBN:
Category :
Languages : en
Pages : 184
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
Author: Claire Ellen Morris
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 410
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Author: Joseph M. Fernandez
Publisher: Elsevier
ISBN: 0080532357
Category : Science
Languages : en
Pages : 493
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Book Description
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. - Provides detailed information on expression systems - Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs - Includes timelines and examples - Compares pros and cons of each method
Author: John F. Klement
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 410
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Author: Howard Colgate Towle
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 384
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.
Author: Eugene Thaddeus Butler
Publisher:
ISBN:
Category :
Languages : en
Pages : 464
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Author: Alan Magee
Publisher:
ISBN:
Category :
Languages : en
Pages :
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