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Author: Zuzana Koledova
Publisher: Humana Press
ISBN: 9781493970193
Category : Science
Languages : en
Pages : 452
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Book Description
This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.
Author: Beate Brand-Saberi
Publisher: Springer Nature
ISBN: 3030339238
Category : Medical
Languages : en
Pages : 247
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Book Description
This textbook describes the biology of different adult stem cell types and outlines the current level of knowledge in the field. It clearly explains the basics of hematopoietic, mesenchymal and cord blood stem cells and also covers induced pluripotent stem cells. Further, it includes a chapter on ethical aspects of human stem cell research, which promotes critical thinking and responsible handling of the material. Based on the international masters program Molecular and Developmental Stem Cell Biology taught at Ruhr-University Bochum and Tongji University Shanghai, the book is a valuable source for postdocs and researchers working with stems cells and also offers essential insights for physicians and dentists wishing to expand their knowledge. This textbook is a valuable complement to Concepts and Applications of Stem Cell Biology, also published in the Learning Materials in Biosciences textbook series.
Author: Zuzana Koledova
Publisher: Humana Press
ISBN: 9781493970193
Category : Science
Languages : en
Pages : 452
Get Book Here
Book Description
This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.
Author: John Haycock
Publisher: Humana Press
ISBN: 9781607619833
Category : Science
Languages : en
Pages : 0
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Book Description
Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.
Author: Gary S. Stein
Publisher: John Wiley & Sons
ISBN: 0470889896
Category : Science
Languages : en
Pages : 439
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Book Description
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.
Author: Christine L. Mummery
Publisher: Academic Press
ISBN: 0124115675
Category : Science
Languages : en
Pages : 446
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Book Description
The second edition of Stem Cells: Scientific Facts and Fiction provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. Building on success of its award-winning preceding edition, the second edition features new chapters on embryonic and iPS cells and stem cells in veterinary science and medicine. It contains major revisions on cancer stem cells to include new culture models, additional interviews with leaders in progenitor cells, engineered eye tissue, and xeno organs from stem cells, as well as new information on "organs on chips" and adult progenitor cells. In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues that everyone presumed were unable to regenerate in adults, the heart and the brain in particular. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson's, diabetes, and spinal cord lesions, based on the use of stem cells; and from entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. - Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy - Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice - Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice
Author: Cornelia Kasper
Publisher: Springer Nature
ISBN: 3030667499
Category : Medical
Languages : en
Pages : 252
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Book Description
This textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, in vitro test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of the essential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.
Author:
Publisher:
ISBN: 9780470151808
Category : Stem cells
Languages : en
Pages :
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Book Description
Author: Scott Camazine
Publisher: Princeton University Press
ISBN: 0691212929
Category : Science
Languages : en
Pages : 548
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Book Description
The synchronized flashing of fireflies at night. The spiraling patterns of an aggregating slime mold. The anastomosing network of army-ant trails. The coordinated movements of a school of fish. Researchers are finding in such patterns--phenomena that have fascinated naturalists for centuries--a fertile new approach to understanding biological systems: the study of self-organization. This book, a primer on self-organization in biological systems for students and other enthusiasts, introduces readers to the basic concepts and tools for studying self-organization and then examines numerous examples of self-organization in the natural world. Self-organization refers to diverse pattern formation processes in the physical and biological world, from sand grains assembling into rippled dunes to cells combining to create highly structured tissues to individual insects working to create sophisticated societies. What these diverse systems hold in common is the proximate means by which they acquire order and structure. In self-organizing systems, pattern at the global level emerges solely from interactions among lower-level components. Remarkably, even very complex structures result from the iteration of surprisingly simple behaviors performed by individuals relying on only local information. This striking conclusion suggests important lines of inquiry: To what degree is environmental rather than individual complexity responsible for group complexity? To what extent have widely differing organisms adopted similar, convergent strategies of pattern formation? How, specifically, has natural selection determined the rules governing interactions within biological systems? Broad in scope, thorough yet accessible, this book is a self-contained introduction to self-organization and complexity in biology--a field of study at the forefront of life sciences research.
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309676681
Category : Medical
Languages : en
Pages : 155
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Book Description
Because of the recent advances in embryo modeling techniques, and at the request of the Office of Science Policy in the Office of the Director at the National Institutes of Health, the National Academies of Sciences, Engineering, hosted a 1-day public workshop that would explore the state of the science of mammalian embryo model systems. The workshop, which took place on January 17, 2020, featured a combination of presentations, panels, and general discussions, during which panelists and participants offered a broad range of perspectives. Participants considered whether embryo model systems - especially those that use nonhuman primate cells - can be used to predict the function of systems made with human cells. Presentations provided an overview of the current state of the science of in vitro development of human trophoblast. This publication summarizes the presentation and discussion of the workshop.
Author:
Publisher: Academic Press
ISBN: 9780128134818
Category : Science
Languages : en
Pages : 150
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Book Description
Advances in Stem Cells and Their Niches addresses stem cells during development, homeostasis, and disease/injury of the respective organs, presenting new developments in the field, including new data on disease and clinical applications. Video content illustrates such areas as protocols, transplantation techniques, and work with mice. Explores not only reviews of research, but also shares methods, protocols, and transplantation techniques Contains video content to illustrate such areas as protocols, transplantation techniques, and work with mice Each volume concentrates on one organ, making this a unique publication
