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Author: SERGEI. LEBEDEV
Publisher:
ISBN: 9781800249240
Category :
Languages : en
Pages :
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Book Description
Author: SERGEI. LEBEDEV
Publisher:
ISBN: 9781800249240
Category :
Languages : en
Pages :
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Book Description
Author: Herbert George Wells
Publisher:
ISBN:
Category : Comets
Languages : en
Pages : 394
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Book Description
Author: John Christopher
Publisher:
ISBN:
Category : Business enterprises
Languages : en
Pages : 280
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Book Description
Author: Amaya Azqueta
Publisher: Frontiers Media SA
ISBN: 2889196496
Category : Electrophoresis
Languages : en
Pages : 176
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Book Description
By means of this ‘Frontiers in Genetics’ research topic, we are celebrating 30 years of the Comet Assay. The first paper on this single-cell gel electrophoresis assay was published in 1984 by O. Ostling and K.J. Johanson (Biochem. Biophys. Res. Commun. Vol.123: 291-298). The comet assay is a versatile and sensitive method for measuring single - and double-strand breaks in DNA. By including lesion-specific enzymes in the assay, its range and sensitivity are greatly increased, but it is important to bear in mind that their specificity is not absolute. The comet assay (with and without inclusion of lesion-specific enzymes) is widely used as a biomarker assay in human population studies - primarily to measure DNA damage, but increasingly also to assess the capacity of cells for DNA repair. Ostling and Johanson (Biochem. Biophys. Res. Commun., 1984) were also the first to report experiments to measure DNA repair, by simply following the decrease of DNA damage over time after challenging cells with ionising radiation. However, this approach is time-consuming and laborious as it requires an extended period of cell culture and is therefore not ideal for biomonitoring studies, which typically require high-throughput processing of many samples. As an alternative approach, the in vitro comet-based repair assay was developed: a cell extract is incubated with a DNA substrate containing specific lesions, and DNA incisions accumulate. The in vitro comet-based repair assay has been modified and improved over the past decade: it was first devised to measure base excision repair of oxidised purines in lymphocytes (Collins et al., Mutagenesis, 2001), but has since been adapted for other lesions and thus other repair pathways, as well as being applied to tissue samples in addition to cell suspensions. Even after 30 years, the comet assay is still in a growth phase, with many new users each year. Many questions are repeatedly raised, which may seem to have self-evident answers, but clearly, it is necessary to reiterate them for the benefit of the new audience, and sometimes being forced to think again about old topics can shed new light. Different applications of the comet assay are discussed in this special issue, including: genotoxicity testing in different organisms, human biomonitoring, DNA repair studies, environmental biomonitoring and clinical studies. Furthermore, we consider and where possible answer questions, including the ones raised by Raymond Tice at the 8th International Comet Assay Workshop in Perugia (Italy 2009): What is the spectrum of DNA damage detected by the various versions of the comet assay?; What are the limitations associated with each application?; What should be done to standardize the assay for biomonitoring studies?; Can the comet assay be used to monitor changes in global methylation status?; What cell types are suitable for detecting genotoxic substances and their effects in vivo and in vitro?; Can the assay be fully automated?; and more. So this ‘Frontiers in Genetics’ research topic is written for the beginner as well as for the experienced users of the comet assay.
Author: George Bishop (Jr.)
Publisher:
ISBN: 0345516001
Category : Adolescence
Languages : en
Pages : 338
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Book Description
Fourteen-year-old Alan Broussard is swept up in his science teacher father's community-wide comet-watching activities, which illuminate for the young teen his father's inadequacies, his mother's unhappiness, and his own loss of innocence.
Author: Dom Testa
Publisher: Tor Teen
ISBN: 1429981601
Category : Young Adult Fiction
Languages : en
Pages : 244
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Book Description
In the gripping start to this young adult science fiction adventure series by popular Colorado radio host Dom Testa, the teenage crew of the starship Galahad must find a new home for humanity among the stars--if they fail, it will be the end of the human race.... When the tail of the comet Bhaktul flicks through the Earth's atmosphere, deadly particles are left in its wake. Suddenly, mankind is confronted with a virus that devastates the adult population. Only those under the age of eighteen seem to be immune. Desperate to save humanity, a renowned scientist proposes a bold plan: to create a ship that will carry a crew of 251 teenagers to a home in a distant solar system. Two years later, the Galahad and its crew—none over the age of sixteen—is launched. Two years of training have prepared the crew for the challenges of space travel. But soon after departing Earth, they discover that a saboteur is hiding on the Galahad! Faced with escalating acts of vandalism and terrorized by threatening messages, sixteen-year-old Triana Martell and her council soon realize that the stowaway will do anything to ensure that the Galahad never reaches its destination. The teens must find a way to neutralize their enemy. For if their mission fails, it will mean the end of the human race.... At the Publisher's request, this title is being sold without Digital Rights Management Software (DRM) applied.
Author: François Arago
Publisher:
ISBN:
Category : Comets
Languages : en
Pages : 40
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Book Description
Author: Gregory Benford
Publisher:
ISBN: 9780984915460
Category : Fiction
Languages : en
Pages : 513
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Book Description
A group of men and women, from a shattered society, ride a ball of ice through the solar system in search of a future.
Author: Joseph Whitaker
Publisher:
ISBN:
Category : Almanacs, English
Languages : en
Pages : 1082
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Book Description
Author: W. E. B. Du Bois
Publisher: Graphic Arts Books
ISBN: 1513298348
Category : Fiction
Languages : en
Pages : 19
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Book Description
The Comet (1920) is a science fiction story by W. E. B. Du Bois. Written while the author was using his role at The Crisis, the official magazine of the NAACP, to publish emerging black artists of the Harlem Renaissance, The Comet is a pioneering work of speculative fiction which imagines a catastrophic event not only decimating New York City, but bringing an abrupt end to white supremacy. “How silent the street was! Not a soul was stirring, and yet it was high-noon—Wall Street? Broadway? He glanced almost wildly up and down, then across the street, and as he looked, a sickening horror froze in his limbs.” Sent to the vault to retrieve some old records, bank messenger Jim Davis emerges to find a city descended into chaos. A comet has passed overhead, spewing toxic fumes into the atmosphere. All of lower Manhattan seems frozen in time. It takes him a few moments to see the bodies, piled into doorways and strewn about the eerily quiet streets. When he comes to his senses, he finds a wealthy woman asking for help. Soon, it becomes clear that they could very well be the last living people in the planet, that the fate of civilization depends on their ability to come together, not as black and white, but as two human beings. But how far will this acknowledgment take them? With a beautifully designed cover and professionally typeset manuscript, this edition of W. E. B. Du Bois’ The Comet is a classic work of African American literature reimagined for modern readers.
