Author: John Warren Williams
Publisher:
ISBN:
Category : Macromolecules
Languages : en
Pages : 144

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Book Description

Author: Todd M. Schuster
Publisher: Springer Science & Business Media
ISBN: 1468468286
Category : Science
Languages : en
Pages : 359

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Book Description
There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.

Author: Peter Schuck
Publisher: CRC Press
ISBN: 1498768954
Category : Medical
Languages : en
Pages : 267

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Book Description
A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles. The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems. Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx

Author: David Scott
Publisher: Royal Society of Chemistry
ISBN: 1847552617
Category : Science
Languages : en
Pages : 613

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Book Description
Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

Author: Peter Schuck
Publisher: CRC Press
ISBN: 1498751164
Category : Medical
Languages : en
Pages : 318

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Book Description
Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to mast

Author: Stephen E. Harding
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 656

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Book Description
This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.

Author: Anthony R. Cooper
Publisher: Springer Science & Business Media
ISBN: 1461331625
Category : Technology & Engineering
Languages : en
Pages : 691

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Book Description
This book is a record of a symposium, "Ultrafiltration Membranes and Applications," which was held at the l78th National Meeting of the American Chemical Society in Washington, D.C., September 11-13, 1979. In organlzlng these sessions, I hoped to provide a comprehensive survey of the current state of ultrafiltration theory, the most recent advances in membrane technology, and a thorough treatment of existing applications and future directions for ultrafiltration. For me, the symposium was an outstanding success. It was a truly international forum with stimulating presentations and an enthusiastic audience. I hope that some of this spirit has spilled over into this volume, which is intended to reach a much wider audience. I am indebted to the Division of Colloid and Surface Chemistry of the American Chemical Society for their sponsorship. ANTHONY R. COOPER Palo Alto, California }larch, 1980 vii CONTENTS PART I. FUNDMfENTALS Fifteen Years of Ultrafiltration: Problems and Future Promises of an Adolescent Technology . . 1 Alan S. Michaels Production, Specification, and Some Transport Characteristics of Cellulose Acetate Ultrafil tration Membranes for Aqueous Feed Solutions 21 S. Sourirajan, Takeshi Matsuura Fu-Hung Hsieh and Gary R. Gildert Chemical and Morphological Effects of Solute Diffusion Through Block Copolymer Membranes 45 Yatin B. Thakore, Dien-Feng Shieh and Donald J. Lyman Practical Aspects in the Development of a Polymer Matrix for Ultrafiltration. 57 Israel Cabasso Permeability Parameters of a Novel Polyamide Membrane. ... - ...

Author: L.A. Osterman
Publisher: Springer
ISBN: 9783540127352
Category : Science
Languages : en
Pages : 368

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Book Description
Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.

Author: Gordon G. Hammes
Publisher: John Wiley & Sons
ISBN: 1118858913
Category : Science
Languages : en
Pages : 504

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Book Description
This book provides an introduction to physical chemistry that is directed toward applications to the biological sciences. Advanced mathematics is not required. This book can be used for either a one semester or two semester course, and as a reference volume by students and faculty in the biological sciences.

Author: Susumu Uchiyama
Publisher: Springer
ISBN: 443155985X
Category : Science
Languages : en
Pages : 520

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Book Description
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.