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Author: Rony Seger
Publisher: Springer Science & Business Media
ISBN: 1592596711
Category : Science
Languages : en
Pages : 335
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Book Description
Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.
Author: Rony Seger
Publisher: Springer Science & Business Media
ISBN: 1592596711
Category : Science
Languages : en
Pages : 335
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Book Description
Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.
Author: Ethan Paritzky
Publisher: Trafford Publishing
ISBN: 1426917503
Category :
Languages : en
Pages : 251
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Book Description
Jake Meyer, a struggling attorney/private investigator and former special agent with the United States Treasury Department, is haunted by personal and professional tragedy. When he reluctantly returns to Zurich, Switzerland, where his demons were born, he begins a search for a long lost Pact, a secret land purchase between the Saudis and the Israelis, with help from Britain and the United States that could further enflame chaos in the Middle East and complicate the War on Terror. Standing in his way is a former Stasi operative, turned mercenary, who is hired by a cabal of multi-national corporations with a sinister interest in the secret agreement. His job is to find the Pact or to eliminate Meyer and any opposition. But Jake is aided by a highly experienced female Mossad agent who has recently returned to work after suffering her own loss. She is to protect and assist Jake at all costs as a matter of national security. Along the way Jake will suffer self-doubt and betrayals that impede his ability to find the Pact and exorcise his past demons. Yet, to save himself and the world from chaos and disaster, Jake will have to break through the walls and regain a measure of trust in other human beings.
Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 0896034593
Category : Science
Languages : en
Pages : 997
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Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyTM series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field.
Author: Itzhak Galnoor
Publisher: State University of New York Press
ISBN: 1438403720
Category : History
Languages : en
Pages : 399
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Book Description
Some seventy-five years after the boundaries of the British Mandate for Palestine were set, the State of Israel still lacks a defined territory and agreed-upon boundaries, except for its boundary with Egypt. This book examines this unusual situation, concentrating especially on the perceptions of territory and boundaries within the Zionist movement. Galnoor discusses the period from the first territorial decision in 1919 up to the establishment of the State of Israel in 1948, placing special emphasis on the relatively unknown Zionist, Palestinian, and Arab positions regarding territorial partition in 1937. And he argues that although dramatic changes have occurred in the international and regional arena, the partners to the conflict, the security considerations, and the international dilemmas, the 1937 decision contained the parameters of the choices that have confronted Arab and Israeli leaders ever since. His findings are of direct relevance to the ongoing Arab-Israeli peace negotiations, which once again revolve around the trade-off between national goals and territorial aspirations.
Author: Aaron Berman
Publisher: Wayne State University Press
ISBN: 0814344038
Category : Social Science
Languages : en
Pages : 231
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Book Description
A sophisticated analysis of how the Zionist understanding of the Holocaust shaped the development of American Jewish policies and political activism. Aaron Berman takes a moderate and measured approach to one of the most emotional issues in American Jewish historiography, namely, the response of American Jews to Nazism and the extermination of European Jewry.In remarkably large numbers, American Jews joined the Zionist crusade to create a Jewish state that would finally end the problem of Jewish homelessness, which they believed was the basic cause not only of the Holocaust but of all anti-Semitism. Though American Zionists could justly claim credit for the successful establishment of Israel in 1948, this triumph was not without cost. Their insistence on including a demand for Jewish statehood in any proposal to aid European Jewry politicized the rescue issue and made it impossible to appeal for American aid on purely humanitarian grounds. The American Zionist response to Nazism also shaped he political turmoil in the Middle East which followed Israel’s creation. Concerned primarily with providing a home for Jewish refugees and fearing British betrayal, Zionists could not understand Arab protests in defense of their own national interests. Instead they responded to the Arab revolt with armed force and sought to insure their own claim to Palestine, Zionists came to link he Arabs with the Nazi and British forces that were opposed to the establishment of a Jewish state. In the thinking of American Zionists, the Arabs were steadily transformed from a people with whom an accommodation would have to be made into a mortal enemy to be defeated. Aaron Berman does not apologize for American Jews, but rather tries to understand the constraints within which they operated and what opportunities-if any-they had to respond to Hitler. In surveying the latest scholarship and responding o charges against American Jewry, Berman’s arguments are reasoned and reasonable.
Author: Cam Patterson
Publisher: Springer Science & Business Media
ISBN: 1592598951
Category : Science
Languages : en
Pages : 376
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Book Description
A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.
Author: Shmuel Cabilly
Publisher: Springer Science & Business Media
ISBN: 1592595715
Category : Science
Languages : en
Pages : 320
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Book Description
During the course of evolution, an imbalance was created between the rate of vertebrate genetic adaptation and that of the lower forms of living organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutionary processes, not at the level of whole organism, but in specialized cells containing the genes that code for antibody molecules or for T-cell receptors. That is, when an immediate requirement for molecules capable of specific interactions arose, nature has preferred to speed up the mode of Darwinian evolution in pref- ence to any other approach (such as the use of X-ray diffraction studies and computergraphic analysis). Recently, Darwinian rules have been adapted for test tube research, and the concept of selecting molecules having particular characteristics from r- dom pools has been realized in the form of various chemical and biological combinatorial libraries. While working with these libraries, we noticed the interesting fact that when combinatorial libraries of oligopeptides were allowed to interact with different selector proteins, only the actual binding sites of these proteins showed binding properties, whereas the rest of the p- tein surface seemed "inert. " This seemingly common feature of protein- having no extra potential binding sites--was probably selected during evolution in order to minimize nonspecific interactions with the surrounding milieu.
Author: Nicholas Meyer
Publisher: Minotaur Books
ISBN: 1250237114
Category : Fiction
Languages : en
Pages : 256
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Book Description
With the international bestseller The Seven-Per-Cent Solution, Nicholas Meyer brought to light a previously unpublished case of Sherlock Holmes, as recorded by Dr. John H. Watson. Now Meyer returns with a shocking discovery—an unknown case drawn from a recently unearthed Watson journal. January 1905: Holmes and Watson are summoned by Holmes' brother Mycroft to undertake a clandestine investigation. An agent of the British Secret Service has been found floating in the Thames, carrying a manuscript smuggled into England at the cost of her life. The pages purport to be the minutes of a meeting of a secret group intent on nothing less than taking over the world. Based on real events, the adventure takes the famed duo—in the company of a bewitching woman—aboard the Orient Express from Paris into the heart of Tsarist Russia, where Holmes and Watson attempt to trace the origins of this explosive document. On their heels are desperate men of unknown allegiance, determined to prevent them from achieving their task. And what they uncover is a conspiracy so vast as to challenge Sherlock Holmes as never before.
Author: Marc De Ley
Publisher: Springer Science & Business Media
ISBN: 1592596673
Category : Medical
Languages : en
Pages : 248
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Book Description
A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Enjoy a quick and smooth introduction to the key methods used in cytokine research Use readily reproducible techniques that ensure successful experimental results Employ antisense-RNA, RT-PCR of small amounts of mRNA, and in situ hybridization.
Author: Aaron Berman
Publisher: Wayne State University Press
ISBN: 9780814322321
Category : Holocaust, Jewish (1939-1945)
Languages : en
Pages : 244
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Book Description
An investigation of the response of American Jews to Nazism and the extermination of European Jewry. The demand for Jewish statehood politicized the rescue issue and made it impossible to appeal for American aid on purely humanitarian grounds. Berman tries to understand the constraints within which American Jews operated. Annotation copyrighted by Book News, Inc., Portland, OR
