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Author: P.R. Stewart
Publisher: Springer Science & Business Media
ISBN: 3662004992
Category : Medical
Languages : en
Pages : 280
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Book Description
The central role of the ribonucleic acids (RNA) in mediating the expression of information encoded in DNA in living cells is now well established. Research in this area of biology continues at a remarkable rate, and new and significant information appears almost daily in a wide range of journals, published symposia and specialist reviews. The scattered nature of this information makes it difficult for the newcomer to the field of ribonucleic acid biochemistry to obtain a general oversight of current activity and new advances. Moreover, the reviews available for the most part are concerned with rather insular aspects of these ubiquitous molecules, or in the case of text-books, the subject is treated as part of a general out line of properties of nucleic acids and thus often tends to be superficial. With these considerations in mind, a postgraduate course was instituted in the university in Canberra to attempt to provide a comprehensive, though not excessively detailed, outline of the biological roles of RNA. The course was designed for students with a sound undergraduate training in biochemistry, but other wise with a wide variety of biological interests-plant physiology, virology, organelle biochemistry, genetics. The chapters in this book represent the matter of that course.
Author: P.R. Stewart
Publisher: Springer Science & Business Media
ISBN: 3662004992
Category : Medical
Languages : en
Pages : 280
Get Book Here
Book Description
The central role of the ribonucleic acids (RNA) in mediating the expression of information encoded in DNA in living cells is now well established. Research in this area of biology continues at a remarkable rate, and new and significant information appears almost daily in a wide range of journals, published symposia and specialist reviews. The scattered nature of this information makes it difficult for the newcomer to the field of ribonucleic acid biochemistry to obtain a general oversight of current activity and new advances. Moreover, the reviews available for the most part are concerned with rather insular aspects of these ubiquitous molecules, or in the case of text-books, the subject is treated as part of a general out line of properties of nucleic acids and thus often tends to be superficial. With these considerations in mind, a postgraduate course was instituted in the university in Canberra to attempt to provide a comprehensive, though not excessively detailed, outline of the biological roles of RNA. The course was designed for students with a sound undergraduate training in biochemistry, but other wise with a wide variety of biological interests-plant physiology, virology, organelle biochemistry, genetics. The chapters in this book represent the matter of that course.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Robert E. Farrell Jr.
Publisher: Elsevier
ISBN: 0080454763
Category : Science
Languages : en
Pages : 794
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Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author: Ralph Rapley
Publisher: Humana Press
ISBN: 9781489942500
Category : Science
Languages : en
Pages : 264
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Book Description
Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
Author: Jean Brachet
Publisher:
ISBN:
Category : Biosynthesis
Languages : en
Pages : 160
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Book Description
Author: Ioly Kotta-Loizou
Publisher: Springer Nature
ISBN: 3030765717
Category : Science
Languages : en
Pages : 180
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Book Description
Ribonucleic acid (RNA) is a macromolecule that plays a central role in cell physiology: RNA molecules act as intermediates between the deoxyribonucleic acid (DNA), where genetic information is stored, and proteins, which perform the necessary functions within the cell. Traditionally, the structural and functional properties of RNA are closely linked to gene expression. However, RNA-based enzymes, called ribozymes, are also involved in catalysis and small RNAs regulate key cellular processes, such as cell growth, division, differentiation, aging and death. RNA is a sensitive macromolecule that can be easily damaged by environmental conditions (ultraviolet radiation, oxidative stress) and biological factors (ribonucleases, ribotoxins, CRISPR-Cas systems). Therefore, cells have developed mechanisms to protect and/or repair RNA molecules. This book presents an overview of the biology of RNA damage, protection and repair in prokaryotes and eukaryotes. Individual chapters cover the expression regulation, enzymology and physiological role of such systems, and link them to important human diseases such as cancer and degenerative diseases.
Author: Roland K. Hartmann
Publisher: John Wiley & Sons
ISBN: 3527327762
Category : Science
Languages : en
Pages : 1370
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Book Description
The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.
Author: Thomas R. Cech
Publisher:
ISBN: 9781621822240
Category : Science
Languages : en
Pages : 580
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Book Description
"A Subject Collection from Cold Spring Harbor Perspectives in Biology."
Author: Nina T. Parker
Publisher:
ISBN: 9781947172791
Category : Microbiology
Languages : en
Pages : 0
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Book Description
Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs.
Author:
Publisher: Academic Press
ISBN: 0128171944
Category : Medical
Languages : en
Pages : 444
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Book Description
RNA-based Regulation in Human Health and Disease offers an in-depth exploration of RNA mediated genome regulation at different hierarchies. Beginning with multitude of canonical and non-canonical RNA populations, especially noncoding RNA in human physiology and evolution, further sections examine the various classes of RNAs (from small to large noncoding and extracellular RNAs), functional categories of RNA regulation (RNA-binding proteins, alternative splicing, RNA editing, antisense transcripts and RNA G-quadruplexes), dynamic aspects of RNA regulation modulating physiological homeostasis (aging), role of RNA beyond humans, tools and technologies for RNA research (wet lab and computational) and future prospects for RNA-based diagnostics and therapeutics. One of the core strengths of the book includes spectrum of disease-specific chapters from experts in the field highlighting RNA-based regulation in metabolic & neurodegenerative disorders, cancer, inflammatory disease, viral and bacterial infections. We hope the book helps researchers, students and clinicians appreciate the role of RNA-based regulation in genome regulation, aiding the development of useful biomarkers for prognosis, diagnosis, and novel RNA-based therapeutics. Comprehensive information of non-canonical RNA-based genome regulation modulating human health and disease Defines RNA classes with special emphasis on unexplored world of noncoding RNA at different hierarchies Disease specific role of RNA - causal, prognostic, diagnostic and therapeutic Features contributions from leading experts in the field
