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Author: Gregg G. Gundersen
Publisher: Humana
ISBN: 9781493993673
Category : Science
Languages : en
Pages : 338
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Book Description
This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Gregg G. Gundersen
Publisher:
ISBN: 9781493986910
Category : Cytoskeleton
Languages : en
Pages : 338
Get Book Here
Book Description
Author: Gregg G. Gundersen
Publisher: Humana
ISBN: 9781493993673
Category : Science
Languages : en
Pages : 338
Get Book Here
Book Description
This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Brian Alexander Sosa-Alvarado
Publisher:
ISBN:
Category :
Languages : en
Pages : 111
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Book Description
The nucleus is the hallmark of the eukaryotic cell. It contains most of the genetic material and it separates the processes of replication and transcription from that of translation. Communication between the nucleus and the cytoplasm occurs mostly through openings in the nuclear envelope composed of nuclear pore complexes. These massive assemblies allow for regulated transport of macromolecules across the barrier that is the nuclear envelope. However, another means of communication between the nucleus and the cytoskeleton has been characterized: linkers of the nucleoskeleton and cytoskeleton, or LINC, complexes mechanically connect the nucleus with its surroundings allowing for nuclear anchorage and nuclear movement during development and chromosome movement during meiosis. At the heart of LINC complexes are inner nuclear membrane resident SUN proteins and outer nuclear membrane resident KASH proteins. In this thesis we structurally characterize the human SUN2-KASH1 and KASH2 complexes and provide the molecular basis for their interaction. The solved structures suggest plausible models for high-order of LINC complex assembly as well as LINC complex mediated spacing between the inner and outer nuclear membrane. Questions about how these complexes are regulated also arise from the structure. TorsinA is an AAA+ ATPase suggested to play a role in LINC complex regulation. Analysis of Torsin's binding partners LAP1 and LULL1 show that they are catalytically inactive AAA+ ATPases. We characterize the complex and show by EM that they form ring akin to other AAA+ ATPases. With these studies we provide the first structural analysis of LINC complexes and Torsin ATPases and also provide biochemical tools for the study of LINC complex regulation.
Author: Xuanzong Guo
Publisher:
ISBN:
Category :
Languages : en
Pages : 38
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Book Description
LINC complexes span the nuclear envelope and connect the nucleoskeleton to the cytoskeleton. In 2012, our lab solved the first LINC complex structure, that of SUN domain of human SUN2 bound with KASH1 or KASH2 peptides. In this project testes-specific human SUN proteins (SUN3, SPAG4, and SUNS) were compared to ubiquitously-expressed SUN2. Secondly, fission and budding yeast LINC complexes differ from human ones and were analyzed as well. I was able to confirm SUN-KASH interaction in human and yeast. For structural analysis I explored various expression strategies. Fic-1 is a C. elegans Fic-domain protein with diverse cellular functions. As a subfamily III Fic enzyme, Fic-1 may reveal valuable insights into Fic enzyme mechanisms from its structure. After trying different knowledge-informed constructs and crystal optimization, small Fic-1 crystals were obtained, which diffracted X-rays to ~ 7 [angstroms]. With modest additional effort diffraction-quality crystals should be achievable.
Author: Davide Vigetti
Publisher: Humana Press
ISBN: 9781493991327
Category : Science
Languages : en
Pages : 278
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Book Description
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.
Author:
Publisher: Academic Press
ISBN: 0123973473
Category : Science
Languages : en
Pages : 1081
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Book Description
The genetic, molecular, and cellular mechanisms of neural development are essential for understanding evolution and disorders of neural systems. Recent advances in genetic, molecular, and cell biological methods have generated a massive increase in new information, but there is a paucity of comprehensive and up-to-date syntheses, references, and historical perspectives on this important subject. The Comprehensive Developmental Neuroscience series is designed to fill this gap, offering the most thorough coverage of this field on the market today and addressing all aspects of how the nervous system and its components develop. Particular attention is paid to the effects of abnormal development and on new psychiatric/neurological treatments being developed based on our increased understanding of developmental mechanisms. Each volume in the series consists of review style articles that average 15-20pp and feature numerous illustrations and full references. Volume 2 offers 56 high level articles devoted mainly to Formation of Axons and Dendrites, Migration, Synaptogenesis, Developmental Sequences in the Maturation of Intrinsic and Synapse Driven Patterns. Series offers 144 articles for 2904 full color pages addressing ways in which the nervous system and its components develop Features leading experts in various subfields as Section Editors and article Authors All articles peer reviewed by Section Editors to ensure accuracy, thoroughness, and scholarship Volume 2 sections include coverage of mechanisms which regulate: the formation of axons and dendrites, cell migration, synapse formation and maintenance during development, and neural activity, from cell-intrinsic maturation to early correlated patterns of activity
Author: Loic Gazquez
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Peter Fantes
Publisher:
ISBN: 9781383049756
Category : Cell nuclei
Languages : en
Pages : 0
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Book Description
Author:
Publisher: Academic Press
ISBN: 9780128034705
Category : Science
Languages : en
Pages : 0
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Book Description
Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.
Author: Diller Scofidio + Renfro
Publisher: Damiani Limited
ISBN: 9788862082440
Category : Architecture
Languages : en
Pages : 0
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Book Description
The redesign of Lincoln Center is one of the most challenging and innovative civic projects in recent urban history. Over the past eight years Diller Scofi dio + Renfro, in close collaboration with Lincoln Center's leadership, has transformed the fi fty year old Modernist citadel into a porous and democratic campus. This visually rich document is the first comprehensive book to feature the extensive redevelopment in its entirety. Through a combination of photographs, drawings, renderings, archival records and texts, the book describes the innovative strategies that have dissolved the public/private divide and effectively turned the campus inside-out, extending the spectacle of the performance halls into the Center's mute public spaces and surrounding streets. Conceived as a cross between an art book, a scholarly record, and an architectural diary this publication demonstrates how the recent redesign both respects and challenges preconceived notions about Lincoln Center and its ongoing role as a cultural hub in an ever-changing city. This unorthodox publication is comprised entirely of gatefolds; a series of inside-out centerfolds where the exterior pages of each spread feature glossy, large-format, full-bleed photographs highlighting different parts of the campus. Inside the gatefolds, tucked behind these lush photos, is a series of "back stories" that reveal the surprising evolution and unexpected afterlife of the same spaces.
