Author: Elizabeth Chen
Publisher:
ISBN:
Category :
Languages : en
Pages : 574
Book Description
The Identification and Characterization of a Gene Encoding a Novel Actin Associated Protein that is Required for Microfilament Distribution in Saccharomyces Cerevisiae
Author: Elizabeth Chen
Publisher:
ISBN:
Category :
Languages : en
Pages : 574
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 574
Book Description
Identification and Characterization of Proteins Important for Actin Cytoskeletal Function in the Yeast Saccharomyces Cerevisiae
Author: Matthew Dunbar Welch
Publisher:
ISBN:
Category :
Languages : en
Pages : 308
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 308
Book Description
Characterization of Actin-related Proteins in Vertebrates and the Yeast Saccharomyces Cerevisiae
Author: Sean William Clark
Publisher:
ISBN:
Category :
Languages : en
Pages : 300
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 300
Book Description
Identification and characterization of profilin-associated proteins in Saccharomyces cerevisiae
Author: Stephen Joseph Palmieri
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 360
Book Description
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 360
Book Description
The Identification and Characterization of New Genetic Requirements for Proper Cell Type Identity in Saccharomyces Cerevisiae
Author: Sonia Santa Anna-A.
Publisher:
ISBN:
Category :
Languages : en
Pages : 368
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 368
Book Description
Identification and Characterization of a Novel Gene Affecting the Transcript Level of an ATP-binding Cassette Transporter Gene, PDR5, in Saccharomyces Cerevisiae
Author: Dwayne William Dexter
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 186
Book Description
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 186
Book Description
Cloning and Characterization of the Genes Encoding P33 and P135 Subunits of Translation Initiation Factor 3 in Saccharomyces Cerevisiae
Author: Parisa Hanachi
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
Book Description
Characterization of Proteins Required for Spindle Formation and Orientation in Saccharomyces Cerevisiae
Author: Hongwei Yin
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
Identification and Characterization of Novel Proteins and Pathways for MRNA Degradation and Quality Control in Saccharomyces Cerevisiae
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
Book Description
In eukaryotes, mRNA decay pathways are important for cellular response to various physiological conditions and also function in co-translational quality control systems that target translationally aberrant mRNAs for degradation. My work on identification and characterization of novel components and pathways of mRNA degradation and quality control in Saccharomyces cerevisiae is summarized below. I have identified Edc3p as a novel protein important for mRNA decay. Deletion of Edc3p leads to a defect in mRNA decay in strains deficient in decapping enzymes and, in combination with a block to the 3' to 5' decay pathway, causes exaggerated growth defects and synthetic lethality. An Edc3p-GFP fusion protein localizes in processing bodies, which are specialized cytoplasmic foci containing decapping proteins. Together, these observations indicate that Edc3p directly interacts with the decapping complex to stimulate the mRNA decapping rate. Quality control during mRNA translation is critical for regulation of gene expression. My work shows that yeast mRNAs with defects in translation elongation, due to strong translational pauses, are recognized and targeted for degradation via an endonucleolytic cleavage in a novel process referred to as No-Go Decay (NGD). The cellular mRNA decay machinery degrades the 5' and 3' cleavage products produced by NGD. NGD is translation-dependent, occurs in a range of mRNAs and can be induced by a variety of elongation pauses. These results indicate NGD may occur at some rate inresponse to any stalled ribosome. I also show that two highly conserved proteins, Dom34p and Hbs1p, homologous to the eukaryotic release factors eRF1 and eRF3 respectively, are required for NGD. Further characterization of the No-Go decay pathway indicates that Dom34p function during NGD is conserved across species. Identification of RPS30, a small ribosomal protein as a trans-acting factor during NGD suggests that the ribosome may have a novel role during NGD. Other experiments indicate that the No-Go decay pathway may cross talk with the unfolded protein response pathway. The identification of No-Go decay as a novel quality control pathway during translation elongation supports the existence of a global cellular mechanism for maintenance of translational quality control.
Publisher:
ISBN:
Category :
Languages : en
Pages : 270
Book Description
In eukaryotes, mRNA decay pathways are important for cellular response to various physiological conditions and also function in co-translational quality control systems that target translationally aberrant mRNAs for degradation. My work on identification and characterization of novel components and pathways of mRNA degradation and quality control in Saccharomyces cerevisiae is summarized below. I have identified Edc3p as a novel protein important for mRNA decay. Deletion of Edc3p leads to a defect in mRNA decay in strains deficient in decapping enzymes and, in combination with a block to the 3' to 5' decay pathway, causes exaggerated growth defects and synthetic lethality. An Edc3p-GFP fusion protein localizes in processing bodies, which are specialized cytoplasmic foci containing decapping proteins. Together, these observations indicate that Edc3p directly interacts with the decapping complex to stimulate the mRNA decapping rate. Quality control during mRNA translation is critical for regulation of gene expression. My work shows that yeast mRNAs with defects in translation elongation, due to strong translational pauses, are recognized and targeted for degradation via an endonucleolytic cleavage in a novel process referred to as No-Go Decay (NGD). The cellular mRNA decay machinery degrades the 5' and 3' cleavage products produced by NGD. NGD is translation-dependent, occurs in a range of mRNAs and can be induced by a variety of elongation pauses. These results indicate NGD may occur at some rate inresponse to any stalled ribosome. I also show that two highly conserved proteins, Dom34p and Hbs1p, homologous to the eukaryotic release factors eRF1 and eRF3 respectively, are required for NGD. Further characterization of the No-Go decay pathway indicates that Dom34p function during NGD is conserved across species. Identification of RPS30, a small ribosomal protein as a trans-acting factor during NGD suggests that the ribosome may have a novel role during NGD. Other experiments indicate that the No-Go decay pathway may cross talk with the unfolded protein response pathway. The identification of No-Go decay as a novel quality control pathway during translation elongation supports the existence of a global cellular mechanism for maintenance of translational quality control.
Identification and Characterization of Genes Required for Early Meiotic Gene Expression in the Yeast Saccharomyces Cerevisiae
Author: Sophia Seh-Yi Su
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
Book Description