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Author: John R. Crowther
Publisher: Humana
ISBN: 9781617378843
Category : Medical
Languages : en
Pages : 0
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Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: John R. Crowther
Publisher: Humana
ISBN: 9781617378843
Category : Medical
Languages : en
Pages : 0
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Book Description
There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Author: Samira Hosseini
Publisher: Springer
ISBN: 981106766X
Category : Technology & Engineering
Languages : en
Pages : 124
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Book Description
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Author: Robert Hnasko
Publisher: Humana
ISBN: 9781493927418
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Author: David Wild
Publisher: Newnes
ISBN: 0080970389
Category : Science
Languages : en
Pages : 1037
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Book Description
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Author: John R. Crowther
Publisher: Springer Science & Business Media
ISBN: 1592595294
Category : Medical
Languages : en
Pages : 231
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Book Description
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Author: John R. Crowther
Publisher: Springer Science & Business Media
ISBN: 1592590497
Category : Science
Languages : en
Pages : 426
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Book Description
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Author: Elisa Donovan
Publisher: Llewellyn Worldwide
ISBN: 0738768383
Category : Body, Mind & Spirit
Languages : en
Pages : 156
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Book Description
Love, Grief, and Healing in Hollywood and Beyond After prominent roles in Clueless; Sabrina, the Teenage Witch; and Beverly Hills 90210; Elisa's career was on the fast track. Until her show is unexpectedly cancelled, her relationship ends, and her father is diagnosed with terminal cancer. This book chronicles Elisa's journey out of despair and heartbreak, with awe-inspiring visitations, dreams, and inexplicable synchronicities that could only be her father letting her know that he's watching over her from the afterlife. Sometimes the universe sends us on a journey that we didn't know we needed. By sharing the lessons and challenges that the universe sent to her, Elisa inspires those who are learning to let go after a loss so they can live again with authenticity, humor, and hope.
Author: Elisa Albert
Publisher: Houghton Mifflin Harcourt
ISBN: 0544273737
Category : Fiction
Languages : en
Pages : 211
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Book Description
A widely acclaimed young writer's fierce new novel, in which childbirth and new motherhood are as high-stakes a crucible as any combat zone.
Author: Dr. Elisa Port
Publisher: Ballantine Books
ISBN: 1101883146
Category : Health & Fitness
Languages : en
Pages : 322
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Book Description
From an expert in the field comes the definitive guide to managing breast cancer in the information age—a comprehensive resource for diagnosis, treatment, and peace of mind. The breast cancer cure rate is at an all-time high, and so is the information, to say nothing of the misinformation, available to patients and their families. Online searches can lead to unreliable sources, leaving even the most resilient patient feeling uneasy and uncertain about her diagnosis, treatment options, doctors, side effects, and recovery. Adding to a patient’s anxiety is input from well-meaning friends and family, with stories, worries, and opinions to share, sometimes without knowing the details of her particular case, when in reality breast cancer treatment has gone well beyond a “one size fits all” approach. Elisa Port, MD, FACS, chief of breast surgery at The Mount Sinai Hospital and co-director of the Dubin Breast Center in Manhattan, offers an optimistic antidote to the ocean of Web data on screening, diagnosis, prognosis, and treatment. Inside you’ll discover • the various scenarios when mammograms indicate the need for a biopsy • the questions to ask about surgery, chemotherapy, radiation, and breast reconstruction • the important things to look for when deciding where to get care • the key to deciphering complicated pathology reports and avoiding confusion • the facts on genetic testing and the breast cancer genes: BRCA-1 and BRCA-2 • the best resources and advice for those supporting someone with breast cancer From innovations in breast cancer screening and evaluating results to post-treatment medications and living as a breast cancer survivor, Dr. Elisa Port describes every possible test and every type of doctor visit, providing a comprehensive, empathetic guide that every newly diagnosed woman (and her family) will want to have at her side. Praise for The New Generation Breast Cancer Book “One book you need . . . If you’re considering your options for treatment or know someone who is, this step-by-step guide, The New Generation Breast Cancer Book, is essential reading.”—InStyle “Elisa Port, M.D., is the doctor every patient deserves: brilliant and compassionate. Her book will be a sanity saver and, quite possibly, a life saver.”—Geralyn Lucas, author of Why I Wore Lipstick to My Mastectomy “As up-to-date as one can get, with lots to offer people facing a cancer diagnosis or hoping to support someone with the disease.”—Library Journal (starred review) “The New Generation Breast Cancer Book helps you sort through all the information you’ve gathered, clarify the terminology, consider the options, and make the right decisions for your unique case.”—Edie Falco “A lifeline for many women in need of today’s most up-to-date choices for treatment . . . Everyone should read this book for themselves, their mothers, grandmothers, daughters, and friends.”—Kara DioGuardi, Grammy-nominated songwriter, music executive, and Arthouse Entertainment co-founder “The book is teeming with easy-to-understand medical explanations, tips, takeaways, and pro-and-con discussions of various courses of action. Port also includes two extremely useful appendices that respectively take on common myths and answer questions frequently asked by friends and family. This is a vital read that will empower men and women alike.”—Publishers Weekly
Author: D. M. Kemeny
Publisher: John Wiley & Sons
ISBN: 9780471909828
Category : Science
Languages : en
Pages : 384
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Book Description
This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
