In Vitro Cultivation of Parasitic Helminths (1990) PDF Download
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Author: James D. Smyth
Publisher: CRC Press
ISBN: 135136149X
Category : Science
Languages : en
Pages : 426
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Book Description
A critical account of the available techniques for the in vitro cultivation of parasitic helminths (Trematoda, Cestoda, Nematoda, and Acanthocephala), concentrating on those which have been reasonably successful and can be used for teaching or research purposes. In addition to describing basic techn
Author: James D. Smyth
Publisher: CRC Press
ISBN: 135136149X
Category : Science
Languages : en
Pages : 426
Get Book Here
Book Description
A critical account of the available techniques for the in vitro cultivation of parasitic helminths (Trematoda, Cestoda, Nematoda, and Acanthocephala), concentrating on those which have been reasonably successful and can be used for teaching or research purposes. In addition to describing basic techn
Author: Patsy Jenson
Publisher: CRC Press
ISBN: 1351090356
Category : Science
Languages : en
Pages : 525
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Book Description
It is the purpose of this book to make available to parasitologists and workers in many other disciplines a review of the developments leading up to the successful cultivation of the more important protozoan parasites of man and domestic animals. Included is a detailed description of the current state of the art protozoan parasite cultivation, and a limited discussion of the major achievements to our understanding of parasite biology derived through experimentation using cultured parasites.
Author: Angela E. R. Taylor
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 516
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Book Description
Author: Angela Taylor
Publisher:
ISBN: 9780397601332
Category :
Languages : en
Pages : 392
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Book Description
Author: James B. Jensen
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 320
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 377
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Book Description
Author: Angela E. R. Taylor
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 344
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Book Description
Techniques and media commonly used for In vitro culture; Entamoeba, giardia and trichomonas; Rumen entodiniomorphid protozoa; Kinetoplastida; Plasmodiidae; Cell and tissue culture; Chicken embryos; Trematoda; Cestóda; In vitro cultivation of nematodes parasitic in animals and plants; Acanthocephala.
Author: Angela Taylor
Publisher:
ISBN:
Category :
Languages : fr
Pages : 465
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Book Description
Author: Denise L. Doolan
Publisher: Springer Science & Business Media
ISBN: 1592592716
Category : Medical
Languages : en
Pages : 606
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Book Description
The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.
Author: Jenna West
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 54
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Book Description
Author's abstract: It is important to study the cultivation of parasites in vitro for many reasons, such as to aid in developing antihelminthic drugs and vaccines, to eliminate the need for vertebrate hosts in parasite culture, and to more easily study the genetics and the biology of parasites. Trematodes have complex life cycles with multiple hosts which makes them difficult to grow in vitro. However, microphallid trematodes are excellent candidates for parasite in vitro cultivation because they are short lived and progenetic. The goal of my study was to optimize in vitro culture conditions for the microphallid trematode, Gynaecotyla adunca. I determined the optimal concentration of trypsin to be 0.5% in order to excyst the most metacercariae of G. adunca that were obtained from the green glands of the second intermediate host, the fiddler crab Uca pugnax. Hunter (1952) reported that G. adunca only self-fertilize, however, offered no evidence to support this claim. I decided to test G. adunca adult worms to either confirm or refute whether this is true or not. I also tested different culture conditions on adult G. adunca worms. I chose 3 parameters to evaluate in vitro cultivation experiments. To determine these parameters which included worm longevity, number of worms that produced eggs in utero, and the number of eggs deposited, the media DMEM and RPMI-1640 were compared to Hank's Balanced Salt Solution. Different sera were also tested including horse, new-born calf, and chicken, the best of which was tested at different concentrations. To test the viability of the eggs deposited by worms in culture, they were fed to the marsh snail Ilyanassa obsoleta. I also compared HBSS and DMEM plus 5% horse serum as the initial incubation conditions of the freshly excysted worms then observed them 24 hr later before adding them to culture to see if this affected egg production. G. adunca worms do self-fertilize. When worms were incubated alone, they showed signs of being fertilized. The percentage of worms with eggs in utero was greatest when worms were grown in DMEM. Worms lived longer and deposited more eggs when cultured in DMEM supplemented with 5% horse serum. Snails fed eggs from culture were not successfully infected. There was no significant difference on egg production between initially incubating the worms in HBSS and DMEM plus 5% horse serum within the 24 hr period between excystment and adding them to culture. Future studies will further refine in vitroculture conditions for G. adunca and investigate the best approach for snail infection.
