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Author: Domenico Ribatti
Publisher: Springer
ISBN: 9789400791572
Category : Medical
Languages : en
Pages : 0
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Book Description
1. Introduction 2. Chorioallantoic membrane and its embryological origin 2.1. Morphology of chorioallantoic membrane blood and lymphatic vessels 2.2. A single blood sinus or a capillary plexus beneath the chorionic epithelium? 2.3. The chorioallantoic membrane vascular growth 3. Use of chorioallantoic membrane in the study of angiogenic molecules 3.1. Role of FGF-2 in chorioallantoic membrane vascularization 4. Use of chorioallantoic membrane in the study of antiangiogenic molecules 5. Use of chorioallantoic membranes in the study of tumor angiogenesis 5.1. Angiogenesis and antiangiogenesis in multiple myeloma 5.2. Angiogenesis and antiangiogenesis in human neuroblastoma 6. Use of chorioallantoic membrane in the study of tumor metastasis 6.1. Spontaneous metastasis models. 6.2. Experimental metastasis studies. 7. Other applications 7.1. In ovo and ex ovo methods 7.2. Use of chorioallantoic membrane in the study of tumor lymphangiogenesis 8. Different morphological techniques that can be used to study vascularization of the CAM and the genes involved. 9. Methods of quantifying the angiogenic response 10. Limitations of the chorioallantoic membrane assay 11. Other classical in vivo assays in comparison (advantages, disadvantages and limitations) with the chorioallantoic membrane 12. Concluding remarks Acknowledgments References
Author: Domenico Ribatti
Publisher: Springer
ISBN: 9789400791572
Category : Medical
Languages : en
Pages : 0
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Book Description
1. Introduction 2. Chorioallantoic membrane and its embryological origin 2.1. Morphology of chorioallantoic membrane blood and lymphatic vessels 2.2. A single blood sinus or a capillary plexus beneath the chorionic epithelium? 2.3. The chorioallantoic membrane vascular growth 3. Use of chorioallantoic membrane in the study of angiogenic molecules 3.1. Role of FGF-2 in chorioallantoic membrane vascularization 4. Use of chorioallantoic membrane in the study of antiangiogenic molecules 5. Use of chorioallantoic membranes in the study of tumor angiogenesis 5.1. Angiogenesis and antiangiogenesis in multiple myeloma 5.2. Angiogenesis and antiangiogenesis in human neuroblastoma 6. Use of chorioallantoic membrane in the study of tumor metastasis 6.1. Spontaneous metastasis models. 6.2. Experimental metastasis studies. 7. Other applications 7.1. In ovo and ex ovo methods 7.2. Use of chorioallantoic membrane in the study of tumor lymphangiogenesis 8. Different morphological techniques that can be used to study vascularization of the CAM and the genes involved. 9. Methods of quantifying the angiogenic response 10. Limitations of the chorioallantoic membrane assay 11. Other classical in vivo assays in comparison (advantages, disadvantages and limitations) with the chorioallantoic membrane 12. Concluding remarks Acknowledgments References
Author: Domenico Ribatti
Publisher: Academic Press
ISBN: 0128140216
Category : Science
Languages : en
Pages : 130
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Book Description
In Vivo Models to Study Angiogenesis provides the latest information and an overview of the most common assays for studying angiogenesis in vivo. Under physiological conditions, angiogenesis is tightly controlled, whereas increased production of angiogenic stimuli and/or reduced production of angiogenic inhibitors leads to abnormal neovascularization, such as occurs in cancer, chronic inflammatory disease, diabetic retinopathy, macular degeneration and cardiovascular disorders. Several genetic and epigenetic mechanisms are involved in the early development of the vascular system. This book presents the latest information from the extensive literature and research available. Evidence is now emerging that blood vessels themselves have the ability to provide instructive regulatory signals to surrounding non-vascular target cells during organ development. Thus, endothelial cell signaling is currently believed to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation and postnatal tissue remodeling. - Provides information on the most common assays to study angiogenesis in vivo - Presents an ideal reference for those interested in angiogenesis as a normal and vital process in growth and development - Covers wound healing, the formation of granulation tissue, and the transition of tumors from benign to malignant
Author: Carolyn A. Staton
Publisher: John Wiley & Sons
ISBN: 047002934X
Category : Medical
Languages : en
Pages : 410
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Book Description
Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Author: Domenico Ribatti
Publisher: Academic Press
ISBN: 0128194944
Category : Science
Languages : en
Pages : 198
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Book Description
Tumor Vascularization discusses the different types of growth of tumor blood vessels and their implications on research and healthcare. The book is divided into three parts: the first one, General Mechanisms, discusses different vessel growth mechanisms, such as sprouting angiogenesis, non-angiogenesis dependent growth, intussusceptive microvascular growth, vascular co-option and vasculogenic mimicry. The second and third parts, entitled Clinical Implications and Therapeutic Implications are dedicated to translating recent findings in this field to patient treatment and healthcare. This book is a valuable source for cancer researchers, oncologists, graduate students and members of the biomedical field who are interested in tumor progression and blood vessels.
Author: Mark Slevin
Publisher: Springer
ISBN: 9401797161
Category : Medical
Languages : en
Pages : 466
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Book Description
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here. Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work. Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.
Author: Susan A. Brooks
Publisher: Springer Science & Business Media
ISBN: 159259137X
Category : Medical
Languages : en
Pages : 310
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Book Description
In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.
Author: Domenico Ribatti
Publisher: Springer Science & Business Media
ISBN: 9048138450
Category : Medical
Languages : en
Pages : 130
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Book Description
The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morpho-functional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of pro-angiogenic and anti-angiogenic natural and synthetic molecules. The CAM is a suitable site for transplanting tissues, which can survive and develop in the CAM by peripheral anastomoses between graft and original CAM vasculature or by new angiogenic vessels grown from the CAM that invade the graft. While the formation of peripheral anastomoses between host and pre-existing donor vessels is the main, and the most common, mechanism involved in the revascularization of embryonic grafts, the growth of CAM-derived vessels into the graft is only stimulated in tumor grafts. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. Tumors remain avascular for 72 h, after which they are penetrated by new blood vessels and begin a phase of rapid growth. Also, delivery of tumor cells onto the CAM allows the fine study of the effects of tumor derived angiogenic growth factors on blood vessel structure and functionality. The CAM may also used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an anti-angiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.
Author: Jian Cao
Publisher: Humana
ISBN: 9781493934423
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.
Author: Shahid Ul Islam
Publisher: John Wiley & Sons
ISBN: 1119640423
Category : Science
Languages : en
Pages : 432
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Book Description
This book is organized into 12 important chapters that focus on the progress made by metal-based drugs as anticancer, antibacterial, antiviral, anti-inflammatory, and anti-neurodegenerative agents, as well as highlights the application areas of newly discovered metallodrugs. It can prove beneficial for researchers, investigators and scientists whose work involves inorganic and coordination chemistry, medical science, pharmacy, biotechnology and biomedical engineering.
Author: Maria del Mar Vivanco
Publisher: Humana
ISBN: 9781493925186
Category : Science
Languages : en
Pages : 0
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Book Description
The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
